RNA PROCESSING FOR ANTI-CANCER IMMUNOTHERAPY

Project Information

CANCERNA

Grant agreement ID: 101057250

DOI

10.3030/101057250

Project closed

EC signature date 19 May 2022

Start date 1 June 2022

End date 31 May 2025

Funded under

• Health

Total cost

€ 5 999 381,25

EU contribution

€ 5 999 381,00

Coordinated by

HADASSAH MEDICAL ORGANIZATION
Israel

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Data sets, microdata, etc (2)

List of altered transcripts and NMD-prone transcripts

a. List of altered transcripts, which are induced by the presence of splicing factor mutations and generate predicted immunogenic peptides (M18)b.List of NMD-prone transcripts which are induced by the presence of NMD inhibitors generate predicted immunogenic peptides (M18)

Bio-bank of tumour samples and generation of RNA-seq datasets

a. Bio-bank of tumour samples (uveal melanoma and MDS/AML) harbouring mutations in splicing factor genes (M12)b.Generation of RNA-seq datasets and splicing analysis of tumour samples (uveal melanoma and MDS/AML) harbouring mutations in splicing factor genes (M18)

Websites, patent fillings, videos etc. (1)

CANCERNA project website and social media platforms

Other (1)

Selection of a reproducible method to be adopted by participating groups, of mRNA-loaded APC preparation for vaccine epitope presentation.

Publications

Peer reviewed articles (16)

The future of affordable cancer immunotherapy

Author(s): Schaft N, Dörrie J, Schuler G, Schuler-Thurner B, Sallam H, Klein S, Eisenberg G, Frankenburg S, Lotem M, Khatib A.
Published in: Frontiers in Immunology, Issue 1248867, 2023, ISSN 1664-3224
Publisher: Frontiers Media SA
DOI: 10.3389/fimmu.2023.1248867

Tumor Antigens beyond the Human Exome

Author(s): Lisabeth Emilius, Franziska Bremm, Amanda Katharina Binder, Niels Schaft, Jan Dörrie
Published in: International Journal of Molecular Sciences, Issue 25, 2025, ISSN 1422-0067
Publisher: MDPI AG
DOI: 10.3390/IJMS25094673

Cancers

Author(s): S. Matos, P. Bernardo, S. Esteves, A.B.d. Sousa, M. Lemos, P. Ribeiro, M. Silva, A. Nunes, J. Lobato, M.d.J. Frade, M.G.da. S, S. Chacim, J. Mariz, G. Esteves,J. Raposo, A. Espadana, J. Carda, P. Barbosa, V. Martins, M. Carmo-Fonseca, J. Desterro
Published in: Cancers, Issue 14133236, 2022, ISSN 2072-6694
Publisher: MDPI
DOI: 10.3390/cancers14133236

Spliced to kill: RNA mis-splicing derived cancer neoantigens

Author(s): Anurag V. Prabhu, Carla Azar-Koussa, Zahava Siegfried, Rotem Karni
Published in: Trends in Cancer, 2025, ISSN 2405-8033
Publisher: Elsevier BV
DOI: 10.1016/J.TRECAN.2025.06.001

Targeting splicing factors for cancer therapy

Author(s): Ariel Bashari, Zahava Siegfried, Rotem Karni
Published in: RNA, Issue 29, 2023, ISSN 1355-8382
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1261/RNA.079585.123

Gene network-based and ensemble modeling-based selection of tumor-associated antigens with a predicted low risk of tissue damage for targeted immunotherapy

Author(s): Christopher Lischer, Martin Eberhardt, Cindy Flamann, Johannes Berges, Esther Güse, Anja Wessely, Adrian Weich, Jimmy Retzlaff, Jan Dörrie, Niels Schaft, Manuel Wiesinger, Johannes März, Beatrice Schuler-Thurner, Harald Knorr, Shailendra Gupta, Krishna Pal Singh, Gerold Schuler, Markus Vincent Heppt, Elias Andreas Thomas Koch, Nadine D van Kleef, Julian J Freen-van Heeren, Annelies W Turksma, Olaf Wolkenhauer, Bettina Hohberger, Carola Berking, Heiko Bruns, Julio Vera
Published in: Journal for ImmunoTherapy of Cancer, Issue 12, 2024, ISSN 2051-1426
Publisher: BMJ
DOI: 10.1136/JITC-2023-008104

Generation of tumor neoantigens by RNA splicing perturbation

Author(s): Adi Rosenberg-Mogilevsky, Zahava Siegfried, Rotem Karni
Published in: Trends in Cancer, Issue 11, 2025, ISSN 2405-8033
Publisher: Elsevier BV
DOI: 10.1016/J.TRECAN.2024.10.008

Non-Coding RNA in Tumor Cells and Tumor-Associated Myeloid Cells—Function and Therapeutic Potential

Author(s): Amanda Katharina Binder, Franziska Bremm, Jan Dörrie, Niels Schaft
Published in: International Journal of Molecular Sciences, Issue 25, 2025, ISSN 1422-0067
Publisher: MDPI AG
DOI: 10.3390/IJMS25137275

