Development of an In-Vivo Brillouin Microscope (with application to Protein Aggregation-based Pathologies)

Project Information

IVBM-4PAP

Grant agreement ID: 101098989

DOI

10.3030/101098989

EC signature date 16 December 2022

Start date 1 March 2023

End date 31 August 2026

Funded under

• The European Innovation Council (EIC)

Total cost

€ 3 868 938,00

EU contribution

€ 3 868 938,00

Coordinated by

FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
Italy

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Data Management Plan (3)

RP2 update of the Data Management Plan
RP1 update of the Data Management Plan
Data Management Plan

Documents, reports (8)

Identification of MLL4 mutations affecting condensates formation
Tau and alpha-synuclein variants
Generation of four endoOpto-MLL4 modules in MSCs
Design of the RF cavity
Tau Biomolecular condensates
Plan for Dissemination and Exploitation including Communication Activities
Project website and logo

Website and project logo information with also social media links

Measurement of nuclear mechanical properties by standard BM

Demonstrators, pilots, prototypes (3)

Fast scanning implemented
Fast scanning simulated
RF cavity ready for the IVBM

Publications

Peer reviewed articles (26)

Computational structural-based GPCR optimization for user-defined ligand: implications for the development of biosensors

Author(s): L. DiRienzo, M. Miotto, E. Milanetti, and G. Ruocco.
Published in: Computational and Structural Biotechnology Journal, ISSN 2001-0370
Publisher: Elsevier
DOI: 10.1016/J.CSBJ.2023.05.004

Journal of Molecular Biology

Author(s): Gian Gaetano Tartaglia, Hanne Hollås, Bjarte Håvik, Anni Vedeler, Annalisa Pastore
Published in: Journal of Molecular Biology, Issue 437, 2025, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/J.JMB.2024.168933

Localised Therapies Using 3D‐Printed Collagen‐Based Micro‐Implant for Ocular Indications

Author(s): Hamid Heidari Kashkooli, Arian Farokh, Sajad Mohammadi, Martina Marcotulli, Silvia Franco, Roberta Angelini, Giancarlo Ruocco, Hanieh Khalili, Gianluca Cidonio
Published in: Macromolecular Materials and Engineering, 2025, ISSN 1438-7492
Publisher: Wiley
DOI: 10.1002/MAME.202400236

Rationalizing the effects of RNA modifications on protein interactions

Author(s): Andrea Vandelli, Laura Broglia, Alexandros Armaos, Riccardo Delli Ponti, Gian Gaetano Tartaglia
Published in: Molecular Therapy - Nucleic Acids, Issue 35, 2024, ISSN 2162-2531
Publisher: Elsevier BV
DOI: 10.1016/J.OMTN.2024.102391

Retinal Fingerprints of ALS in Patients: Ganglion Cell Apoptosis and TDP-43/p62 Misplacement.

Author(s): Pediconi, Natalia; Gigante, Ylenia; Cama, Silvia; Pitea, Martina; Mautone, Lorenza; Ruocco, Giancarlo; Ghirga, Silvia; Di Angelantonio, Silvia
Published in: Frontiers in Aging Neuroscience, 2023, ISSN 1663-4365
Publisher: Frontiers
DOI: 10.3389/fnagi.2023.1110520

Scientific Reports

Author(s): Lorenzo Barolo, Ylenia Gigante, Lorenza Mautone, Silvia Ghirga, Alessandro Soloperto, Alessandra Giorgi, Francesca Ghirga, Martina Pitea, Alessio Incocciati, Francesco Mura, Giancarlo Ruocco, Alberto Boffi, Paola Baiocco, Silvia Di Angelantonio
Published in: Scientific Reports, Issue 14, 2024, ISSN 2045-2322
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41598-024-62188-8

Two receptor binding strategy of SARS-CoV-2 is mediated by both the N-terminal and receptor-binding spike domain

Author(s): M. Monti, E. Milanetti, M. T. Frans, M. Miotto, L. DiRienzo, M. V. Baranov, G. Gosti, A. K. Somavarapu, M. Nagaraj, T. W. Golbeck, E. Rossing, S. J. Moons, T. J. Boltje, G. van der Bogaart,..., G. G. Tartaglia, G. Ruocco, and S. J. Roeters
Published in: Journal of Physical Chemistry B, ISSN 1520-5207
Publisher: American Chemical Society
DOI: 10.1021/ACS.JPCB.3C06258

