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Multidimensional in vivo metabolic flux analyses: Resolving immune cells based on in vivo metabolic phenotypes

Descrizione del progetto

Approfondimenti metabolici per l’immunoterapia

Il metabolismo è fondamentale per il buon funzionamento di tutte le cellule, in quanto determina la produzione di energia. Nelle cellule immunitarie, ciò si traduce in compiti quali l’uccisione delle cellule tumorali. L’ipotesi di lavoro del progetto 4Dplus_Metaflux, finanziato dal CER, è che l’efficacia delle immunoterapie dipenda dal metabolismo cellulare in siti patologici come i tumori solidi, dove lo stress metabolico può ostacolare la funzione delle cellule immunitarie. I ricercatori si baseranno su un nuovo test che misura l’assorbimento dei nutrienti con una risoluzione a livello di singola cellula. Questo test può aiutare a misurare tre sostanze nutritive nelle cellule immunitarie nei siti delle malattie e fornire informazioni fondamentali per migliorare le terapie immunitarie.

Obiettivo

The development of better immunotherapies is being hammered by our lack of understanding about immune cell metabolism in vivo at the sites of disease. We know that immune cell metabolism is pivotal in controlling immune function. For instance, disrupting Natural Killer cell metabolism prevents them from killing cancer cells. Cellular immunotherapies for diseases like solid cancer are underperforming and one central reason for this is the metabolic pressure exerted on immune cells in solid tumours. The core problem is that we cannot currently measure the metabolism of immune cells at the site of disease. As a result, the biopharma industry is ill equipped to design immmunotherapies that are effective at these sites in the face of the metabolic stress.
My ERC-CoG project (DC_Nutrient) has developed a new type of nutrient uptake assay with single cell resolution that can be used to probe metabolism in vivo. This is highly informative because the uptake of nutrients is the first limiting step for cellular metabolism. The core advance in our approach is to use bioorthogonal chemistry to attach a fluorophore to the nutrient after it has been transported into the cell. This bypasses all the pitfalls and failings of previous attempts to develop such assays using fluorophore tags.
This PoC will advance this technology to simultaneously measure the uptake of 3 separate nutrients into immune cells at the site of disease, all with single cell resolution. Combined with an in vivo measurement of protein translation this will provide 4 dimensions of metabolic flux analysis for each cell analysed by multiparmetric flow cytometry. This innovative idea will provide a licensable assay technology that will be of high value to the biopharma industry as it will enable preclinical mouse studies to gain a detailed understanding of the metabolic changes occurring in immune cells at the site of disease. This technology will drive innovation towards metabolically enhanced immunotherapies.

Campo scientifico (EuroSciVoc)

CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP. Cfr.: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

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Istituzione ospitante

THE PROVOST, FELLOWS, FOUNDATION SCHOLARS & THE OTHER MEMBERS OF BOARD, OF THE COLLEGE OF THE HOLY & UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN
Contributo netto dell'UE
€ 150 000,00
Indirizzo
COLLEGE GREEN TRINITY COLLEGE
D02 CX56 Dublin
Irlanda

Mostra sulla mappa

Regione
Ireland Eastern and Midland Dublin
Tipo di attività
Higher or Secondary Education Establishments
Collegamenti
Costo totale
Nessun dato

Beneficiari (1)