Towards a Mechanistic Understanding of Nanoparticle Interactions with Exosome Secretion

Información del proyecto

NanoEXOS

Identificador del acuerdo de subvención: 101063905

DOI

10.3030/101063905

Proyecto cerrado

Fecha de la firma de la CE 3 Agosto 2022

Fecha de inicio 1 Septiembre 2023

Fecha de finalización 31 Agosto 2025

Financiado con arreglo a

• Marie Skłodowska-Curie Actions (MSCA)

Coste total

Sin datos

Aportación de la UE

€ 199 694,40

Coordinado por

UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN
Ireland

Objetivo

In recent decades, considerable progress has been made in bio-nano research towards a growing understanding of the interaction mechanisms between nanoparticles (NPs) and living systems. Yet, the impact that cell-internalised NPs have on exosome secretion remains largely unknown. Exosomes are ubiquitous cell-released vesicles seemingly involved in numerous biological processes, including regulation of cell-cell communication and disease progression. This enigmatic functional complexity is under the spotlight of a large scientific community in the fields of chemistry, biology and nanomedicine.
Only recently, it has been reported that in vitro cellular uptake of platinum and iron oxide NP clusters (~ 100 nm in size) significantly increases the release of exosomes and alters their composition. However, it is unclear whether this evidence reflects a general behaviour or depends on the design of the NPs. A strong need thus arises to explore the mechanistic details of this interaction. Inspired by this challenge, NanoEXOS aims to develop a novel framework to obtain a better and more general understanding of how engineered NPs interfere with the regulatory processes of exosome formation and release. NP-cell studies will be undertaken in vitro to focus on the effects that NPs have on cell-specific exosome samples. Importantly, NanoEXOS will systematically investigate how this interaction depends on the properties of NPs (e.g. well-defined size, shape, surface modification), their biomolecular interactions and their cellular entry mechanism.
Overall, NanoEXOS will contribute to expanding our knowledge of how exogenous NPs are processed by living systems. A thorough comprehension of how NPs access and alter key cellular machineries, such as exosome secretion, plays a pivotal role in the biological evaluation of these nanomaterials. This research question is crucial to establish safe and robust biological control over advanced NP-assisted diagnostic and therapeutic strategies.

Ámbito científico (EuroSciVoc)

• ciencias agrícolas › agricultura, silvicultura y pesca › agricultura › agronomía › protección fitosanitaria
• ciencias naturales › ciencias químicas › química inorgánica › metales de transición
• ciencias naturales › ciencias biológicas › biología celular
• ciencias médicas y de la salud › biotecnología médica › nanomedicina
• ingeniería y tecnología › nanotecnología › nanomateriales

Palabras clave

• nanoparticles
• cell cultures
• nanoparticle-cell studies
• intracellular nanoparticle trafficking
• cell imaging
• endosomal pathway
• exosome biogenesis
• exosome analysis
• nanosafety
• nanomedicine

Programa(s)

• HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) PROGRAMA PRINCIPAL
  

Tema(s)

• HORIZON-MSCA-2021-PF-01-01 - MSCA Postdoctoral Fellowships 2021
  

Régimen de financiación

HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

Convocatoria de propuestas

HORIZON-MSCA-2021-PF-01

Coordinador

UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN

• Aportación neta de la UEn: € 199 694,40
• Dirección: BELFIELD ; 4 DUBLIN ; Irlanda
• Tipo de actividad: Higher or Secondary Education Establishments
• Coste total: Sin datos