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Single-molecule Nanopore Analysis Platform for RNA Readout

Obiettivo

RNA viruses, including Zika, SARS-CoV-2, and Hepatitis C, impose a profound global health burden, causing millions of deaths every year. The extraordinary rates of mutations and chemical modifications in these RNA-based pathogens enable rapid adaptation and, ultimately, immune evasion. Current detection technologies fail to capture the molecular heterogeneity that drives viral evolution, relying on ensemble averaging, biased enzymatic amplification, or computational inference to compensate for low signal-to-noise. SNAPRR aims to overcome these limitations by developing a unified single-molecule platform that identifies viral RNAs and their underlying mutations, and chemical modifications, revealing the hidden chemical diversity that shapes infectivity and pathogenesis.

SNAPRR harnesses three emerging technologies to sensitively and specifically probe viral RNA chemistry. 1) Solid-state nanopores translate individual RNA molecules into recognisable ionic current signatures, enabling rapid, quantitative readout. 2) Programmable nucleic acid nanotechnology uniquely barcodes viral RNAs for multiplexed detection, and 3) Chemoselective labelling amplifies signals from single-nucleotide mutations and chemical modifications for direct nanopore localisation.

By revealing strand-to-strand heterogeneity, SNAPRR will redefine our understanding of RNA virus biology, inform next-generation diagnostics, and provide a versatile framework for antiviral development and RNA vaccine quality control. Bridging chemistry, nanotechnology, and biophysics, this project combines fundamental insight with practical impact, creating a portable, amplification-free approach to tackle the global challenge of rapidly evolving RNA viruses.

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Parole chiave

Programma(i)

Argomento(i)

Meccanismo di finanziamento

HORIZON-ERC - HORIZON ERC Grants

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(si apre in una nuova finestra) ERC-2026-STG

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Istituzione ospitante

THE PROVOST, FELLOWS, FOUNDATION SCHOLARS & THE OTHER MEMBERS OF BOARD, OF THE COLLEGE OF THE HOLY & UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN
Contributo netto dell'UE
€ 1 670 246,00
Indirizzo
COLLEGE GREEN TRINITY COLLEGE
D02 CX56 Dublin
Irlanda
Regione
Ireland Eastern and Midland Dublin
Tipo di attività
Higher or Secondary Education Establishments
Collegamenti
Costo totale
€ 1 670 246,00

Beneficiari (1)