Development of an In-Vivo Brillouin Microscope (with application to Protein Aggregation-based Pathologies)

Información del proyecto

IVBM-4PAP

Identificador del acuerdo de subvención: 101098989

DOI

10.3030/101098989

Fecha de la firma de la CE 16 Diciembre 2022

Fecha de inicio 1 Marzo 2023

Fecha de finalización 31 Agosto 2026

Financiado con arreglo a

• The European Innovation Council (EIC)

Coste total

€ 3 868 938,00

Aportación de la UE

€ 3 868 938,00

Coordinado por

FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
Italy

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Data Management Plan (3)

RP2 update of the Data Management Plan
RP1 update of the Data Management Plan
Data Management Plan

Documents, reports (8)

Identification of MLL4 mutations affecting condensates formation
Tau and alpha-synuclein variants
Generation of four endoOpto-MLL4 modules in MSCs
Design of the RF cavity
Tau Biomolecular condensates
Plan for Dissemination and Exploitation including Communication Activities
Project website and logo

Website and project logo information with also social media links

Measurement of nuclear mechanical properties by standard BM

Demonstrators, pilots, prototypes (3)

Fast scanning implemented
Fast scanning simulated
RF cavity ready for the IVBM

Publicaciones

Peer reviewed articles (26)

Computational structural-based GPCR optimization for user-defined ligand: implications for the development of biosensors

Autores: L. DiRienzo, M. Miotto, E. Milanetti, and G. Ruocco.
Publicado en: Computational and Structural Biotechnology Journal, ISSN 2001-0370
Editor: Elsevier
DOI: 10.1016/J.CSBJ.2023.05.004

Journal of Molecular Biology

Autores: Gian Gaetano Tartaglia, Hanne Hollås, Bjarte Håvik, Anni Vedeler, Annalisa Pastore
Publicado en: Journal of Molecular Biology, Edición 437, 2025, ISSN 0022-2836
Editor: Academic Press
DOI: 10.1016/J.JMB.2024.168933

Localised Therapies Using 3D‐Printed Collagen‐Based Micro‐Implant for Ocular Indications

Autores: Hamid Heidari Kashkooli, Arian Farokh, Sajad Mohammadi, Martina Marcotulli, Silvia Franco, Roberta Angelini, Giancarlo Ruocco, Hanieh Khalili, Gianluca Cidonio
Publicado en: Macromolecular Materials and Engineering, 2025, ISSN 1438-7492
Editor: Wiley
DOI: 10.1002/MAME.202400236

Rationalizing the effects of RNA modifications on protein interactions

Autores: Andrea Vandelli, Laura Broglia, Alexandros Armaos, Riccardo Delli Ponti, Gian Gaetano Tartaglia
Publicado en: Molecular Therapy - Nucleic Acids, Edición 35, 2024, ISSN 2162-2531
Editor: Elsevier BV
DOI: 10.1016/J.OMTN.2024.102391

Retinal Fingerprints of ALS in Patients: Ganglion Cell Apoptosis and TDP-43/p62 Misplacement.

Autores: Pediconi, Natalia; Gigante, Ylenia; Cama, Silvia; Pitea, Martina; Mautone, Lorenza; Ruocco, Giancarlo; Ghirga, Silvia; Di Angelantonio, Silvia
Publicado en: Frontiers in Aging Neuroscience, 2023, ISSN 1663-4365
Editor: Frontiers
DOI: 10.3389/fnagi.2023.1110520

Scientific Reports

Autores: Lorenzo Barolo, Ylenia Gigante, Lorenza Mautone, Silvia Ghirga, Alessandro Soloperto, Alessandra Giorgi, Francesca Ghirga, Martina Pitea, Alessio Incocciati, Francesco Mura, Giancarlo Ruocco, Alberto Boffi, Paola Baiocco, Silvia Di Angelantonio
Publicado en: Scientific Reports, Edición 14, 2024, ISSN 2045-2322
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41598-024-62188-8

Two receptor binding strategy of SARS-CoV-2 is mediated by both the N-terminal and receptor-binding spike domain

Autores: M. Monti, E. Milanetti, M. T. Frans, M. Miotto, L. DiRienzo, M. V. Baranov, G. Gosti, A. K. Somavarapu, M. Nagaraj, T. W. Golbeck, E. Rossing, S. J. Moons, T. J. Boltje, G. van der Bogaart,..., G. G. Tartaglia, G. Ruocco, and S. J. Roeters
Publicado en: Journal of Physical Chemistry B, ISSN 1520-5207
Editor: American Chemical Society
DOI: 10.1021/ACS.JPCB.3C06258

