CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.
I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .
Risultati finali
(T3.3)Report on a selection of genomically divergent varieties (established in WP4). Grain δ13C-carbon isotope discrimination will be measured to determine water use efficiency. For selected, contrasting genotypes, the (deep) soil C sequestration potentials will be determined by relating experimentally derived decomposition rates of roots and residue (in litter bags) at different soil depths to (root) biomass depth distributions.
Report on soil analysis and root distribution, biomass and morphological traits (si apre in una nuova finestra)(T3.3)Report on root phenotypes and soil analysis iin field trials. Belowground samples will be taken in the same area used for the aboveground sampling using manual or tractor mounted drills at depths of up to 1m. At BOKU, fluorescent imaging on soil cores (core break method, 20 cm resolution) will be used to determine root biomass depth distribution and at both ICARDA and BOKU the soil samples at selected depth horizons will be rinsed to determine root biomass and morphological traits. These data will be used by BOKU to be correlated to root biomass per depth (of barley and weeds, if present) and established models will be validated on a subset of contrasting genotypes.
List of members of Steering board and advisory board (si apre in una nuova finestra)(T1.1). At the Kick-off meeting in month 1 we will establish the Steering Board, with a representative member from each partner, and the Advisory Board.
First report on genome editing outlining specific gene knockout and transgenic barley varieties (si apre in una nuova finestra)(T7.2-T7.4)Genome editing of selected barley varieties by non-homologous end-joining (NHEJ), homology directed repair (HDR) or base editing. We will use gene knock-out by mainly CRISPR/Cas or alternatively TALEN induced NHEJ. NHEJ typically leads to indels and introduction of small deletions/insertions at the site of break, resulting in knockout of gene function via frame shift mutations. Genome editing constructs for sim- and multiplex gene editing will be generated and introduced as described in task 7.1. To fixate the mutations in the barley genome, the primary mutants will be self-pollinated and genotyped in the next generation. After genotyping, plants where the genome editing construct has been segregated away will be identified. Similarly, barley genome editing will be performed by gene or motif replacement via HDR. In BarleyMicroBreed, HDR is expected to be used for induction of expression i.e. tandem repeats in promoters or change of transcription factor binding motifs. We aim for using an improved technology where the repair template flanked by the CRISPR/Cas recognition motifs is delivered together with the nuclease. After transformation, the CRISPR/Cas introduce double strand breaks at the endogenous locus. To facilitate a broader target area in the genome, the CRISPR/Cas component can be replaced by a TALEN.In special cases for answering specific research questions, we will use base editors. Base editing will be used to mutate single nucleotides in a predetermined region of the barley genome. Hereby specific single nucleotide polymorphism in a barley variety can be created that potentially can modulate transcription factor binding sites and gene transcription levels. Gene constructs holding the base editors will be introduced as described under the previous tasks. To fixate the mutations in the genome, the plants will be self-pollinated and genotyped.
Update of plan for dissemination and exploitation including communication activities (si apre in una nuova finestra)(T2.1)The plan for dissemination and exploitation including communication activities will be reviewed and updated to appropriately reflect any changes since implementation.
Update of DMS (si apre in una nuova finestra)(T1.4)Report on update of DMS
Metagenomics data report on shot-gun sequencing of microbiota from a selection of barley genotypes (si apre in una nuova finestra)(T5.3)Report on metagenome sequencing from rhizosphere samples from a selection of the barley genotypes. Metagenomic sequences will be assembled and genes will be predicted and annotated using ab initio, reference-based and metatranscriptome-based approaches. Sequences from the metatranscriptome libraries will be mapped to annotated gene sequences available in public databases (e.g. UniProt, Ensembl, RefSeq and EMG).
Report on precision phenotyping using PhysioTron (si apre in una nuova finestra)(T3.5)Report on PhysioTron phenotyping. A subset of 50-70 genotypes from each panel will be selected based on the field trials’ results (Task 3.3) to undergo precision phenotyping under well-watered and drought conditions using the PhysioTron, a weighing lysimeter built by ICARDA in the HEAT&DROUGHT research platform of Settat (Morocco).
Plan for dissemination and exploitation including communication activities (si apre in una nuova finestra)(T2.1)AU will lead an analysis with input from all partners to identify expected results that will have high scientific, industrial and societal impact. For each identified result, we will define relevant target groups, specifying concrete institutions, companies or individuals, where relevant. Further, we will identify the most appropriate means and concrete channels of dissemination/communication. We will schedule concrete dissemination/communication activities and the responsible partners. AU will, in close collaboration with all partners, particularly commercial partners, analyse which expected results will have commercial potential. Based on this, we will construct an exploitation plan scheduling when to assess the potential exploitation of specific inventions and an action plan, which will define i) criteria for exploitation potential; ii) criteria for seeking expert advice on IPR; iii) relevant IPR bodies to contact. The combined PDEC will be completed by month 6 but will be sufficiently flexible to accommodate new opportunities for improved dissemination, exploitation and communication arising during the lifespan of the project.
