Periodic Reporting for period 1 - MICROBE (ModulatIng Cancer therapy RespOnse using Bacterial Extracellular nanovesicles)
Berichtszeitraum: 2022-11-01 bis 2025-04-30
Bacterial extracellular vesicles against cancer
Like eukaryotic cells, bacteria secrete nanometre-sized extracellular vesicles (BEVs) loaded with active cargo such as proteins as a mechanism of communication with other bacteria and with the host. Scientists under the EU-funded MICROBE project had previously discovered that gut microbiota also secrete BEVs in the systemic circulation of patients with increased intestinal permeability. The biological significance of BEVs in the interplay between the host and bacteria is still largely unknown. Through in-depth characterisation of BEVs from healthy individuals and patients with increased intestinal permeability, MICROBE aims to investigate how BEVs influence the disease course.
Like eukaryotic cells, bacteria secrete nanometre-sized extracellular vesicles (BEVs) loaded with active cargo such as proteins as a mechanism of communication with other bacteria and with the host. Scientists under the EU-funded MICROBE project had previously discovered that gut microbiota also secrete BEVs in the systemic circulation of patients with increased intestinal permeability. The biological significance of BEVs in the interplay between the host and bacteria is still largely unknown. Through in-depth characterisation of BEVs from healthy individuals and patients with increased intestinal permeability, MICROBE aims to investigate how BEVs influence the disease course.
1) A high-quality biorepository of healthy individuals and patients with increased intestinal permeability has been completed.
2) Current practices in BEV research have been mapped. We analyzed 845 publications released in 2015-2021, reporting 3338 BEV-related experiments. The extracted data are accessible via the publicly available EV-TRACK knowledgebase ( https://evtrack.org/(öffnet in neuem Fenster)). We identify the need for transparent reporting, delineate knowledge gaps, outline available best practices and define areas in need of guidance to ensure advances in BEV research and accelerate BEV applications.
3) The workflow for BEV preparation from these biofluids has been successfully fine-tuned.
4) BEV have been prepared from the collected biofluids and omics analysis and functional experiments are currently ongoing.
2) Current practices in BEV research have been mapped. We analyzed 845 publications released in 2015-2021, reporting 3338 BEV-related experiments. The extracted data are accessible via the publicly available EV-TRACK knowledgebase ( https://evtrack.org/(öffnet in neuem Fenster)). We identify the need for transparent reporting, delineate knowledge gaps, outline available best practices and define areas in need of guidance to ensure advances in BEV research and accelerate BEV applications.
3) The workflow for BEV preparation from these biofluids has been successfully fine-tuned.
4) BEV have been prepared from the collected biofluids and omics analysis and functional experiments are currently ongoing.
The project has already delivered recommendations and identified a set of experimental parameters, to delineate the available guidelines and tools with particular needs for BEV research. Results obtained from ongoing omics analysis and functional experiments are currently being explored for IP development.