Novel mechanisms of adaptive and innate bacteriophage immunity

Información del proyecto

DEFEND

Identificador del acuerdo de subvención: 101003229

DOI

10.3030/101003229

Fecha de la firma de la CE 4 Febrero 2021

Fecha de inicio 1 Junio 2021

Fecha de finalización 31 Mayo 2026

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 2 000 000,00

Aportación de la UE

€ 2 000 000,00

Coordinado por

TECHNISCHE UNIVERSITEIT DELFT
Netherlands

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Publicaciones

Peer reviewed articles (24)

KlebPhaCol: a community-driven resource for <i>Klebsiella</i> research identified a novel phage family

Autores: Daniela Rothschild-Rodriguez, Kai S Lambon, Simran Krishnakant Kushwaha, Sofya K Garushyants, Moritz Ertelt, Agnieszka Latka, Ana Rita Costa, Anna Mantzouratou, Claire King, Dimitri Boeckaerts, Elizab
Publicado en: Nucleic Acids Research, Edición 53, 2025, ISSN 0305-1048
Editor: Oxford University Press
DOI: 10.1093/nar/gkaf1122

Modulating CRISPR-Cas genome editing using guide-complementary DNA oligonucleotides

Autores: Thomas Swartjes; Peng Shang; Dennis van den Berg; Tim A. Künne; Niels Geijsen; Stan J.J. Brouns; John van der Oost; Raymond H.J. Staals; Richard A. Notebaart
Publicado en: The CRISPR Journal, 2022, ISSN 2573-1599
Editor: Mary Ann Liebert
DOI: 10.1101/2022.01.15.475214

Structural basis for broad anti-phage immunity by DISARM

Autores: Jack P. K. Bravo; Cristian Aparicio-Maldonado; Franklin L. Nobrega; Stan J. J. Brouns; David W. Taylor
Publicado en: Nature Communications, 2022, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1101/2021.12.26.474123

Phage tRNAs evade tRNA-targeting host defenses through anticodon loop mutations

Autores: Daan F. van den Berg; Baltus A. van der Steen; Ana Rita Costa; Stan J. J. Brouns
Publicado en: eLife, 2023, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.21203/rs.3.rs-2166710/v1

Bacteriophage genomes encode both broad and specific counter-defense repertoires to overcome bacterial defense systems

Autores: Ana Rita Costa, Daan F. van den Berg, Jelger Q. Esser, Halewijn van den Bossche, Nadiia Pozhydaieva, Konstantinos Kalogeropoulos, Stan J.J. Brouns
Publicado en: Cell Host &amp; Microbe, Edición 33, 2025, Página(s) 1161-1172.e5, ISSN 1931-3128
Editor: Cell Press
DOI: 10.1016/j.chom.2025.06.010

Craspase is a CRISPR RNA-guided, RNA-activated protease

Autores: Chunyi Hu; Sam P. B. van Beljouw; Ki Hyun Nam; Gabriel Schuler; Fran Ding; Yanru Cui; Alicia Rodríguez-Molina; Anna C. Haagsma; Menno Valk; Martin Pabst; Stan J. J. Brouns; Ailong Ke
Publicado en: Science, 2022, ISSN 0036-8075
Editor: American Association for the Advancement of Science
DOI: 10.1126/science.add5064

Single cell variability of CRISPR‐Cas interference and adaptation

Autores: Rebecca E McKenzie, Emma M Keizer, Jochem N A Vink, Jasper van Lopik, Ferhat Büke, Vera Kalkman, Christian Fleck, Sander J Tans, Stan J J Brouns
Publicado en: Molecular Systems Biology, 2022, ISSN 1744-4292
Editor: Nature Publishing Group
DOI: 10.15252/msb.202110680

An OmpW-dependent T4-like phage infects Serratia sp. ATCC 39006

Autores: Marina Mahler; Lucia M. Malone; Daan F. van den Berg; Leah M. Smith; Stan J. J. Brouns; Peter C. Fineran
Publicado en: Microbial Genomics, 2023, ISSN 2057-5858
Editor: London Society for General Microbiology
DOI: 10.1099/mgen.0.000968