Exploring the NANOG-TET2 interaction interface. Effects of a selected mutation and hypothesis on the clinical correlation with anemias

Autores: Claudia Testi, Roberta Piacentini, Alessandro Perrone, Chiara Bartoli, Daniele Leso, Domitilla Pavia, Elisa Pistolesi, Flavio Scipione, Irene Cotronea, Marco Adinolfi Falcone, Marco Ierani, Alberto Boffi, Lorenzo Di Rienzo
Publicado en: Frontiers in Chemical Biology, Edición 3, 2025, ISSN 2813-530X
Editor: Frontiers Media SA
DOI: 10.3389/FCHBI.2024.1517163

Nature Communications

Autores: Johanna Luige, Alexandros Armaos, Gian Gaetano Tartaglia, Ulf Andersson Vang Ørom
Publicado en: Nature Communications, Edición 15, 2024, ISSN 2041-1723
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41467-024-46731-9

Molecular dynamics analysis of Superoxide Dismutase 1 mutations suggests decoupling between mechanisms underlying ALS onset and progression

Autores: M. Kalia, M. Miotto, ..., G. Ruocco, E. Milanetti, R. Dobson, A. Al Chalabi, and A. Iacoangeli.
Publicado en: Computational and Structural Biotechnology Journal, ISSN 2001-0370
Editor: ScienceDirect
DOI: 10.1016/J.CSBJ.2023.09.016

Reference-less wavefront shaping in an Hopfield-like rough intensity landscape

Autores: Marco Leonetti; Luca Leuzzi; Giancarlo Ruocco
Publicado en: Optic Express, 2023, ISSN 1094-4087
Editor: Optica Publishing Group
DOI: 10.1364/oe.492055

Design of protein-binding peptides with controlled binding affinity: the case of SARS-CoV-2 receptor binding domain and ACE2-derived peptides

Autores: G. Parisi, R. Piacentini, A. Incocciati, A. Bonamore, A. Macone, J. Rupert, E. Zacco, M. Miotto, E. Milanetti, G. G. Tartaglia, G. Ruocco, A. Boffi, and L. Di Rienzo.
Publicado en: Frontiers in Molecular Biosciences, ISSN 2296-889X
Editor: Frontiers
DOI: 10.3389/FMOLB.2023.1332359

Synchronized motion of interface residues for evaluating protein–<scp>RNA</scp> complex binding affinity: Application to aptamer‐mediated inhibition of <scp>TDP‐43</scp> aggregates

Autores: Francesco Paolo Panei, Lorenzo Di Rienzo, Elsa Zacco, Alexandros Armaos, Gian Gaetano Tartaglia, Giancarlo Ruocco, Edoardo Milanetti
Publicado en: Protein Science, Edición 33, 2024, ISSN 0961-8368
Editor: Wiley
DOI: 10.1002/PRO.5201

RNA sequestration driven by amyloid formation: the alpha synuclein case

Autores: Jakob Rupert, Michele Monti, Elsa Zacco, Gian Gaetano Tartaglia
Publicado en: NAR, ISSN 1362-4962
Editor: Oxford University Press
DOI: 10.1093/NAR/GKAD857

A Model Eye for fluorescent characterization of retinal cultures and tissues

Autores: G. Ferraro, Y. Gigante, M. Pitea, L. Mautone, G. Ruocco, S. Di Angelantonio, and M. Leonetti.
Publicado en: Scientific Reports, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/S41598-023-37806-6

Computational evidences of a misfolding event in an aggregation-prone light chain preceding the formation of the non-native pathogenic dimer

Autores: F. Desantis, M. Miotto, E. Milanetti, G. Ruocco, and L. Di Rienzo.
Publicado en: Proteins: Structure Function and Bioinformatics, ISSN 1097-0134
Editor: Wiley
DOI: 10.1002/PROT.26672

Progressive alteration of murine bladder elasticity in actinic cystitis detected by Brillouin microscopy

Autores: Laura Martinez-Vidal, Claudia Testi, Emanuele Pontecorvo, Filippo Pederzoli, Elisa Alchera, Irene Locatelli, Chiara Venegoni, Antonello Spinelli, Roberta Lucianò, Andrea Salonia, Alessandro Podestà, Giancarlo Ruocco & Massimo Alfano
Publicado en: Scientific Reports, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/S41598-023-51006-2

