Project description
Insight into chromosomal instability in cancer
Chromosomal instability (CIN) is a key driver of cancer development, progression and resistance to therapy. CIN is associated with changes in chromosome number and structure caused by errors during cell division, DNA damage and other factors such as oxidative stress. Recent research suggests that CIN also affects how cancer cells interact with the immune system, but the exact mechanisms remain unclear. The ERC-funded CINbyFUNseq project aims to uncover these mechanisms by creating highly sensitive methods to track CIN at the single-cell level. Using advanced sequencing and spatial profiling technologies, researchers will map where and how CIN happens within cells and identify the molecular pathways involved. Project findings will deepen our understanding of CIN and its role in tumour immunology.
Objective
Chromosomal instability (CIN) is a main cause of tumor heterogeneity, tumor evolution and therapy resistance of cancers. Chromosomally instable gastric cancer (CIN-gastric cancer) accounts for 50-70% of gastric tumors and is the poorest prognosis tumor subtype with the most suppressed tumor immune microenvironment. However, it is not understood how CIN contributes to poor prognosis and edits the tumor immune response of CIN-gastric cancer, due to a lack of assays investigating native CIN-driving mechanisms at the single cell level.
I invented a pioneering, microscopy-based functional single cell sequencing method, which enables selective profiling of rare subpopulations of cells displaying dynamic phenotypes of interest. In this proposal, I will use my expertise to develop novel advanced single cell and multiplexed sequencing methods and create a new research line with the aim of dissecting the driving mechanisms of CIN in CIN-gastric cancer, investigating the impact of CIN on tumor microenvironment, and elucidating the molecular pathways leading to the poor prognosis of CIN-gastric cancer. To fulfill this aim, I defined three research objectives:
In Objective I, I will develop a deep learning-based functional single cell sequencing (deepFUNseq) method to identify and profile rare, naturally occurring CIN-mother and -daughter cells derived from CIN-gastric cancer patients.
In Objective II, I will develop a multiplexed functional & spatial single cell sequencing method(multi-FUNseq) to transcriptomically profile (non-)CIN gastric cancer cells and their interacting immune cells.
In Objective III, I will investigate CIN-driving mechanisms and CIN-associated consequences in CIN-gastric cancer, including the role of CIN in immunoediting.
The technologies developed in this proposal will result in a leap in our understanding of CIN in the context of cancer, and will lead to advances in fields where profiling cell-cell interactions is important, like tumor immunology.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine immunology
- medical and health sciences clinical medicine oncology
- medical and health sciences medical biotechnology cells technologies
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
-
HORIZON.1.1 - European Research Council (ERC)
MAIN PROGRAMME
See all projects funded under this programme
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-ERC - HORIZON ERC Grants
See all projects funded under this funding scheme
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2024-COG
See all projects funded under this callHost institution
Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
3015 GD Rotterdam
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.