Doctoral Network for a Shared Excellence of Fluorescent Lifetime Imaging Microscopy in Biomedical Applications

Project Information

flIMAGIN3D

Grant agreement ID: 101073507

DOI

10.3030/101073507

EC signature date 15 July 2022

Start date 1 January 2023

End date 31 December 2026

Funded under

• Marie Skłodowska-Curie Actions (MSCA)

Total cost

No data

EU contribution

€ 2 743 459,20

Coordinated by

THE PROVOST, FELLOWS, FOUNDATION SCHOLARS & THE OTHER MEMBERS OF BOARD, OF THE COLLEGE OF THE HOLY & UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN
Ireland

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Websites, patent fillings, videos etc. (3)

Publication of recruitment call

Call for DC applications announced via all supervisor channels, EURAXXAS portal

Updated flIMAGIN3D website/ social media accounts are live

Virtual portal is live for partners, and public website of the consortium

Link(s) to EURAXESS advertisement(s) of the positions

Documents, reports (1)

Secondment Plan

Secondment Plan (M12)

Other (1)

Banners, flyers and posters are available

All partners have acesss to all media files, logos, posters and promotional and dissemination level templates related to flIMAGIN3D.

Publications

Peer reviewed articles (21)

Production and Multi-Parameter Live Cell Fluorescence Lifetime Imaging Microscopy (FLIM) of Multicellular Spheroids

Author(s): Angela C. Debruyne, Gabriele Ferrari, Hang Zhou, Nore Van Loon, Nina Heymans, Irina A. Okkelman, Ruslan I. Dmitriev
Published in: Journal of Visualized Experiments, 2024, ISSN 1940-087X
Publisher: MyJove Corporation
DOI: 10.3791/66845

2P-FLIM unveils time-dependent metabolic shifts during osteogenic differentiation with a key role of lactate to fuel osteogenesis via glutaminolysis identified

Author(s): Nuno G. B. Neto, Meenakshi Suku, David A. Hoey & Michael G. Monaghan
Published in: Stem Cell Research & Therapy, Issue 364, 2023
Publisher: BMC
DOI: 10.1186/s13287-023-03606-y

Description of Pseudomonas imrae sp. nov., carrying a novel class C β-lactamase gene variant, isolated from gut samples of Atlantic mackerel (Scomber scombrus)

Author(s): Francisco Salvà-Serra; Francisco Salvà-Serra; Francisco Salvà-Serra; Francisco Salvà-Serra; Francisco Salvà-Serra; Priyank Nimje; Beatriz Piñeiro-Iglesias; Beatriz Piñeiro-Iglesias; Leonarda Achá Alarcón; Leonarda Achá Alarcón; Leonarda Achá Alarcón; Sofia Cardew; Sofia Cardew; Elisabeth Inganäs; Elisabeth Inganäs; Susanne Jensie-Markopoulos; Susanne Jensie-Markopoulos; Maria Ohlén; Maria Ohlén; Hanna-Sophia Sailer; Hanna-Sophia Sailer; Christel Unosson; Christel Unosson; Víctor Fernández-Juárez; Cesar O. Pacherres; Michael Kühl; Edward R. B. Moore; Edward R. B. Moore; Edward R. B. Moore; Edward R. B. Moore; Nachiket P. Marathe
Published in: Frontiers in Microbiology, 2025
Publisher: FRONTIERS
DOI: 10.3389/FMICB.2025.1530878

Engineering macrophage responses through 3D scaffold microarchitecture

Author(s): Chiara Martinelli, Srijan Chakraborty, Giovanni Buccioli, Matteo Vicini, Claudio Conci, Giulio Cerullo, Roberto Osellame, Giuseppe Chirico, Emanuela Jacchetti, Manuela Teresa Raimondi
Published in: Materials Today Bio, Issue 35, 2025, ISSN 2590-0064
Publisher: Elsevier BV
DOI: 10.1016/J.MTBIO.2025.102328

In-vivo Lifetime Imaging of the Internal O2 Dynamics in Corals with NIR-emitting Sensor Nanoparticles