Differences in the organization of interface residues tunes the stability of the SARS-CoV-2 spike-ACE2 complex

Autores: Mattia, Miotto; Lorenzo, Di Rienzo; Greta, Grassmann; Fausta, Desantis; Gianluca, Cidonio; Giorgio, Gosti; Marco, Leonetti; Giancarlo, Ruocco; Edoardo, Milanetti
Publicado en: Frontiers in Molecular Biosciences, 2023, ISSN 2296-889X
Editor: Frontiers
DOI: 10.3389/fmolb.2023.1205919

Osmolyte-induced protein stability changes explained by graph theory

Autores: Mattia Miotto, Nina Warner, Giancarlo Ruocco, Gian Gaetano Tartaglia, Oren A. Scherman, Edoardo Milanetti
Publicado en: Computational and Structural Biotechnology Journal, Edición 23, 2025, ISSN 2001-0370
Editor: Elsevier BV
DOI: 10.1016/J.CSBJ.2024.10.014

A size-dependent division strategy accounts for leukemia cell size heterogeneity

Autores: Mattia Miotto, Simone Scalise, Marco Leonetti, Giancarlo Ruocco, Giovanna Peruzzi, Giorgio Gosti
Publicado en: Communications Physics, 2024, ISSN 2399-3650
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S42005-024-01743-1

Computational Approaches to Predict Protein–Protein Interactions in Crowded Cellular Environments

Autores: Greta Grassmann, Mattia Miotto, Fausta Desantis, Lorenzo Di Rienzo, Gian Gaetano Tartaglia, Annalisa Pastore, Giancarlo Ruocco, Michele Monti, Edoardo Milanetti
Publicado en: Chemical Reviews, Edición 124, 2024, ISSN 0009-2665
Editor: American Chemical Society (ACS)
DOI: 10.1021/ACS.CHEMREV.3C00550

Journal of Physical Chemistry Letters

Autores: Mattia Miotto, Edoardo Milanetti, Riccardo Mincigrucci, Claudio Masciovecchio, Giancarlo Ruocco
Publicado en: The Journal of Physical Chemistry Letters, Edición 15, 2024, ISSN 1948-7185
Editor: American Chemical Society (ACS)
DOI: 10.1021/ACS.JPCLETT.3C03632

Prediction of protein-RNA interactions from single-cell transcriptomic data

Autores: Jonathan Fiorentino, Alexandros Armaos, Alessio Colantoni, Gian Gaetano Tartaglia
Publicado en: Nucleic Acids Research, ISSN 1362-4962
Editor: Oxford University Press
DOI: 10.1093/NAR/GKAE076

Electrostatic complementarity at the interface drives transient protein-protein interactions

Autores: G. Grassmann, L. Di Rienzo, G. Gosti, M. Leonetti, G. Ruocco, M. Miotto, and E. Milanetti.
Publicado en: Scientific Reports, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/S41598-023-37130-Z

Advantages of integrating Brillouin microscopy in multimodal mechanical mapping of cells and tissues

Autores: Chenchen Handler, Claudia Testi, Giuliano Scarcelli
Publicado en: Current Opinion in Cell Biology, Edición 88, 2024, ISSN 0955-0674
Editor: Elsevier BV
DOI: 10.1016/J.CEB.2024.102341

Stochastically structured illumination microscopy scan less super resolution imaging

Autores: Denzel Fusco, Emmanouil Xypakis, Ylenia Gigante, Lorenza Mautone, Silvia Di Angelantonio, Giorgia Ponsi, Giancarlo Ruocco, Marco Leonetti
Publicado en: npj Imaging, Edición 2, 2024, ISSN 2948-197X
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S44303-024-00047-X

Other (2)

Collective behavior and self-organization in neural rosette morphogenesis

Autores: M. Miotto, M. Rosito, M. Paoluzi, V. de Turris, V. Folli, M. Leonetti, G. Ruocco, A. Rosa, and G. Gosti.
Publicado en: Frontiers in Cell and Developmental Biology, ISSN 2296-634X
Editor: Frontiers
DOI: 10.3389/FCELL.2023.1134091

May the force be with you: Nuclear condensates function beyond transcription control: Potential nongenetic functions of nuclear condensates in physiological and pathological conditions

Autores: Negri Maria Luce, D'Annunzio Sarah, Vitali Giulia, Zippo Alessio
Publicado en: BioEssays, ISSN 1521-1878
Editor: WILEY Online Library
DOI: 10.1002/BIES.202300075