RBFOX2 modulates a metastatic signature of alternative splicing in pancreatic cancer

Author(s): Amina Jbara, Kuan-Ting Lin, Chani Stossel, Zahava Siegfried, Haya Shqerat, Adi Amar-Schwartz, Ela Elyada, Maxim Mogilevsky, Maria Raitses-Gurevich, Jared L. Johnson, Tomer M. Yaron, Ofek Ovadia, Gun Ho Jang, Miri Danan-Gotthold, Lewis C. Cantley, Erez Y. Levanon, Steven Gallinger, Adrian R. Krainer, Talia Golan, Rotem Karni
Published in: Nature, Issue 617, 2023, ISSN 0028-0836
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41586-023-05820-3

Apolipoprotein E4 facilitates transfection of human monocyte-derived dendritic cells by lipid nanoparticles

Author(s): Izabella Lambart, Hannah Zaryouh, Jonas Van Audenaerde, Dana Liu, Delphine Quatannens, Eva Lion, Stefan Schiller, Simon Geissler, Evelien Smits, Karsten Mäder
Published in: International Journal of Pharmaceutics, Issue 678, 2025, ISSN 0378-5173
Publisher: Elsevier BV
DOI: 10.1016/J.IJPHARM.2025.125720

Precise in vivo RNA base editing with a wobble-enhanced circular CLUSTER guide RNA

Author(s): Philipp Reautschnig, Carolin Fruhner, Nicolai Wahn, Charlotte P. Wiegand, Sabrina Kragness, John F. Yung, Daniel T. Hofacker, Jenna Fisk, Michelle Eidelman, Nils Waffenschmidt, Maximilian Feige, Laura S. Pfeiffer, Annika E. Schulz, Yvonne Füll, Erez Y. Levanon, Gail Mandel, Thorsten Stafforst
Published in: Nature Biotechnology, Issue 43, 2025, ISSN 1087-0156
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41587-024-02313-0

Melanoma 2.0. Skin cancer as a paradigm for emerging diagnostic technologies, computational modelling and artificial intelligence

Author(s): Julio Vera, Xin Lai, Andreas Baur, Michael Erdmann, Shailendra Gupta, Cristiano Guttà, Lucie Heinzerling, Markus V Heppt, Philipp Maximilian Kazmierczak, Manfred Kunz, Christopher Lischer, Brigitte M Pützer, Markus Rehm, Christian Ostalecki, Jimmy Retzlaff, Stephan Witt, Olaf Wolkenhauer, Carola Berking
Published in: Briefings in Bioinformatics, Issue 23, 2022, ISSN 1467-5463
Publisher: Oxford University Press (OUP)
DOI: 10.1093/BIB/BBAC433

The Influence of Indisulam on Human Immune Effector Cells: Is a Combination with Immunotherapy Feasible?

Author(s): Lisa Arnet, Lisabeth Emilius, Annett Hamann, Maria Carmo-Fonseca, Carola Berking, Jan Dörrie, Niels Schaft
Published in: Pharmaceutics, Issue 17, 2025, ISSN 1999-4923
Publisher: MDPI AG
DOI: 10.3390/PHARMACEUTICS17030368

Alternative splicing of modulatory immune receptors in T lymphocytes: a newly identified and targetable mechanism for anticancer immunotherapy

Author(s): Shay Tzaban, Ori Stern, Elad Zisman, Galit Eisenberg, Shiri Klein, Shoshana Frankenburg, Michal Lotem
Published in: Frontiers in Immunology, Issue 15, 2025, ISSN 1664-3224
Publisher: Frontiers Media SA
DOI: 10.3389/FIMMU.2024.1490035

Unveiling alternative splicing dynamics in activated T lymphocytes and their implications for immune checkpoint blockade efficacy

Author(s): Elad Zisman, Ori Stern, Reyut Lewis, Shay Tzaban 1, Thomas Eisenhaure, Sarah E Henrickson, Moshe Sade-Feldman, Shira Silberman-Klein, Galit Eisenberg, Nir Hacohen, Keren Yizhak, Michal Lotem
Published in: iScience, 2025, ISSN 2589-0042
Publisher: Cell Press
DOI: 10.1016/J.ISCI.2025

Transcriptome-wide splicing network reveals specialized regulatory functions of the core spliceosome

Author(s): Malgorzata E. Rogalska, Estefania Mancini, Sophie Bonnal, André Gohr, Bryan M. Dunyak, Niccolò Arecco, Peter G. Smith, Frédéric H. Vaillancourt, Juan Valcárcel
Published in: Science, Issue 386, 2024, ISSN 0036-8075
Publisher: American Association for the Advancement of Science (AAAS)
DOI: 10.1126/SCIENCE.ADN8105

Conference proceedings (1)

Human Gene Therapy

Author(s): S. Tzaban, P. Appasamy, E. Abdallah, R. Lewis, E. Zisman, S. Klein, R. Karni, G. Eisenberg, M. Lotem
Published in: International Conference on Lymphocyte Engineering, Issue 4, 2023, ISSN 1043-0342
Publisher: Human Gene Therapy
DOI: 10.1089/hum.2023.29253.abstracts.index