Author(s): Michael Kühl; Daniel Aagren Nielsen; Sergey M. Borisov
Published in: Crossref, 2024
Publisher: CHERMXIV
DOI: 10.26434/CHEMRXIV-2024-M6KJ8

Oxygen Supply of Islets of Langerhans by Photosynthetically Active Microalgae in Bioprinted Co‐Cultures Maintains Their Function in a Hypoxic Environment

Author(s): Finn Dani, Sarah Duin, Ashwini Rahul Akkineni, Susann Lehmann, Barbara Ludwig, Michael Kühl, Michael Gelinsky, Anja Lode
Published in: Advanced Healthcare Materials, 2026, ISSN 2192-2640
Publisher: Wiley
DOI: 10.1002/ADHM.202505927

Functional imaging of 3D bioprinted microalgal constructs and simulation of their photosynthetic performance

Author(s): Swathi Murthy, Maria Mosshammer, Erik Trampe, Michael Kühl
Published in: Biofabrication, Issue 17, 2025, ISSN 1758-5082
Publisher: IOP Publishing
DOI: 10.1088/1758-5090/ADF9CA

Visualizing the internalization and biological impact of nanoplastics in live intestinal organoids by Fluorescence Lifetime Imaging Microscopy (FLIM)

Author(s): Irina A. Okkelman, Hang Zhou, Sergey M. Borisov, Angela C. Debruyne, Austin E. Y. T. Lefebvre, Marcelo Leomil Zoccoler, Linglong Chen, Bert Devriendt, Ruslan I. Dmitriev
Published in: Light: Science & Applications, Issue 14, 2025, ISSN 2047-7538
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41377-025-01949-0

Cytosolic‐enhanced dark Epac‐based <scp>FRET</scp> sensors allow for intracellular <scp>cAMP</scp> detection in live cells via <scp>FLIM</scp>

Author(s): Giulia Zanetti, Jeffrey B. Klarenbeek, Kees Jalink
Published in: FEBS Letters, 2024, ISSN 0014-5793
Publisher: Wiley
DOI: 10.1002/1873-3468.15093

Advanced healthcare materials

Author(s): Finn Dani, Sarah Duin, Ashwini Rahul Akkineni, Susann Lehmann, Barbara Ludwig, Michael Kühl, Michael Gelinsky, Anja Lode
Published in: Advanced Healthcare Materials, ISSN 2192-2640
Publisher: Advanced Healthcare Materials
DOI: 10.1002/ADHM.202505927DIGITALOBJECTIDENTIFIER(DOI)

Novel Approach for Lifetime-Proportional Luminescence Imaging Using Frame Straddling

Author(s): Soeren Ahmerkamp, Cesar O. Pacherres, Maria Mosshammer, Mathilde Godefroid, Michael Wind-Hansen, Marcel Kuypers, Lars Behrendt, Klaus Koren, Michael Kühl
Published in: ACS Sensors, Issue 9, 2024, ISSN 2379-3694
Publisher: American Chemical Society (ACS)
DOI: 10.1021/ACSSENSORS.4C01828

Luminescence Lifetime Imaging: Key Concepts and Advances in the Image Acquisition, Processing, and Analysis Pipeline

Author(s): Rishi Harkhoe, Gabriele Ferrari, Ruslan I. Dmitriev, Michael Kühl
Published in: ACS Measurement Science Au, 2026, ISSN 2694-250X
Publisher: American Chemical Society (ACS)
DOI: 10.1021/ACSMEASURESCIAU.5C00209

Thou shall not heal: Overcoming the non-healing behaviour of diabetic foot ulcers by engineering the inflammatory microenvironment

Author(s): Michael G. Monaghan, Rajiv Borah, Charlotte Thomsen, Shane Browne,
Published in: Advanced Drug Delivery Reviews, Issue 203, 2023, ISSN 0169-409X
Publisher: Elvesier
DOI: 10.1016/j.addr.2023.115120

<i>In Vivo</i> Lifetime Imaging of the Internal O₂ Dynamics in Corals with near-Infrared-Emitting Sensor Nanoparticles

