Objectif In recent decades, considerable progress has been made in bio-nano research towards a growing understanding of the interaction mechanisms between nanoparticles (NPs) and living systems. Yet, the impact that cell-internalised NPs have on exosome secretion remains largely unknown. Exosomes are ubiquitous cell-released vesicles seemingly involved in numerous biological processes, including regulation of cell-cell communication and disease progression. This enigmatic functional complexity is under the spotlight of a large scientific community in the fields of chemistry, biology and nanomedicine.Only recently, it has been reported that in vitro cellular uptake of platinum and iron oxide NP clusters (~ 100 nm in size) significantly increases the release of exosomes and alters their composition. However, it is unclear whether this evidence reflects a general behaviour or depends on the design of the NPs. A strong need thus arises to explore the mechanistic details of this interaction. Inspired by this challenge, NanoEXOS aims to develop a novel framework to obtain a better and more general understanding of how engineered NPs interfere with the regulatory processes of exosome formation and release. NP-cell studies will be undertaken in vitro to focus on the effects that NPs have on cell-specific exosome samples. Importantly, NanoEXOS will systematically investigate how this interaction depends on the properties of NPs (e.g. well-defined size, shape, surface modification), their biomolecular interactions and their cellular entry mechanism.Overall, NanoEXOS will contribute to expanding our knowledge of how exogenous NPs are processed by living systems. A thorough comprehension of how NPs access and alter key cellular machineries, such as exosome secretion, plays a pivotal role in the biological evaluation of these nanomaterials. This research question is crucial to establish safe and robust biological control over advanced NP-assisted diagnostic and therapeutic strategies. Champ scientifique sciences naturellessciences chimiqueschimie inorganiquemétal de transitionsciences naturellessciences biologiquesbiologie cellulairesciences médicales et de la santébiotechnologie médicalenanomédecineingénierie et technologienanotechnologienanomatériaux Programme(s) HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme Thème(s) HORIZON-MSCA-2021-PF-01-01 - MSCA Postdoctoral Fellowships 2021 Appel à propositions HORIZON-MSCA-2021-PF-01 Voir d’autres projets de cet appel Régime de financement HORIZON-AG-UN - HORIZON Unit Grant Coordinateur UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN Contribution nette de l'UE € 199 694,40 Adresse Belfield 4 Dublin Irlande Voir sur la carte Région Ireland Eastern and Midland Dublin Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Contribution de l’UE Aucune donnée