Short prokaryotic Argonaute systems trigger cell death upon detection of invading DNA

Autores: Balwina Koopal; Ana Potocnik; Sumanth K. Mutte; Cristian Aparicio-Maldonado; Simon Lindhoud; Jacques J.M. Vervoort; Stan J.J. Brouns; Daan C. Swarts
Publicado en: Cell, 2022, ISSN 1097-4172
Editor: Cell press
DOI: 10.1016/j.cell.2022.03.012

Bacterial homologs of innate eukaryotic antiviral defenses with anti-phage activity highlight shared evolutionary roots of viral defenses

Autores: Daan F. van den Berg, Ana Rita Costa, Jelger Q. Esser, Ilinka Stanciu, Jasper Q. Geissler, Adja Damba Zoumaro-Djayoon, Pieter-Jan Haas, Stan J.J. Brouns
Publicado en: Cell Host &amp; Microbe, Edición 32, 2025, Página(s) 1427-1443.e8, ISSN 1931-3128
Editor: Cell Press
DOI: 10.1016/j.chom.2024.07.007

Mechanisms and clinical importance of bacteriophage resistance.

Autores: Julia E Egido; Ana Rita Costa; Ana Rita Costa; Cristian Aparicio-Maldonado; Cristian Aparicio-Maldonado; Pieter-Jan A. Haas; Stan J. J. Brouns; Stan J. J. Brouns
Publicado en: FEMS Microbiology Reviews, 2022, ISSN 1574-6976
Editor: Elsevier
DOI: 10.1093/femsre/fuab048

Phage arabinosyl-hydroxy-cytosine DNA modifications result in distinct evasion and sensitivity responses to phage defense systems

Autores: Marina Mahler, Liang Cui, Leah M. Smith, Katharina G. Wandera, Oliver Dietrich, David Mayo-Muñoz, Seetharamsing Balamkundu, Megan En Lee, Hong Ye, Chuan-Fa Liu, Junzhou Wu, Juby Mathew, Jeremy Dubrul
Publicado en: Cell Host &amp; Microbe, Edición 33, 2025, Página(s) 1173-1190.e9, ISSN 1931-3128
Editor: Cell Press
DOI: 10.1016/j.chom.2025.06.005

Reduced prevalence of phage defense systems in <i>Pseudomonas aeruginosa</i> strains from cystic fibrosis patients

Autores: Daan F. van den Berg, Stan J. J. Brouns
Publicado en: mBio, Edición 16, 2025, ISSN 2150-7511
Editor: American Society for Microbiology
DOI: 10.1128/mbio.03548-24

CasPEDIA Database: a functional classification system for class 2 CRISPR-Cas enzymes

Autores: Benjamin A Adler; Marena I Trinidad; Daniel Bellieny-Rabelo; Elaine Zhang; Hannah M Karp; Petr Skopintsev; Brittney W Thornton; Rachel F Weissman; Peter H Yoon; LinXing Chen; Tomas Hessler; Amy R Eggers; David Colognori; Ron Boger; Erin E Doherty; Connor A Tsuchida; Ryan V Tran; Laura Hofman; Honglue Shi; Kevin M Wasko; Zehan Zhou; Chenglong Xia; Muntathar J Al-Shimary; Jaymin R Patel; Vienna C J
Publicado en: Nucleic Acids Research, 2023, ISSN 1362-4962
Editor: Oxford University Press
DOI: 10.1093/nar/gkad890

Proteases in bacteriophage defense systems and their potential in bioengineering

Autores: Konstantinos Kalogeropoulos, Sam P.B. van Beljouw, Dani Feldmann, Daan F. van den Berg, Stan J.J. Brouns
Publicado en: Trends in Biochemical Sciences, Edición 51, 2026, Página(s) 64-79, ISSN 0968-0004
Editor: Elsevier BV
DOI: 10.1016/j.tibs.2025.10.006

Craspase Orthologs Cleave a Nonconserved Site in Target Protein Csx30

Autores: Sam P. B. van Beljouw, Anna C. Haagsma, Konstantinos Kalogeropoulos, Martin Pabst, Stan J. J. Brouns
Publicado en: ACS Chemical Biology, Edición 19, 2024, Página(s) 1051-1055, ISSN 1554-8929
Editor: American Chemical Society
DOI: 10.1021/acschembio.3c00788

The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with a caspase-like peptidase.

