Descripción del proyecto
Clones de las células cancerosas y biología de los tumores
Las células cancerosas sufren mutaciones genéticas y se diversifican con el tiempo, dando lugar a la aparición de diferentes subpoblaciones o clones dentro de un tumor. Esta diversificación genética refuerza la progresión de los tumores y la metástasis, así como la aparición de la resistencia al tratamiento. Comprender la evolución clonal es crucial para desarrollar tratamientos neoplásicos más eficaces. Financiado por el Consejo Europeo de Investigación, el equipo del proyecto SpaceClones estudia las interacciones celulares dentro de los tumores mediante técnicas de ingeniería celular y de diagnóstico por la imagen. La idea es desentrañar los mecanismos moleculares que rigen los comportamientos clonales y descifrar los patrones espaciales, lo que permitirá obtener conocimientos importantes sobre el papel de los clones de las células cancerosas en la biología de los tumores.
Objetivo
Clonal evolution in tumours, the process by which cancer cells expand, diversify and are selected, is a major determinant of tumour growth and response to therapy. How the co-existence of diverse cancer cell clones shape tumour development is a fundamental question that remains open, in part because of limitations on existing experimental platforms and analytical frameworks. Our expertise inferring multicellular behaviours within tissue microenvironments, together with recent advances in imaging, assay automation, and cell engineering, timely place us in an excellent position to profile and engineer clonal interactions within tumours from the tissue level down to the molecular scale, which enables targeting this important question with unprecedented throughput and spatial resolution. In SpaceClones, we aim to: (1) characterize clonal interactions in genetically engineered tumours at sub-100 nm resolution, (2) characterize clonal signatures under metabolically defined environmental conditions, and (3) examine the cell state of engineered clonal spatial patterns. To overcome a variety of challenges to understanding molecular and cellular mechanisms of clonal behaviours in tumours, I have designed a ground-breaking approach that combines highly-multiplexed imaging, in vitro and in vivo tumour models, cell engineering, super-resolution microscopy, combinatorial low-volume liquid handling, and algorithms for deconstruction of spatial patterns. Altogether, SpaceClones will exemplify how to imply causality on the emergence of clonal spatial patterns in tumours, having far-reaching implications for the study of any other multicellular system. Ultimately, a deeper understanding of clonal evolution will contribute in the design of more effective cancer therapies and tools to predict clinical outcomes.
Ámbito científico
Palabras clave
Programa(s)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Régimen de financiación
HORIZON-ERC - HORIZON ERC GrantsInstitución de acogida
08028 Barcelona
España