Periodic Reporting for period 1 - LUCRA (SustainabLe sUCcinic acid production using an integRAted electrochemical bioreactor and renewable feedstock)
Periodo di rendicontazione: 2023-07-01 al 2024-12-31
LUCRA will exploit the EU’s underutilized and abundant, Municipal Solid Waste and Wood waste as feedstocks for the large scale production of platform building block biobased chemicals at high yield with significant industrial end-user interest using a circular bioeconomy biorefinery approach. It will provide optimized and scaleable ‘breakthrough first of a kind’ innovative technology to create a step change from pilot to large scale integrated fermentation with novel electrochemical extraction of succinic acid (SA) at TRL7 that will facilitate its industrial implementation, business models and market feasibility. This will be achieved by the circular utilization of the mentioned renewable resources as feedstocks which will reduce to a large extent the dependence on fossil resources and achieves at the same time a sustainable SA production route. To fit the needs of the industrial level, LUCRA will optimize the hydrolysis process of the different feedstocks to obtain efficient cellulose and hemicellulose conversion. Enzymatic cocktails will be demonstrated aiming to maximize hydrolysis of fermentable carbohydrate content with low inhibitors production. The crude sugar-rich hydrolysates will be used as fermentation feedstock in an electrochemical membrane bioreactor for efficient extraction and production of SA. In situ extraction of SA will be achieved through an anion exchange membrane resulting in simultaneous acidification, concentration, lower NaOH consumption for pH regulation and less unit operations for SA purification. To demonstrated the produced SA that will be able to compete directly their fossil-based counterparts from petrochemical processes, different polyurethane dispersions and resins will be produced at semi-industrial scale. The process implemented in LUCRA targeting to reduce the cost of bio-SA and will demonstrate an 50% GHG reduction against conventional SA.
After months of dedicated collaboration, our efforts are starting to pay off—from optimizing organic waste pre-treatment and improving fermentation performance to preparing the biorefinery for demonstration and laying the groundwork for sustainability assessments.
Turning Waste into Value: Optimizing Biomass for Sustainable Biofactories: In the drive to transform organic waste into valuable bioproducts LUCRA has made significant efforts. The team analyzed the availability and composition of organic municipal waste (OFMSW) and sawdust, uncovering seasonal trends and confirming sawdust’s year-round consistency. Through detailed feedstock characterization, key components like starch, lipids, and free sugars were identified, highlighting variations crucial for downstream processing.
Advanced pre-treatment and enzymatic hydrolysis techniques were tested and optimized, achieving high sugar and nutrient yields. By fine-tuning factors such as enzyme dosing, pH levels, and pre-treatment methods—including pressurized hot water and green solvents—the project demonstrated efficient conversion of waste into valuable building blocks for bio-based production.
Advancing Sustainable Succinic Acid Production in LUCRA: Over the past months, the LUCRA project made significant progress in optimizing bio-based succinic acid production using E. coli, achieving yields and productivities that exceed previous benchmarks. Fermentation was successfully tested with various carbon sources, and ideal conditions were identified for improved yields and reduced by-products. Electrochemical extraction systems were further developed. In the next phase, the focus will shift to refining process models, integrating lab-scale systems, and preparing for scale-up to pilot production.
Biorefinery Demonstration: A first version of the piping and instrumentation diagrams has been developed and a electrodialysis stack—suitable for both 150 L and 1500 L fermentations—is ready to be ordered. Pretreatment of municipal solid waste yielded a sugar-rich stream, setting the stage for fermentation trials. In the coming months, engineering and procurement will continue, with real-time process monitoring and biorefinery commissioning planned throughout 2025.
Laying the Foundation for LUCRA’s Sustainability and Economic Assessment: LUCRA is setting up the framework for life cycle (LCA) and techno-economic (TEA) assessments to evaluate the environmental and economic performance of LUCRA’s value chains. Based on process designs from the partners, the team is identifying key sustainability indicators, relevant feedstocks, and final applications. This groundwork enables targeted data collection and delivers valuable feedback to optimize technical processes. The selected indicators will also support the evaluation of circularity and end-of-life scenarios for LUCRA’s bio-based products.
Turning Waste into Value: Optimizing Biomass for Sustainable Biofactories: In the drive to transform organic waste into valuable bioproducts LUCRA has made significant efforts. The team analyzed the availability and composition of organic municipal waste (OFMSW) and sawdust, uncovering seasonal trends and confirming sawdust’s year-round consistency. Through detailed feedstock characterization, key components like starch, lipids, and free sugars were identified, highlighting variations crucial for downstream processing.
Advanced pre-treatment and enzymatic hydrolysis techniques were tested and optimized, achieving high sugar and nutrient yields. By fine-tuning factors such as enzyme dosing, pH levels, and pre-treatment methods—including pressurized hot water and green solvents—the project demonstrated efficient conversion of waste into valuable building blocks for bio-based production.
Advancing Sustainable Succinic Acid Production in LUCRA: Over the past months, the LUCRA project made significant progress in optimizing bio-based succinic acid production using E. coli, achieving yields and productivities that exceed previous benchmarks. Fermentation was successfully tested with various carbon sources, and ideal conditions were identified for improved yields and reduced by-products. Electrochemical extraction systems were further developed. In the next phase, the focus will shift to refining process models, integrating lab-scale systems, and preparing for scale-up to pilot production.
Biorefinery Demonstration: A first version of the piping and instrumentation diagrams has been developed and a electrodialysis stack—suitable for both 150 L and 1500 L fermentations—is ready to be ordered. Pretreatment of municipal solid waste yielded a sugar-rich stream, setting the stage for fermentation trials. In the coming months, engineering and procurement will continue, with real-time process monitoring and biorefinery commissioning planned throughout 2025.
Laying the Foundation for LUCRA’s Sustainability and Economic Assessment: LUCRA is setting up the framework for life cycle (LCA) and techno-economic (TEA) assessments to evaluate the environmental and economic performance of LUCRA’s value chains. Based on process designs from the partners, the team is identifying key sustainability indicators, relevant feedstocks, and final applications. This groundwork enables targeted data collection and delivers valuable feedback to optimize technical processes. The selected indicators will also support the evaluation of circularity and end-of-life scenarios for LUCRA’s bio-based products.
Laying the Foundation for LUCRA’s Sustainability and Economic Assessment: LUCRA is setting up the framework for life cycle (LCA) and techno-economic (TEA) assessments to evaluate the environmental and economic performance of LUCRA’s value chains. Based on process designs from the partners, the team is identifying key sustainability indicators, relevant feedstocks, and final applications. This groundwork enables targeted data collection and delivers valuable feedback to optimize technical processes. The selected indicators will also support the evaluation of circularity and end-of-life scenarios for LUCRA’s bio-based products.