Development of an In-Vivo Brillouin Microscope (with application to Protein Aggregation-based Pathologies)

Informations projet

IVBM-4PAP

N° de convention de subvention: 101098989

DOI

10.3030/101098989

Date de signature de la CE 16 Decembre 2022

Date de début 1 Mars 2023

Date de fin 31 Août 2026

Financé au titre de

• The European Innovation Council (EIC)

Coût total

€ 3 868 938,00

Contribution de l’UE

€ 3 868 938,00

Coordonné par

FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
Italy

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Data Management Plan (3)

RP2 update of the Data Management Plan
RP1 update of the Data Management Plan
Data Management Plan

Documents, reports (8)

Identification of MLL4 mutations affecting condensates formation
Tau and alpha-synuclein variants
Generation of four endoOpto-MLL4 modules in MSCs
Design of the RF cavity
Tau Biomolecular condensates
Plan for Dissemination and Exploitation including Communication Activities
Project website and logo

Website and project logo information with also social media links

Measurement of nuclear mechanical properties by standard BM

Demonstrators, pilots, prototypes (3)

Fast scanning implemented
Fast scanning simulated
RF cavity ready for the IVBM

Publications

Peer reviewed articles (26)

Computational structural-based GPCR optimization for user-defined ligand: implications for the development of biosensors

Auteurs: L. DiRienzo, M. Miotto, E. Milanetti, and G. Ruocco.
Publié dans: Computational and Structural Biotechnology Journal, ISSN 2001-0370
Éditeur: Elsevier
DOI: 10.1016/J.CSBJ.2023.05.004

Journal of Molecular Biology

Auteurs: Gian Gaetano Tartaglia, Hanne Hollås, Bjarte Håvik, Anni Vedeler, Annalisa Pastore
Publié dans: Journal of Molecular Biology, Numéro 437, 2025, ISSN 0022-2836
Éditeur: Academic Press
DOI: 10.1016/J.JMB.2024.168933

Localised Therapies Using 3D‐Printed Collagen‐Based Micro‐Implant for Ocular Indications

Auteurs: Hamid Heidari Kashkooli, Arian Farokh, Sajad Mohammadi, Martina Marcotulli, Silvia Franco, Roberta Angelini, Giancarlo Ruocco, Hanieh Khalili, Gianluca Cidonio
Publié dans: Macromolecular Materials and Engineering, 2025, ISSN 1438-7492
Éditeur: Wiley
DOI: 10.1002/MAME.202400236

Rationalizing the effects of RNA modifications on protein interactions

Auteurs: Andrea Vandelli, Laura Broglia, Alexandros Armaos, Riccardo Delli Ponti, Gian Gaetano Tartaglia
Publié dans: Molecular Therapy - Nucleic Acids, Numéro 35, 2024, ISSN 2162-2531
Éditeur: Elsevier BV
DOI: 10.1016/J.OMTN.2024.102391

Retinal Fingerprints of ALS in Patients: Ganglion Cell Apoptosis and TDP-43/p62 Misplacement.

Auteurs: Pediconi, Natalia; Gigante, Ylenia; Cama, Silvia; Pitea, Martina; Mautone, Lorenza; Ruocco, Giancarlo; Ghirga, Silvia; Di Angelantonio, Silvia
Publié dans: Frontiers in Aging Neuroscience, 2023, ISSN 1663-4365
Éditeur: Frontiers
DOI: 10.3389/fnagi.2023.1110520

Scientific Reports

Auteurs: Lorenzo Barolo, Ylenia Gigante, Lorenza Mautone, Silvia Ghirga, Alessandro Soloperto, Alessandra Giorgi, Francesca Ghirga, Martina Pitea, Alessio Incocciati, Francesco Mura, Giancarlo Ruocco, Alberto Boffi, Paola Baiocco, Silvia Di Angelantonio
Publié dans: Scientific Reports, Numéro 14, 2024, ISSN 2045-2322
Éditeur: Springer Science and Business Media LLC
DOI: 10.1038/S41598-024-62188-8

Two receptor binding strategy of SARS-CoV-2 is mediated by both the N-terminal and receptor-binding spike domain

Auteurs: M. Monti, E. Milanetti, M. T. Frans, M. Miotto, L. DiRienzo, M. V. Baranov, G. Gosti, A. K. Somavarapu, M. Nagaraj, T. W. Golbeck, E. Rossing, S. J. Moons, T. J. Boltje, G. van der Bogaart,..., G. G. Tartaglia, G. Ruocco, and S. J. Roeters
Publié dans: Journal of Physical Chemistry B, ISSN 1520-5207
Éditeur: American Chemical Society
DOI: 10.1021/ACS.JPCB.3C06258

