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ALK-driven cancers: a Portfolio of ONcoimmunoTherapies to achieve tumor Eradication

Project description

Targeting residual ALK-positive cancer cells

The anaplastic lymphoma kinase (ALK) gene produces a receptor protein which is important for nervous system development and can be aberrantly expressed by certain cancers. When mutated or fused, ALK drives uncontrolled cell growth, causing some lung cancers, lymphomas, neuroblastomas, and other malignancies. ALK tyrosine kinase inhibitors (TKIs) can initially shrink tumours and maintain minimal residual disease (MRD), but relapse is common and prognosis poor. The ERC-funded A-PONTE project aims to eliminate residual ALK-positive cells before relapse using ALK-directed T cell immunotherapies. CAR-T cells target tumour-surface ALK, while TCR-T cells recognise peptides generated by intracellular ALK. Combined with vaccines or other methods, they may clear MRD during remission, offering potential cures.

Objective

Cancers driven by the oncogenic activation of the ALK gene include subsets of non-small cell lung cancer, lymphoma, neuroblastoma, melanoma, glioblastoma, thyroid cancer, stromal tumors and other hematologic malignancies and solid tumors. Typically, these cancers affect children or young adults in healthy conditions with a long expectancy of life. They are currently treated with ALK tyrosine kinase inhibitors (TKIs) either in first line or at relapse after other standard therapies. ALK TKIs reduce tumor burden dramatically, and often induce a prolonged state of minimal residual disease (MRD) resulting in several years of extended survival. Eventually, most ALK+ tumors become resistant to ALK TKIs by different mechanisms. Relapses occur in >90% of patients that are then left without efficacious therapies and face a dismal prognosis. This common pattern of evolution of ALK+ tumors, characterized by a prolonged state MRD before the fatal relapse, provides a window of opportunity to develop therapies that might be curative should ALK+ persister cells be fully eradicated.
In this proposal, we will develop, test and validate a portfolio of ALK-directed T cell immunotherapies to achieve eradication of residual ALK+ cells during the MRD state induced by ALK TKIs. These immunotherapies include potentiated ALK.CAR-T cells for tumors that express ALK on the surface, and ALK.TCR-T cells for tumors that express ALK internally in the cytoplasm. ALK-directed T cell immunotherapies will be tested alone or in novel modalities of combination between them, with ALK vaccines or with other approaches to further potentiate their anti-tumoral efficacy. We will empower these ALK immunotherapies to eradicate MRD based on the ALK genetic alteration that is specific to each tumor. We envision that the combination of ALK TKIs with ALK-directed T cell immunotherapies could achieve a complete cure in a large subset of patients with ALK+ tumors before the onset of relapse or metastatic spread.

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2024-ADG

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Host institution

UNIVERSITA DEGLI STUDI DI TORINO
Net EU contribution

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€ 2 497 392,00
Address
VIA GIUSEPPE VERDI 8
10124 TORINO
Italy

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Region
Nord-Ovest Piemonte Torino
Activity type
Higher or Secondary Education Establishments
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Total cost

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