In situ genetic perturbation of gut bacteria with engineered phage vectors and CRISPR

Project Information

crInSitu

Grant agreement ID: 101044479

DOI

10.3030/101044479

EC signature date 22 April 2022

Start date 1 September 2022

End date 31 August 2027

Funded under

• European Research Council (ERC)

Total cost

€ 1 999 780,00

EU contribution

€ 1 999 780,00

Coordinated by

INSTITUT PASTEUR
France

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Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (6)

Computational design of novel Cas9 PAM-interacting domains using evolution-based modelling and structural quality assessment

Author(s): Cyril Malbranke; William Rostain; Florence Depardieu; Simona Cocco; Rémi Monasson; David Bikard
Published in: PLoS Computational Biology, Vol 19, Iss 11, p e1011621 (2023), 2023, ISSN 1553-7358
Publisher: PLoS Computational Biology
DOI: 10.1371/journal.pcbi.1011621

Generation of a Biotin-Tagged Dual-Display Phage

Author(s): De Plano, Laura Maria; Oddo, Salvatore; Bikard, David; Caccamo, Antonella; Conoci, Sabrina
Published in: Cells, 2024, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/CELLS13201696

Nucleic Acids Research

Author(s): William Rostain; Theophile Grebert; Danylo Vyhovskyi; Paula Thiel Pizarro; Gatwa Tshinsele-Van Bellingen; Lun Cui; David Bikard
Published in: "Nucleic Acids Research, 2023, 51 (7), pp.3485-3496. ⟨10.1093/nar/gkad170⟩", 2023, ISSN 0305-1048
Publisher: Nucleic Acids Research
DOI: 10.1093/nar/gkad170

A virally encoded tRNA neutralizes the PARIS antiviral defence system

Author(s): Nathaniel Burman, Svetlana Belukhina, Florence Depardieu, Royce A. Wilkinson, Mikhail Skutel, Andrew Santiago-Frangos, Ava B. Graham, Alexei Livenskyi, Anna Chechenina, Natalia Morozova, Trevor Zahl, William S. Henriques, Murat Buyukyoruk, Christophe Rouillon, Baptiste Saudemont, Lena Shyrokova, Tatsuaki Kurata, Vasili Hauryliuk, Konstantin Severinov, Justine Groseille, Agnès Thierry, Romain Koszul, Florian Tesson, Aude Bernheim, David Bikard, Blake Wiedenheft, Artem Isaev
Published in: Nature, Issue 634, 2024, ISSN 0028-0836
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s41586-024-07874-3

In situ targeted base editing of bacteria in the mouse gut

Author(s): Andreas K. Brödel; Loïc H. Charpenay; Matthieu Galtier; Fabien J. Fuche; Rémi Terrasse; Chloé Poquet; Jan Havránek; Simone Pignotti; Antonina Krawczyk; Marion Arraou; Gautier Prevot; Dalila Spadoni; Matthew T. N. Yarnall; Edith M. Hessel; Jesus Fernandez-Rodriguez; Xavier Duportet; David Bikard
Published in: Nature, 2024, ISSN 1476-4687
Publisher: Nature
DOI: 10.1038/s41586-024-07681-w

Delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria through a type IV secretion system

Author(s): Dolores L. Guzmán-Herrador; Andrea Fernández-Gómez; Florence Depardieu; David Bikard; Matxalen Llosa
Published in: Proceedings of the National Academy of Sciences, 2024, ISSN 1091-6490
Publisher: Proceedings of the National Academy of Science
DOI: 10.1073/PNAS.2408509121