Descripción del proyecto
Diferencias basadas en el sexo en la inducción de muerte celular asociada con IRI
El daño por isquemia-reperfusión (IRI, por sus siglas en inglés) se produce durante la restauración del flujo sanguíneo tras un infarto de miocardio. La fisiopatología de la disfunción y muerte de los cardiomiocitos durante el IRI se ha estudiado profusamente, pero no se conoce bien la relevancia de las diferencias basadas en el sexo en este contexto. El objetivo del proyecto financiado con fondos europeos SEXOGENIRI es dilucidar el efecto del sexo biológico en la inducción de muerte celular durante el IRI. El proyecto examinará corazones humanos y de ratones mediante una serie de técnicas, que incluyen la cirugía «in vivo», la evaluación «in vitro» de las líneas celulares, microscopía de vanguardia, un estudio respiratorio mitocondrial y métodos tradicionales de biología molecular.
Objetivo
Acute myocardial infarction (MI) accounts for millions of morbidity and mortality cases worldwide. While the underlying pathophysiology of cardiomyocyte dysfunction and death during MI-related ischaemia/reperfusion injury (IRI), which occurs due to the restoration of coronary blood flow after MI, is well established, the relevance of sex-based differences in this context is poorly understood. In this action, we aim at understanding the effect of biological sex in the induction of a novel form of cell death in the setting of IRI. The project will examine both mouse and human hearts and will benefit from a range of techniques including, but not limited to in vivo surgery, in vitro cell line evaluation, state-of-the-art microscopy, mitochondrial respirometry and molecular biology methods. The content of the action will enable the researcher to restart his career and gain important academic, interpersonal and laboratory skills. It will also provide an opportunity for the researcher to publish in major journals and disseminate his findings to scientists and the public, which will ultimately help him establish himself in the field of cardiovascular science. The action also helps raise the research and academic profile of the host team and institution and contribute to the development of strong intra-European science networking. Overall, this action aims at contributing to the scientific progress of the researcher and pave the way for the identification of novel therapeutic targets for IRI with direct translational potential.
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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinador
101 Reykjavik
Islandia