Exploring the NANOG-TET2 interaction interface. Effects of a selected mutation and hypothesis on the clinical correlation with anemias

Auteurs: Claudia Testi, Roberta Piacentini, Alessandro Perrone, Chiara Bartoli, Daniele Leso, Domitilla Pavia, Elisa Pistolesi, Flavio Scipione, Irene Cotronea, Marco Adinolfi Falcone, Marco Ierani, Alberto Boffi, Lorenzo Di Rienzo
Publié dans: Frontiers in Chemical Biology, Numéro 3, 2025, ISSN 2813-530X
Éditeur: Frontiers Media SA
DOI: 10.3389/FCHBI.2024.1517163

Nature Communications

Auteurs: Johanna Luige, Alexandros Armaos, Gian Gaetano Tartaglia, Ulf Andersson Vang Ørom
Publié dans: Nature Communications, Numéro 15, 2024, ISSN 2041-1723
Éditeur: Springer Science and Business Media LLC
DOI: 10.1038/S41467-024-46731-9

Molecular dynamics analysis of Superoxide Dismutase 1 mutations suggests decoupling between mechanisms underlying ALS onset and progression

Auteurs: M. Kalia, M. Miotto, ..., G. Ruocco, E. Milanetti, R. Dobson, A. Al Chalabi, and A. Iacoangeli.
Publié dans: Computational and Structural Biotechnology Journal, ISSN 2001-0370
Éditeur: ScienceDirect
DOI: 10.1016/J.CSBJ.2023.09.016

Reference-less wavefront shaping in an Hopfield-like rough intensity landscape

Auteurs: Marco Leonetti; Luca Leuzzi; Giancarlo Ruocco
Publié dans: Optic Express, 2023, ISSN 1094-4087
Éditeur: Optica Publishing Group
DOI: 10.1364/oe.492055

Design of protein-binding peptides with controlled binding affinity: the case of SARS-CoV-2 receptor binding domain and ACE2-derived peptides

Auteurs: G. Parisi, R. Piacentini, A. Incocciati, A. Bonamore, A. Macone, J. Rupert, E. Zacco, M. Miotto, E. Milanetti, G. G. Tartaglia, G. Ruocco, A. Boffi, and L. Di Rienzo.
Publié dans: Frontiers in Molecular Biosciences, ISSN 2296-889X
Éditeur: Frontiers
DOI: 10.3389/FMOLB.2023.1332359

Synchronized motion of interface residues for evaluating protein–<scp>RNA</scp> complex binding affinity: Application to aptamer‐mediated inhibition of <scp>TDP‐43</scp> aggregates

Auteurs: Francesco Paolo Panei, Lorenzo Di Rienzo, Elsa Zacco, Alexandros Armaos, Gian Gaetano Tartaglia, Giancarlo Ruocco, Edoardo Milanetti
Publié dans: Protein Science, Numéro 33, 2024, ISSN 0961-8368
Éditeur: Wiley
DOI: 10.1002/PRO.5201

RNA sequestration driven by amyloid formation: the alpha synuclein case

Auteurs: Jakob Rupert, Michele Monti, Elsa Zacco, Gian Gaetano Tartaglia
Publié dans: NAR, ISSN 1362-4962
Éditeur: Oxford University Press
DOI: 10.1093/NAR/GKAD857

A Model Eye for fluorescent characterization of retinal cultures and tissues

Auteurs: G. Ferraro, Y. Gigante, M. Pitea, L. Mautone, G. Ruocco, S. Di Angelantonio, and M. Leonetti.
Publié dans: Scientific Reports, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/S41598-023-37806-6

Computational evidences of a misfolding event in an aggregation-prone light chain preceding the formation of the non-native pathogenic dimer

Auteurs: F. Desantis, M. Miotto, E. Milanetti, G. Ruocco, and L. Di Rienzo.
Publié dans: Proteins: Structure Function and Bioinformatics, ISSN 1097-0134
Éditeur: Wiley
DOI: 10.1002/PROT.26672

Progressive alteration of murine bladder elasticity in actinic cystitis detected by Brillouin microscopy

Auteurs: Laura Martinez-Vidal, Claudia Testi, Emanuele Pontecorvo, Filippo Pederzoli, Elisa Alchera, Irene Locatelli, Chiara Venegoni, Antonello Spinelli, Roberta Lucianò, Andrea Salonia, Alessandro Podestà, Giancarlo Ruocco & Massimo Alfano
Publié dans: Scientific Reports, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/S41598-023-51006-2

Differences in the organization of interface residues tunes the stability of the SARS-CoV-2 spike-ACE2 complex