Exploring the NANOG-TET2 interaction interface. Effects of a selected mutation and hypothesis on the clinical correlation with anemias

Author(s): Claudia Testi, Roberta Piacentini, Alessandro Perrone, Chiara Bartoli, Daniele Leso, Domitilla Pavia, Elisa Pistolesi, Flavio Scipione, Irene Cotronea, Marco Adinolfi Falcone, Marco Ierani, Alberto Boffi, Lorenzo Di Rienzo
Published in: Frontiers in Chemical Biology, Issue 3, 2025, ISSN 2813-530X
Publisher: Frontiers Media SA
DOI: 10.3389/FCHBI.2024.1517163

Nature Communications

Author(s): Johanna Luige, Alexandros Armaos, Gian Gaetano Tartaglia, Ulf Andersson Vang Ørom
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41467-024-46731-9

Molecular dynamics analysis of Superoxide Dismutase 1 mutations suggests decoupling between mechanisms underlying ALS onset and progression

Author(s): M. Kalia, M. Miotto, ..., G. Ruocco, E. Milanetti, R. Dobson, A. Al Chalabi, and A. Iacoangeli.
Published in: Computational and Structural Biotechnology Journal, ISSN 2001-0370
Publisher: ScienceDirect
DOI: 10.1016/J.CSBJ.2023.09.016

Reference-less wavefront shaping in an Hopfield-like rough intensity landscape

Author(s): Marco Leonetti; Luca Leuzzi; Giancarlo Ruocco
Published in: Optic Express, 2023, ISSN 1094-4087
Publisher: Optica Publishing Group
DOI: 10.1364/oe.492055

Design of protein-binding peptides with controlled binding affinity: the case of SARS-CoV-2 receptor binding domain and ACE2-derived peptides

Author(s): G. Parisi, R. Piacentini, A. Incocciati, A. Bonamore, A. Macone, J. Rupert, E. Zacco, M. Miotto, E. Milanetti, G. G. Tartaglia, G. Ruocco, A. Boffi, and L. Di Rienzo.
Published in: Frontiers in Molecular Biosciences, ISSN 2296-889X
Publisher: Frontiers
DOI: 10.3389/FMOLB.2023.1332359

Synchronized motion of interface residues for evaluating protein–<scp>RNA</scp> complex binding affinity: Application to aptamer‐mediated inhibition of <scp>TDP‐43</scp> aggregates

Author(s): Francesco Paolo Panei, Lorenzo Di Rienzo, Elsa Zacco, Alexandros Armaos, Gian Gaetano Tartaglia, Giancarlo Ruocco, Edoardo Milanetti
Published in: Protein Science, Issue 33, 2024, ISSN 0961-8368
Publisher: Wiley
DOI: 10.1002/PRO.5201

RNA sequestration driven by amyloid formation: the alpha synuclein case

Author(s): Jakob Rupert, Michele Monti, Elsa Zacco, Gian Gaetano Tartaglia
Published in: NAR, ISSN 1362-4962
Publisher: Oxford University Press
DOI: 10.1093/NAR/GKAD857

A Model Eye for fluorescent characterization of retinal cultures and tissues

Author(s): G. Ferraro, Y. Gigante, M. Pitea, L. Mautone, G. Ruocco, S. Di Angelantonio, and M. Leonetti.
Published in: Scientific Reports, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/S41598-023-37806-6

Computational evidences of a misfolding event in an aggregation-prone light chain preceding the formation of the non-native pathogenic dimer

Author(s): F. Desantis, M. Miotto, E. Milanetti, G. Ruocco, and L. Di Rienzo.
Published in: Proteins: Structure Function and Bioinformatics, ISSN 1097-0134
Publisher: Wiley
DOI: 10.1002/PROT.26672

Progressive alteration of murine bladder elasticity in actinic cystitis detected by Brillouin microscopy

Author(s): Laura Martinez-Vidal, Claudia Testi, Emanuele Pontecorvo, Filippo Pederzoli, Elisa Alchera, Irene Locatelli, Chiara Venegoni, Antonello Spinelli, Roberta Lucianò, Andrea Salonia, Alessandro Podestà, Giancarlo Ruocco & Massimo Alfano
Published in: Scientific Reports, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/S41598-023-51006-2

Differences in the organization of interface residues tunes the stability of the SARS-CoV-2 spike-ACE2 complex