Author(s): Michael Kühl, Daniel Aagren Nielsen, Sergey M. Borisov
Published in: ACS Sensors, Issue 9, 2024, ISSN 2379-3694
Publisher: American Chemical Society (ACS)
DOI: 10.1021/ACSSENSORS.4C01029

Live Microscopy of Multicellular Spheroids with the Multimodal Near-Infrared Nanoparticles Reveals Differences in Oxygenation Gradients

Author(s): Angela C. Debruyne, Irina A. Okkelman, Nina Heymans, Cláudio Pinheiro, An Hendrix, Max Nobis, Sergey M. Borisov, Ruslan I. Dmitriev
Published in: ACS Nano, Issue 18, 2024, ISSN 1936-0851
Publisher: American Chemical Society (ACS)
DOI: 10.1021/ACSNANO.3C12539

Parallelizing Adaptive Reliability Analysis Through Penalizing the Learning Function

Author(s): Guangchen Wang; Michael Monaghan; Mimi Zhang
Published in: IEEE Transactions on Reliability, 2024
Publisher: IEEE
DOI: 10.1109/TR.2024.3483307

Engineering macrophage responses through 3D scaffold microarchitecture

Author(s): Chiara Martinelli; Srijan Chakraborty; Giovanni Buccioli; Matteo Vicini; Claudio Conci; Giulio Cerullo; Roberto Osellame; Giuseppe Chirico; Emanuela Jacchetti; Manuela Teresa Raimondi
Published in: Materials Today Bio, 2025
Publisher: ELSEVIER
DOI: 10.1016/J.MTBIO.2025.102328

3D photopolymerized microstructured scaffolds influence nuclear deformation, nucleo/cytoskeletal protein organization, and gene regulation in mesenchymal stem cells

Author(s): Francesca Donnaloja; Manuela Teresa Raimondi; Letizia Messa; Bianca Barzaghini; Federica Carnevali; Emanuele Colombo; Davide Mazza; Chiara Martinelli; Lucia Boeri; Federica Rey; Cristina Cereda; Roberto Osellame; Giulio Cerullo; Stephana Carelli; Monica Soncini; Emanuela Jacchetti
Published in: APL Bioengineering, 2023
Publisher: AIP PUBLISHING
DOI: 10.1063/5.0153215

Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding

Author(s): Margarida Barroso, Michael G. Monaghan, Raluca Niesner, Ruslan I. Dmitriev,
Published in: Advanced Drug Delivery Reviews, Issue 201, 2023, ISSN 0169-409X
Publisher: Elvesier
DOI: 10.1016/j.addr.2023.115081

Lack of biochemical signalling in GelMA leads to polarity reversion in intestinal organoids independent from mechanoreciprocity

Author(s): Lenie Vanhove; Thomas Van Gansbeke; Bert Devriendt; Ruben Van der Meeren; Ruslan I. Dmitriev; Irina A. Okkelman
Published in: Journal of Tissue Engineering, 2025
Publisher: SAGE JOURNALS
DOI: 10.1177/20417314251345000

A scanning particle image velocimetry system for spatial mapping of steady flow and <scp>O₂</scp> concentration fields around aquatic organisms

Author(s): Cesar O. Pacherres; Amin A. Huber; Swathi Murthy; Michael Kühl
Published in: Methods in Ecology and Evolution, 2025
Publisher: BRITISH ECOLOGICAL SOCIETY
DOI: 10.1111/2041-210X.70073

Other (2)

Biochemical resolving power of fluorescence lifetime imaging: untangling the roles of the instrument response function and photon-statistics

Author(s): Esposito, Alessandro
Published in: 2023
DOI: 10.5281/zenodo.7796539

ECM Proteins Nidogen‐1 and Decorin Restore Functionality of Human Islets of Langerhans upon Hypoxic Conditions

Author(s): Abiramy Jeyagaran; Max Urbanczyk; Daniel Carvajal‐Berrio; Teresa Baldissera; Philipp D. Kaiser; Laurence Kuhlburger; Stefan Czemmel; Sven Nahnsen; Garry P. Duffy; Sara Y. Brucker; Shannon L. Layland; Katja Schenke‐Layland
Published in: Advanced Healthcare Materials, 2024
DOI: 10.1002/ADHM.202403017