Auteurs: Mattia, Miotto; Lorenzo, Di Rienzo; Greta, Grassmann; Fausta, Desantis; Gianluca, Cidonio; Giorgio, Gosti; Marco, Leonetti; Giancarlo, Ruocco; Edoardo, Milanetti
Publié dans: Frontiers in Molecular Biosciences, 2023, ISSN 2296-889X
Éditeur: Frontiers
DOI: 10.3389/fmolb.2023.1205919

Osmolyte-induced protein stability changes explained by graph theory

Auteurs: Mattia Miotto, Nina Warner, Giancarlo Ruocco, Gian Gaetano Tartaglia, Oren A. Scherman, Edoardo Milanetti
Publié dans: Computational and Structural Biotechnology Journal, Numéro 23, 2025, ISSN 2001-0370
Éditeur: Elsevier BV
DOI: 10.1016/J.CSBJ.2024.10.014

A size-dependent division strategy accounts for leukemia cell size heterogeneity

Auteurs: Mattia Miotto, Simone Scalise, Marco Leonetti, Giancarlo Ruocco, Giovanna Peruzzi, Giorgio Gosti
Publié dans: Communications Physics, 2024, ISSN 2399-3650
Éditeur: Springer Science and Business Media LLC
DOI: 10.1038/S42005-024-01743-1

Computational Approaches to Predict Protein–Protein Interactions in Crowded Cellular Environments

Auteurs: Greta Grassmann, Mattia Miotto, Fausta Desantis, Lorenzo Di Rienzo, Gian Gaetano Tartaglia, Annalisa Pastore, Giancarlo Ruocco, Michele Monti, Edoardo Milanetti
Publié dans: Chemical Reviews, Numéro 124, 2024, ISSN 0009-2665
Éditeur: American Chemical Society (ACS)
DOI: 10.1021/ACS.CHEMREV.3C00550

Journal of Physical Chemistry Letters

Auteurs: Mattia Miotto, Edoardo Milanetti, Riccardo Mincigrucci, Claudio Masciovecchio, Giancarlo Ruocco
Publié dans: The Journal of Physical Chemistry Letters, Numéro 15, 2024, ISSN 1948-7185
Éditeur: American Chemical Society (ACS)
DOI: 10.1021/ACS.JPCLETT.3C03632

Prediction of protein-RNA interactions from single-cell transcriptomic data

Auteurs: Jonathan Fiorentino, Alexandros Armaos, Alessio Colantoni, Gian Gaetano Tartaglia
Publié dans: Nucleic Acids Research, ISSN 1362-4962
Éditeur: Oxford University Press
DOI: 10.1093/NAR/GKAE076

Electrostatic complementarity at the interface drives transient protein-protein interactions

Auteurs: G. Grassmann, L. Di Rienzo, G. Gosti, M. Leonetti, G. Ruocco, M. Miotto, and E. Milanetti.
Publié dans: Scientific Reports, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/S41598-023-37130-Z

Advantages of integrating Brillouin microscopy in multimodal mechanical mapping of cells and tissues

Auteurs: Chenchen Handler, Claudia Testi, Giuliano Scarcelli
Publié dans: Current Opinion in Cell Biology, Numéro 88, 2024, ISSN 0955-0674
Éditeur: Elsevier BV
DOI: 10.1016/J.CEB.2024.102341

Stochastically structured illumination microscopy scan less super resolution imaging

Auteurs: Denzel Fusco, Emmanouil Xypakis, Ylenia Gigante, Lorenza Mautone, Silvia Di Angelantonio, Giorgia Ponsi, Giancarlo Ruocco, Marco Leonetti
Publié dans: npj Imaging, Numéro 2, 2024, ISSN 2948-197X
Éditeur: Springer Science and Business Media LLC
DOI: 10.1038/S44303-024-00047-X

Other (2)

Collective behavior and self-organization in neural rosette morphogenesis

Auteurs: M. Miotto, M. Rosito, M. Paoluzi, V. de Turris, V. Folli, M. Leonetti, G. Ruocco, A. Rosa, and G. Gosti.
Publié dans: Frontiers in Cell and Developmental Biology, ISSN 2296-634X
Éditeur: Frontiers
DOI: 10.3389/FCELL.2023.1134091

May the force be with you: Nuclear condensates function beyond transcription control: Potential nongenetic functions of nuclear condensates in physiological and pathological conditions

Auteurs: Negri Maria Luce, D'Annunzio Sarah, Vitali Giulia, Zippo Alessio
Publié dans: BioEssays, ISSN 1521-1878
Éditeur: WILEY Online Library
DOI: 10.1002/BIES.202300075