Autores: Sam P. B. van Beljouw; Sam P. B. van Beljouw; Anna C. Haagsma; Anna C. Haagsma; Alicia Rodríguez-Molina; Alicia Rodríguez-Molina; Daan F. van den Berg; Daan F. van den Berg; Jochem N.A. Vink; Jochem N.A. Vink; Stan J. J. Brouns; Stan J. J. Brouns
Publicado en: Science, 2021, ISSN 0036-8075
Editor: American Association for the Advancement of Science
DOI: 10.1126/science.abk2718

Adaptation by Type V-A and V-B CRISPR-Cas Systems Demonstrates Conserved Protospacer Selection Mechanisms Between Diverse CRISPR-Cas Types

Autores: Wen Y. Wu; Simon A. Jackson; Cristóbal Almendros; Anna C. Haagsma; Suzan Yilmaz; Gerrit Gort; John van der Oost; Stan J.J. Brouns; Raymond H.J. Staals
Publicado en: The CRISPR Journal, 2022, ISSN 2573-1602
Editor: Mary Ann Liebert
DOI: 10.1089/crispr.2021.0150

PAM-repeat associations and spacer selection preferences in single and co-occurring CRISPR-Cas systems.

Autores: Jochem N.A. Vink; Jochem N.A. Vink; Jan H. L. Baijens; Jan H. L. Baijens; Stan J. J. Brouns; Stan J. J. Brouns
Publicado en: Genome Biology, 2021, ISSN 1474-760X
Editor: London BioMed Central
DOI: 10.1186/s13059-021-02495-9

RNA-targeting CRISPR–Cas systems

Autores: Sam P. B. van Beljouw, Jasper Sanders, Alicia Rodríguez-Molina, Stan J. J. Brouns
Publicado en: Nature Reviews Microbiology, Edición 21, 2022, Página(s) 21-34, ISSN 1740-1526
Editor: Nature Publishing Group
DOI: 10.1038/s41579-022-00793-y

Monitoring phage-induced lysis of Gram-negatives in real time using a fluorescent DNA dye

Autores: Julia E. Egido; Catherine Toner-Bartelds; Ana Rita Costa; Stan J. J. Brouns; Suzan H. M. Rooijakkers; Bart W. Bardoel; Pieter-Jan Haas
Publicado en: Scientific Reports, 2023, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-023-27734-w

Approaches for bacteriophage genome engineering

Autores: Marina Mahler, Ana Rita Costa, Sam P.B. van Beljouw, Peter C. Fineran, Stan J.J. Brouns
Publicado en: Trends in Biotechnology, Edición 41, 2025, Página(s) 669-685, ISSN 0167-7799
Editor: Elsevier BV
DOI: 10.1016/j.tibtech.2022.08.008

High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers

Autores: Ruochen Ouyang; Ana Rita Costa; C. Keith Cassidy; Aleksandra Otwinowska; Vera C. J. Williams; Agnieszka Latka; Phill J. Stansfeld; Zuzanna Drulis-Kawa; Yves Briers; Daniël M. Pelt; Stan J. J. Brouns; Ariane Briegel
Publicado en: Nature Communications, 2022, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-022-34972-5

Accumulation of defense systems in phage-resistant strains of Pseudomonas aeruginosa

Autores: Costa AR, van den Berg DF, Esser JQ, Muralidharan A, van den Bossche H, Bonilla BE, van der Steen BA, Haagsma AC, Fluit AC, Nobrega FL, Haas PJ, Brouns SJJ
Publicado en: Science Advances, 2024, ISSN 2375-2548
Editor: Washington, DC American Association for the Advancement of Science
DOI: 10.1126/sciadv.adj0341