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Bioconversion of Coffee and Olive Wastes via Food-Grade Microbial Co-cultures for Production of Proteins, Antioxidants, and Biosurfactants in a Single Process

Project description

Microbial biorefinery for converting vegetal wastes into food-grade products

Spent coffee grounds (SCGs) and olive mill waste (OMW) are two of the most abundant agro-industrial by-products worldwide. They also pose environmental risks while offering valuable biomolecules. Although rich in proteins, lipids and phenolic compounds, current recovery methods are fragmented and limited in scalability. Supported by the Marie Skłodowska-Curie Actions programme, the GRASP project will create a food-grade microbial biorefinery using a co-culture of Saccharomyces cerevisiae and Yarrowia lipolytica to convert SCG and OMW into proteins, biosurfactants and antioxidants. Recovery methods will include ultrasound extraction and membrane separation, while stabilisation techniques will enhance product bioaccessibility. The resulting ingredients will be validated in various food systems, supporting the EU’s Green Deal and sustainable food initiatives.

Objective

Agro-industrial residues represent a major environmental and economic challenge, yet also a valuable source of functional biomolecules. Among them, spent coffee grounds (SCG) and olive mill waste (OMW) are produced in millions of tons annually in Europe, generating risks of eutrophication, greenhouse gas emissions, and soil pollution. These residues are rich in proteins, lipids, and phenolic compounds, making them promising candidates for sustainable upcycling. However, current valorization strategies are fragmented, typically recovering single compounds through solvent-intensive or monoculture-based methods, with limited scalability and economic feasibility. Here we propose a food-grade microbial biorefinery (GRASP) that integrates microbial co-cultures, green recovery processes, and food technology validation to address this gap. A co-culture of Saccharomyces cerevisiae and Yarrowia lipolytica will be optimized to simultaneously convert sugar- and lipid-rich fractions of SCG and OMW into proteins, biosurfactants, and antioxidants. Residue-derived phenolics and microbial antioxidants will be characterized by HPLC-MS/MS and complementary antioxidant assays, while proteins and biosurfactants will be recovered using ultrasound-assisted extraction, membrane separation, foam fractionation, and mild acid precipitation. Stabilization strategies, including spray-drying, alginate encapsulation, and co-precipitation, will generate multifunctional complexes with improved stability and bioaccessibility. The recovered fractions will then be validated in model food and nutraceutical systems (emulsions, oils, beverages) by assessing oxidative stability, emulsification, and nutritional enrichment. This integrated approach provides the first demonstration of multi-product recovery from SCG and OMW, creating scalable, clean-label ingredients that support EU Green Deal and Farm to Fork goals for sustainable food systems.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2025-PF

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Coordinator

UNIVERSIDAD DE GRANADA
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 194 074,56
Address
CUESTA DEL HOSPICIO SN
18071 Granada
Spain

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Region
Sur Andalucía Granada
Activity type
Higher or Secondary Education Establishments
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Total cost

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Partners (1)