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AAV9-tRNA-Gly: Towards a first effective therapy for Charcot-Marie-Tooth peripheral neuropathy

Project description

Gene therapy targets Charcot-Marie-Tooth disease

Charcot-Marie-Tooth (CMT) disease encompasses a group of inherited nerve disorders affecting motor and sensory neurons primarily in the feet, legs, hands, and arms. Typically identified in adolescence, progression continues throughout the patient’s life. There is no effective pharmacological treatment for CMT. Building on their groundbreaking work identifying the molecular mechanism underlying CMT caused by mutations in the gene coding glycyl-tRNA synthetase, the ERC-funded tRNAHigh project aims to advance a gene therapy to elevate glycyl-tRNA expression levels. The team plans to identify the optimal viral vector for vector-mediated gene transfer, and the optimal administration route and dose. The project also plans to secure personnel and funding for its spin-out company and obtain a patent for the optimal gene therapy vector.

Objective

Charcot-Marie-Tooth (CMT) disease is the most common inherited neuromuscular disease,
characterized by selective degeneration of peripheral motor and sensory neurons, leading to
progressive muscle weakness and wasting, and sensory dysfunction. Currently, no effective,
FDA/EMA-approved therapies are available for CMT, necessitating life-long symptomatic treatment.

We recently identified the molecular mechanism underlying CMT caused by mutations in glycyl-tRNA
synthetase (GlyRS). We showed that transgenic overexpression of the glycyl-transfer RNA (tRNA-Gly)
fully rescues peripheral neuropathy in two CMT-GlyRS mouse models. Furthermore, we present
preliminary data showing that viral vector-mediated gene transfer of tRNA-Gly administered to newborn,
presymptomatic CMT-GlyRS mice fully rescues their peripheral neuropathy phenotypes.

In this project we will optimize tRNA-Gly gene therapy, including identification of the optimal viral vector,
administration route, and dose to target adult motor and sensory neurons. Next to that, the project will
focus on the development of a business roadmap for XtRNA Bio, the spin-out company that I recently
co- founded with NLC Health Ventures. XtRNA Bio intends to raise significant pre-seed funding and
recruit presonnel, including an experienced CEO who will secure additional funds for continued
preclinical and clinical development of tRNA-Gly gene therapy. Finally, once identified, we will patent
the optimal gene therapy construct.

In all, this proposal constitutes a crucial step towards a first effective therapy for CMT-GlyRS and CMT
in general.

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Topic(s)

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Funding Scheme

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HORIZON-ERC-POC - HORIZON ERC Proof of Concept Grants

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Call for proposal

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(opens in new window) ERC-2024-POC

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Host institution

STICHTING RADBOUD UNIVERSITEIT
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 150 000,00
Address
HOUTLAAN 4
6525 XZ Nijmegen
Netherlands

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Region
Oost-Nederland Gelderland Arnhem/Nijmegen
Activity type
Higher or Secondary Education Establishments
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Total cost

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No data

Beneficiaries (1)

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