Descripción del proyecto
Comprender el papel de las células endoteliales en el envejecimiento a nanoescala
El conocimiento a nanoescala de las células endoteliales (CE) y de su papel básico en la transferencia de metabolitos y fármacos a órganos vitales, así como su eliminación, es limitado, sobre todo en lo que respecta a su cambio con el envejecimiento. Los nanoscopios ópticos actuales son lentos y solo se pueden utilizar con células aisladas, normalmente fijadas (es decir, muertas). Además, los investigares noveles de este campo deben familiarizarse con posibles problemas como, por ejemplo, los artefactos relacionados con la reconstrucción de imágenes. En el proyecto ImAge-D, financiado con fondos europeos, se pretende eliminar estas limitaciones formando a diez estudiantes de doctorado en herramientas de imagen biomédica de alta velocidad y resolución. Se estudiarán CE vivas y sus cambios con el envejecimiento, centrándose en conceptos/habilidades de física y biomedicina en imágenes de superresolución, reconstrucción analítica de imágenes y micromanipulación óptica.
Objetivo
ImAge-D will train a new generation of Doctoral Candidates (DCs) in the development and application of newly developed high speed and high-resolution imaging tools in biomedical research. The ten DCs will be cross-pollinated with concepts and skills in physics and biomedicine, in particular in super-resolution imaging, analytical image reconstruction, and optical micro-manipulation methods. These skills will be applied to reveal for the first time the functionality and morphology (below the diffraction limit of light) of living endothelial cells (EC) that present the main barrier between the blood/lymph and all organs and tissues, and how these vital cells change with ageing. Very little is known at the nanoscale about extremely important physiological functions of EC and their role in the transfer and/or clearance of metabolites and pharmaceuticals to vital organs, and how EC change with ageing. The current generation of optical nanoscopes, however, is rather slow and can only be applied to isolated, typically fixed (i.e. dead) cells rather than biomedically relevant tissues. Also, newcomers to the field need to familiarize themselves with a whole new set of potential problems that might arise in the use of optical nanoscopy, such as image reconstruction- related artifacts to name just one example.This is an area of research where European enterprises are very active. Excellent training in new scientific and complementary skills, combined with international and intersectoral work experience, will instill an innovative, creative and entrepreneurial mind-set in ImAge-D's DCs, maximising economic benefits based on scientific discoveries. These specialised, highly trained, and mobile DCs will have greatly enhanced career prospects. The training in novel physical methods with highly relevant experience in the biomedical sciences will allow them to confidently navigate at the interface of academic, clinical and private sector research.
Ámbito científico
Palabras clave
Programa(s)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Régimen de financiación
HORIZON-TMA-MSCA-DN - HORIZON TMA MSCA Doctoral NetworksCoordinador
9019 Tromso
Noruega