Descripción del proyecto
Información sobre la biología del glioblastoma
El glioblastoma multiforme (GBM) es un tumor encefálico de crecimiento rápido con un pronóstico desfavorable y una supervivencia escasa. Las opciones de tratamiento son limitadas y los tumores desarrollan resistencia a la radioterapia y la quimioterapia, lo que pone de manifiesto la importancia de un diagnóstico precoz y nuevos tratamientos. El objetivo del proyecto MAtChinG, financiado con fondos europeos, es ofrecer información fundamental sobre la biología y el microentorno del GBM. El trabajo se centrará en el mecanorreceptor principal PIEZO1, así como en la diafonía con la proteína de unión al ARN hnRNP K, que se sabe que desempeña un papel en la transcripción, la traducción y la estabilidad del ARN. Los investigadores estudiarán el vínculo de PIEZO1 y hnRNP K con las células madre de los gliomas, así como con la aparición y la malignidad del GBM.
Objetivo
Glioblastoma multiforme (GBM) is one of the most common and aggressive forms of brain tumour, affecting 2-3 per 100,000 adults per year, and with a usual survival time of 14-18 months after diagnosis with only a 10% of the patients living up to 5 years after it. Because of its location, early detection of the tumour and surgical removal may be complicated. Furthermore, its fast growth rate and migration capacity makes it extremely aggressive. Aditionally the presence of glioma cancer stem cells contribute to radio- and chemioresistance of GBM. Because of all this, a deeper understanding about its genetical, biochemical and microenviromental nature is needed. In order to gain more insight into the formation and development of glioma, in this proposed project we hypothesize that the heterogeneous nuclear ribonucleoprotein K (hnRNPK) and the mechanically activated ion channel Piezo1 constitute an axis that promotes the onset and progression of glioma. HnRNPK is a multifunctional protein that influences transcription, translation and RNA stability amongst other function, and it exerts its function depending on its phosphorylation state, which is modulated by kinases such as ERK, JNK or PKC. Piezo1 is a stretch-activated cation channel, that activated upon mechanical cues allowing the influx of calcium in the cell and activation of multiple cascades, such as ERK or PKC pathways, as well as in apoptosis and migration. Both hnRNPK and Piezo1 are upregulated in gliomas and preliminary data suggests a relation in their regulation. Here, we proposed that there is a regulation between Piezo1 and hnRNPK, and that this axis promotes the onset of gliomas cancer stem cells and contributes to its aggressiveness. With this project, we aim to investigate how this axis could contribute to the formation and aggressiveness of gliomas, and how its modulation can contribute to its control, therefore turning Piezo1 and hnRNPK into a new promising target for glioma treatment.
Ámbito científico
- natural scienceschemical sciencesinorganic chemistryalkaline earth metals
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteins
- medical and health sciencesmedical biotechnologycells technologiesstem cells
- medical and health sciencesclinical medicineoncology
- natural sciencesbiological sciencesgeneticsRNA
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Régimen de financiación
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinador
28029 Madrid
España