DNA extraction (si apre in una nuova finestra)(T5.1) DNA from all barley varieties from Task 3.3 will be extracted from rhizosphere soil samples
Data Management System - DMS (si apre in una nuova finestra)(T1.4)The SMs will be in charge of integrating the Data Management System, namely a SQL database with user interface, to the project Intranet, and will also create a user guide by Month 12.
(T1.4)DMP will be reviewed and updated to appropriately reflect any changes since implementation.
Data Management Plan - DMP (si apre in una nuova finestra)(T1.4)A Data Management Plan (DMP) will be created with the aim to maximise access to and re-use of research data produced in BarleyMicroBreed, in line with the FAIR principles. SMs will integrate the data produced in BarleyMicroBreed WPs and generate an interface to connect all the data and results generated in the project providing a user-friendly environment. We will make sure metadata for all the field and laboratory trials performed in this project will be correctly retrieved and stored in databases. The project data and results will be stored and shared in open-access repositories and publications.
(T3.1)Three panels of 200 barley genotypes each will be selected based on genetic diversity, growth type (spring and winter) and geographical representation with emphasis on Europe and the Mediterranean Basin. The first panel will consist of a high diversity assembly of landrace- and barley wild relative-derived genotypes developed by ICARDA for which previous knowledge of phenotypic variability for abiotic stress tolerance is available. The second and third panel will consist of mostly European and Mediterranean Basin cultivars representative of those grown in the region assembled in growth type-specific panels. All accessions are available as multiplied seed stocks from ICARDA and will be accessible through the project website (www.barleymicrobreed.eu).
www.barleymicrobreed.eu website (si apre in una nuova finestra)(T2.2)AU will be in charge of creating and managing the public www.barleymicrobreed.eu website , which will centralise all the VCS. AU will also create and manage Facebook, Twitter, Instagram, LinkedIn and YouTube accounts that will be integrated on the website. Articles related to BarleyMicroBreed will be posted every month, including interviews to project partners, and these posts will be broadcasted in social media.
Pubblicazioni
Autori:
Katarzyna Retzer; Verena Ibl
Pubblicato in:
Frontiers in Plant Science, 2023, ISSN 1664-462X
Editore:
Frontiers Media SA
DOI:
10.3389/fpls.2023.1227490
Autori:
Jovana Eskildsen, Menghui Dong, Tobias Hanak, Claus Krogh Madsen, Inger Holme, Tamás Plaszkó, Mette Vestergård, Mogens Nicolaisen, Hans Thordal-Christensen, Henrik Brinch-Pedersen
Pubblicato in:
International Journal of Molecular Sciences, Numero 27, 2026, ISSN 1422-0067
Editore:
MDPI AG
DOI:
10.3390/IJMS27041846
Autori:
Somayeh Gholizadeh; Iman Nemati; Mette Vestergård; Christopher James Barnes; Enoch Narh Kudjordjie; Mogens Nicolaisen
Pubblicato in:
Microbiological Research, 2024, ISSN 1618-0623
Editore:
Elsevier
DOI:
10.1016/J.MICRES.2024.127698
Autori:
Tobias Hanak; Jovana Jancic; Fiona R. Hay; Henrik Brinch-Pedersen
Pubblicato in:
Frontiers in Sustainable Food Systems, Numero 7, 2023, ISSN 2571-581X
Editore:
FRONTIERS
DOI:
10.3389/fsufs.2023.1331577
Autori:
Hanak, Tobias; Andrzejczak, Olga A.; Hebelstrup, Kim; Brinch-Pedersen, Henrik
Pubblicato in:
Plant Physiology and Biochemistry, 2024, ISSN 0981-9428
Editore:
Elsevier
DOI:
10.1016/j.plaphy.2024.109174
Autori:
Michael Panting, Inger B. Holme, Giuseppe Dionisio, Henrik Brinch-Pedersen
Pubblicato in:
Plant Biotechnology Journal, 2025, ISSN 1467-7652
Editore:
Wiley
DOI:
10.1111/pbi.70065
Autori:
Pree, Simon; Malekian, Babak; Sandén, Hans; Nicolaisen, Mogens; Weckwerth, Wolfram; Madsen, Mette Vestergård; Retzer, Katarzyna
Pubblicato in:
Frontiers in Plant Physiology, 2023, ISSN 2813-821X
Editore:
Frontiers Media
DOI:
10.3389/FPHGY.2023.1308534
Autori:
Simon Pree; Ivan Kashkan; Katarzyna Retzer
Pubblicato in:
bioRxiv, 2024, ISSN 2692-8205
Editore:
bioRxiv
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