Author(s): Mattia, Miotto; Lorenzo, Di Rienzo; Greta, Grassmann; Fausta, Desantis; Gianluca, Cidonio; Giorgio, Gosti; Marco, Leonetti; Giancarlo, Ruocco; Edoardo, Milanetti
Published in: Frontiers in Molecular Biosciences, 2023, ISSN 2296-889X
Publisher: Frontiers
DOI: 10.3389/fmolb.2023.1205919

Osmolyte-induced protein stability changes explained by graph theory

Author(s): Mattia Miotto, Nina Warner, Giancarlo Ruocco, Gian Gaetano Tartaglia, Oren A. Scherman, Edoardo Milanetti
Published in: Computational and Structural Biotechnology Journal, Issue 23, 2025, ISSN 2001-0370
Publisher: Elsevier BV
DOI: 10.1016/J.CSBJ.2024.10.014

A size-dependent division strategy accounts for leukemia cell size heterogeneity

Author(s): Mattia Miotto, Simone Scalise, Marco Leonetti, Giancarlo Ruocco, Giovanna Peruzzi, Giorgio Gosti
Published in: Communications Physics, 2024, ISSN 2399-3650
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S42005-024-01743-1

Computational Approaches to Predict Protein–Protein Interactions in Crowded Cellular Environments

Author(s): Greta Grassmann, Mattia Miotto, Fausta Desantis, Lorenzo Di Rienzo, Gian Gaetano Tartaglia, Annalisa Pastore, Giancarlo Ruocco, Michele Monti, Edoardo Milanetti
Published in: Chemical Reviews, Issue 124, 2024, ISSN 0009-2665
Publisher: American Chemical Society (ACS)
DOI: 10.1021/ACS.CHEMREV.3C00550

Journal of Physical Chemistry Letters

Author(s): Mattia Miotto, Edoardo Milanetti, Riccardo Mincigrucci, Claudio Masciovecchio, Giancarlo Ruocco
Published in: The Journal of Physical Chemistry Letters, Issue 15, 2024, ISSN 1948-7185
Publisher: American Chemical Society (ACS)
DOI: 10.1021/ACS.JPCLETT.3C03632

Prediction of protein-RNA interactions from single-cell transcriptomic data

Author(s): Jonathan Fiorentino, Alexandros Armaos, Alessio Colantoni, Gian Gaetano Tartaglia
Published in: Nucleic Acids Research, ISSN 1362-4962
Publisher: Oxford University Press
DOI: 10.1093/NAR/GKAE076

Electrostatic complementarity at the interface drives transient protein-protein interactions

Author(s): G. Grassmann, L. Di Rienzo, G. Gosti, M. Leonetti, G. Ruocco, M. Miotto, and E. Milanetti.
Published in: Scientific Reports, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/S41598-023-37130-Z

Advantages of integrating Brillouin microscopy in multimodal mechanical mapping of cells and tissues

Author(s): Chenchen Handler, Claudia Testi, Giuliano Scarcelli
Published in: Current Opinion in Cell Biology, Issue 88, 2024, ISSN 0955-0674
Publisher: Elsevier BV
DOI: 10.1016/J.CEB.2024.102341

Stochastically structured illumination microscopy scan less super resolution imaging

Author(s): Denzel Fusco, Emmanouil Xypakis, Ylenia Gigante, Lorenza Mautone, Silvia Di Angelantonio, Giorgia Ponsi, Giancarlo Ruocco, Marco Leonetti
Published in: npj Imaging, Issue 2, 2024, ISSN 2948-197X
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S44303-024-00047-X

Other (2)

Collective behavior and self-organization in neural rosette morphogenesis

Author(s): M. Miotto, M. Rosito, M. Paoluzi, V. de Turris, V. Folli, M. Leonetti, G. Ruocco, A. Rosa, and G. Gosti.
Published in: Frontiers in Cell and Developmental Biology, ISSN 2296-634X
Publisher: Frontiers
DOI: 10.3389/FCELL.2023.1134091

May the force be with you: Nuclear condensates function beyond transcription control: Potential nongenetic functions of nuclear condensates in physiological and pathological conditions

Author(s): Negri Maria Luce, D'Annunzio Sarah, Vitali Giulia, Zippo Alessio
Published in: BioEssays, ISSN 1521-1878
Publisher: WILEY Online Library
DOI: 10.1002/BIES.202300075