Descripción del proyecto
El papel de los parabióticos en la salud dental
Los parabióticos otorgan un conjunto de propiedades que fomentan la salud. Mientras que los parabióticos (en dosis apropiadas) han demostrado beneficios en diversos ámbitos médicos, todavía se deben probar en el campo de la salud bucodental. El proyecto INPAROL, financiado con fondos europeos, producirá parabióticos innovadores mediante tecnología de ultrasonido a partir de cepas mixtas de probióticos utilizados en productos comerciales. Se evaluarán los efectos de estos probióticos mediante pruebas antimicrobianas contra agentes patógenos bucales y en diversas condiciones medioambientales. Además, se someterá los parabióticos con un mejor rendimiento a pruebas de biopelículas, que replican las condiciones bucodentales, y a pruebas de toxicidad. El equipo de INPAROL supervisará los cambios moleculares ante mediadores inflamatorios con modelos de infección «in vitro» complejos.
Objetivo
Oral diseases constitute an increased major public health burden. Alternative dental prevention and therapeutic approaches such as probiotics are gaining popularity, but partly due to these products being live bacteria their use has not gained significant popularity in dentistry. Parabiotics in appropriate doses have been shown to induce health benefits in other medical fields but have only as yet been tested sparingly for dental use.
INPAROL aims at producing innovative parabiotics through ultrasound technology from mixed strains of probiotics used in commercial products. INPARAOL probiotics’ effects will be assessed by antimicrobial testing against oral pathogens (single and mixed culture) and also following exposure to varied environmental conditions. Best performing parabiotics will be subjected to biofilm assays replicating oral conditions and toxicity assays, in vitro cell monolayers and in vivo zebrafish embryo models, will be performed. Finally, the molecular changes of changes to inflammatory mediators will be monitored with complex in vitro infection models.
Overall, this is the first time parabiotics for oral health application will be produced with the proposed methods which makes this research so niche. My strong clinical and microbiology research background makes INPAROL an ideal fit. I will be joined by a strong supervisory team at the University of Malta consisting of a renowned microbiologist and co-supervised by a world-leader on innovative antimicrobial approaches from the Academic Medical Centre, Amsterdam. INPAROL affords an unprecedented opportunity to build on my expertise to kick-start an independent research path in oral health research.
Ámbito científico
Palabras clave
Programa(s)
Convocatoria de propuestas
Consulte otros proyectos de esta convocatoriaConvocatoria de subcontratación
H2020-WF-03-2020
Régimen de financiación
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinador
2080 MSIDA
Malta