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DamAged memories: organelle heterogeneity en route to T cell diversity

Project description

Organelle ageing, organism ageing and dysfunctional T cell differentiation

T cells are essential to the adaptive immune system, protecting against infections and even cancer. Ageing impairs the maintenance of long-lived naive stem cells (cells in an inactivated, non-dividing state), whose attrition leads to a less diverse T cell population and the accumulation of ‘terminally differentiated’ T cells. This diminishes the immune response to threats. The ERC-funded DAMAGED project will investigate the hypothesis that the ‘aged’ pools of inherited mitochondria and peroxisomes – organelles with critical roles in cell metabolism – are related to dysfunctional T cell differentiation in ageing. Beyond insight into immunology, project research findings could have impact across multiple fields in relation to stem cell-based tissue regeneration.

Objective

Maintenance of long-lived and quiescent stem cells is impaired during ageing, which negatively impacts tissue regeneration by restricting cell diversity. In the context of T lymphocytes, ageing is linked to profound changes in their repertoire, which becomes less diverse due to naïve T cell attrition. This leads to the accumulation of terminally differentiated cells and results in poorer immune responses. I aim to understand whether T cell diversification is fostered by the unequal inheritance of heterogeneous pools of organelles with distinct chronological ages. I will focus on mitochondria and peroxisomes, as T cell metabolism is key to cell fate decisions. I hypothesize that i) organelle inheritance is influenced by early events following T cell activation, ii) organelle ageing leads to changes in trafficking and function and iii) the organelle repertoire can be manipulated to rejuvenate ageing T cells. To test these hypotheses, we will: (1) Investigate whether unequal inheritance of aged vs. young organelles is determined by pre-mitotic events and the consequences of perturbing organelle trafficking; (2) Dissect whether the role of organelle fission in regulating mitochondrial and peroxisomal dynamics discriminates organelles by age; (3) Develop tools to rejuvenate cells that accumulate damage and dysfunctional cargo by targeting organelle inheritance. All aims will address the potential reciprocal relationship between organismal ageing and organelle ageing as drivers of impaired T cell stemness. The focus on ageing relies on the potential to use any discovered modulation strategies in T cell therapies, being older adults particularly vulnerable to pathologies linked to immune and metabolic disorders. These findings will also have impact beyond the field of immunology, as organelle ageing is a general cell biological phenomenon and relevant across different stem-like cell types, which can have broader implications in the perspective of tissue regeneration.

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2025-STG

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Host institution

UNIVERSITAT BASEL
Net EU contribution

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€ 1 499 672,00
Address
PETERSPLATZ 1
4051 Basel
Switzerland

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Region
Schweiz/Suisse/Svizzera Nordwestschweiz Basel-Stadt
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 499 672,50

Beneficiaries (1)

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