Description du projet
Comprendre le rôle de la cellule endothéliale dans le vieillissement à l’échelle nanométrique
Notre compréhension des cellules endothéliales (CE) et de leur rôle crucial dans le transfert et l’élimination des métabolites et des produits pharmaceutiques vers les organes vitaux, en particulier la façon dont les CE changent avec le vieillissement, est limitée à l’échelle nanométrique. Les nanoscopes optiques actuels sont lents et ne peuvent être utilisés que sur des cellules isolées, généralement fixes (c’est-à-dire mortes). En outre, les nouveaux venus dans le domaine doivent se familiariser avec les problèmes potentiels tels que les artefacts liés à la reconstruction d’images. Le projet ImAge-D, financé par l’UE, vise à éliminer ces limitations en formant dix doctorants aux outils d’imagerie biomédicale à grande vitesse et à haute résolution. Le projet étudiera les CE vivantes et leurs changements avec le vieillissement, en se concentrant sur les concepts/compétences de la physique et de la biomédecine dans l’imagerie de super-résolution, la reconstruction analytique d’images et la micro-manipulation optique.
Objectif
ImAge-D will train a new generation of Doctoral Candidates (DCs) in the development and application of newly developed high speed and high-resolution imaging tools in biomedical research. The ten DCs will be cross-pollinated with concepts and skills in physics and biomedicine, in particular in super-resolution imaging, analytical image reconstruction, and optical micro-manipulation methods. These skills will be applied to reveal for the first time the functionality and morphology (below the diffraction limit of light) of living endothelial cells (EC) that present the main barrier between the blood/lymph and all organs and tissues, and how these vital cells change with ageing. Very little is known at the nanoscale about extremely important physiological functions of EC and their role in the transfer and/or clearance of metabolites and pharmaceuticals to vital organs, and how EC change with ageing. The current generation of optical nanoscopes, however, is rather slow and can only be applied to isolated, typically fixed (i.e. dead) cells rather than biomedically relevant tissues. Also, newcomers to the field need to familiarize themselves with a whole new set of potential problems that might arise in the use of optical nanoscopy, such as image reconstruction- related artifacts to name just one example.This is an area of research where European enterprises are very active. Excellent training in new scientific and complementary skills, combined with international and intersectoral work experience, will instill an innovative, creative and entrepreneurial mind-set in ImAge-D's DCs, maximising economic benefits based on scientific discoveries. These specialised, highly trained, and mobile DCs will have greatly enhanced career prospects. The training in novel physical methods with highly relevant experience in the biomedical sciences will allow them to confidently navigate at the interface of academic, clinical and private sector research.
Champ scientifique
Mots‑clés
Programme(s)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Régime de financement
HORIZON-TMA-MSCA-DN - HORIZON TMA MSCA Doctoral NetworksCoordinateur
9019 Tromso
Norvège