Projektbeschreibung
Biofilme mit mehreren Arten in der Lebensmittelindustrie
Studien zu bakteriellen Biofilmen auf mit Lebensmitteln in Berührung kommenden Flächen in Lebensmittelindustrien haben die Verunreinigung durch viele bakterielle Spezies offenbart, auch schädliche und sporenformende Bakterien, die durch eine Reinigung nicht beseitigt werden. Das EU-finanzierte Projekt Multispeciesbiofilm wird die Fähigkeit dieser Spezies, Biofilme zu bilden, untersuchen. Dabei werden Interaktionen zwischen verschiedenen bakteriellen Gruppen in Biofilmen mit mehreren Arten analysiert, insbesondere der sporenformenden und nicht-sporenformenden Bakterien, wie Arten der Pseudomonas, Stenotrophomonas und Staphylokokkus. Das Ziel ist es, die Wirkung einer simulierten Reinigung und Desinfektion auf die Integrität dieser Biofilme auf Edelstahl in einem Biofilmmodell auf Basis eines Bioreaktors zu analysieren. Bei dieser multidisziplinären Initiative werden Metatranskriptomik, konfokale Laserrastermikroskopie und ein Biosensor eingesetzt, um das Verhalten von Bakterien in gemischten Biofilmgemeinschaften auf molekularer und struktureller Ebene zu untersuchen.
Ziel
Dairy industry is one of the largest food manufacturing sectors of Europe. Stainless steel is extensively used throughout the milk processing chain and is subjected to fouling leading to biofilm formation. Biofilms in the dairy industry pose a serious threat to the quality and safety of food products. Most studies on bacterial biofilms of the food industry have focused on single or dual species biofilms, negating the role of multispecies bacterial interaction, which is now considered to be a fundamental approach in studying biofilm community structure, spatial organization and factors underlying the biofilm formation on surfaces. Results of a recent project
show that the food contact surfaces in the dairy industry in Belgium are contaminated with many different bacterial species, including several spoilers and spore-forming bacteria, which remain largely unaffected even after cleaning-in-place (CIP). Biofilm forming capacity of many of these species and their interaction, particularly the interaction between sporeformers and other non spore-forming bacteria such as Pseudomonas, Stenotrophomonas and Staphylococcus will be investigated. The objective is to model multispecies biofilms in different species combinations and monitor their behaviour and influence on each other from initial adherence to biofilm formation on a steel surface under conditions simulating real manufacturing operations. We plan to study the influence of simulated cleaning and disinfection techniques on the integrity of single and multispecies biofilms on stainless steel in a bioreactor based biofilm model. This project is multidisciplinary involving meta-transcriptomics, confocal laser scanning microscopy and a biosensor. This proposal includes a strong linkage opportunity between the host and two secondment partners. Successful completion of this project will lead to more fundamental understanding of the formation of biofilms in food industrial practice.
Wissenschaftliches Gebiet
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsbiosensors
- engineering and technologyenvironmental biotechnologybioremediationbioreactors
- agricultural sciencesanimal and dairy sciencedairy
- engineering and technologyother engineering and technologiesfood technologyfood safety
- natural sciencesphysical sciencesopticsmicroscopyconfocal microscopy
Schlüsselbegriffe
Programm/Programme
Thema/Themen
Aufforderung zur Vorschlagseinreichung
Andere Projekte für diesen Aufruf anzeigenFinanzierungsplan
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Koordinator
9820 Merelbeke
Belgien