Projektbeschreibung
Krebszellklone und Tumorbiologie
Krebszellen durchlaufen genetischen Mutationen und diversifizieren sich im Laufe der Zeit, was zur Entstehung verschiedener Subpopulationen oder Klone innerhalb eines Tumors führt. Diese genetische Diversifizierung verstärkt das Fortschreiten des Tumors und die Metastasierung sowie die Entstehung von Therapieresistenzen. Das Verständnis der klonalen Evolution ist für die Entwicklung wirksamerer Krebsbehandlungen entscheidend. Ziel des vom Europäischen Forschungsrat finanzierten Projekts SpaceClones ist es, die Zellinteraktionen innerhalb von Tumoren mithilfe von Zelltechniken und bildgebenden Verfahren zu untersuchen. Die Idee besteht darin, molekulare Mechanismen zu entschlüsseln, die das klonale Verhalten steuern, und räumliche Muster zu verstehen, die wichtige Einblicke in die Rolle von Krebszellklonen in der Tumorbiologie bieten.
Ziel
Clonal evolution in tumours, the process by which cancer cells expand, diversify and are selected, is a major determinant of tumour growth and response to therapy. How the co-existence of diverse cancer cell clones shape tumour development is a fundamental question that remains open, in part because of limitations on existing experimental platforms and analytical frameworks. Our expertise inferring multicellular behaviours within tissue microenvironments, together with recent advances in imaging, assay automation, and cell engineering, timely place us in an excellent position to profile and engineer clonal interactions within tumours from the tissue level down to the molecular scale, which enables targeting this important question with unprecedented throughput and spatial resolution. In SpaceClones, we aim to: (1) characterize clonal interactions in genetically engineered tumours at sub-100 nm resolution, (2) characterize clonal signatures under metabolically defined environmental conditions, and (3) examine the cell state of engineered clonal spatial patterns. To overcome a variety of challenges to understanding molecular and cellular mechanisms of clonal behaviours in tumours, I have designed a ground-breaking approach that combines highly-multiplexed imaging, in vitro and in vivo tumour models, cell engineering, super-resolution microscopy, combinatorial low-volume liquid handling, and algorithms for deconstruction of spatial patterns. Altogether, SpaceClones will exemplify how to imply causality on the emergence of clonal spatial patterns in tumours, having far-reaching implications for the study of any other multicellular system. Ultimately, a deeper understanding of clonal evolution will contribute in the design of more effective cancer therapies and tools to predict clinical outcomes.
Wissenschaftliches Gebiet
Schlüsselbegriffe
Programm/Programme
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Thema/Themen
Finanzierungsplan
HORIZON-ERC - HORIZON ERC GrantsGastgebende Einrichtung
08028 Barcelona
Spanien