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Single Cell PTSD: Novel Target Identification

Project description

Understanding PTSD for better treatment

Post-traumatic stress disorder (PTSD) affects around 4 % of people worldwide. Treatment options are limited, and research has been constrained by challenges such as small sample sizes, slowing progress in understanding the condition. The ERC-funded sc-PTSD project aims to explore PTSD pathology using single-nuclei RNA sequencing, DNA methylation profiling, and 3D chromatin structure analysis. It aims to identify cellular and transcriptional changes in post-mortem brains, uncover molecular targets, and characterise traditional PTSD models through genomics. The project will also develop a novel humanised model for PTSD, test the efficacy of new and existing targets to reduce PTSD in mice, and ultimately enhance our understanding of PTSD for better interventions.

Objective

Post-traumatic stress disorder (PTSD) is a devastating psychiatric disease triggered by severe psychological trauma, affecting 4% of the global population - a prevalence projected to rise due to ongoing conflicts like the war in Ukraine and the looming threat of climate change. Despite its widespread impact, we lack effective treatments of PTSD, placing a significant burden on individuals and society.

Previous PTSD research was hindered by limitations such as bulk tissue analyses, a lack of epigenetic focus, or small sample sizes. These drawbacks limit our understanding of cell-type-specific alterations during PTSD. My proposed project will leverage single-nuclei RNA sequencing, DNA methylation profiling, and 3D chromatin structure analysis to achieve unprecedented insights into PTSD pathology.

Specifically, I aim to:

1) Identify cellular, epigenetic, and transcriptional perturbations in a post-mortem human brain PTSD cohort, revealing promising molecular targets.

2) Characterize classical models of PTSD using single-nuclei genomics, and develop novel humanized model of PTSD. This will enable the identification of the most relevant model for human PTSD, and streamline further validation of targets.

3) Test the efficacy of novel as well as already identified targets from my pilot animal study by utilizing epigenome-modifying tools to alleviate PTSD pathology in mouse models.

This project will deepen our understanding of PTSD at unprecedented resolution, ultimately validating novel molecular targets and paving the way for more effective interventions in future.

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Topic(s)

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2025-STG

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Host institution

SIEC BADAWCZA LUKASIEWICZ - PORT POLSKI OSRODEK ROZWOJU TECHNOLOGII
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 403 931,00
Address
UL STABLOWICKA 147
54 066 WROCLAW
Poland

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Region
Makroregion południowo-zachodni Dolnośląskie Miasto Wrocław
Activity type
Research Organisations
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 403 931,00

Beneficiaries (1)

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