Objective
The PolyModE project convenes an international, interdisciplinary, and intersectorial consortium to identify, characterise, and optimise novel polysaccharide modifying enzymes, and to develop robust fermentation strategies for their large-scale production, to exploit the potential of biopolymers for food, pharmaceutical, cosmetic, and technical applications. We have selected the six complex carbohydrates with the highest current market share or expected future market potential, namely alginate, carrageenan, chitosan, glycosaminoglycan, pectin, and xanthan gum. For each of these, the industrial partners have identified those enzymes which will answer to the most pressing needs or offer the most promising potential for improved production of polysaccharides with novel physico-chemical properties and biological functionalities. Primary targets will be alginate epimerases, carrageenan sulfatases, chitosan de-acetylases, glycosaminoglycan sulfatases, pectin de-acetylases, and xanthan gum de-acetylases. These enzymes together with secondary target enzymes, e.g. sequence specific lyases and hydrolases, will allow the generation and analysis of polymers and oligomers with novel, non-random patterns of modification. Two parallel approaches will be followed for each type of polysaccharide modifying enzyme, namely a knowledge-based genomic approach and a broad, un-biased metagenomic approach, e.g. using soil or sludge samples with a history of contact with the polysaccharide in question. A pipeline of three levels of fermentation systems will be established, ranging from lab-scale innovative expression systems with features shaped according to the specific characteristics of our target enzymes, through medium-scale, novel and unusual fermentation systems provided by a number of SME with highly specialised knowledge and expertise in developing and using such systems, to the established large-scale fermentation systems and facilities of market leaders in White Biotechnology.
Fields of science
- humanitieshistory and archaeologyhistory
- natural scienceschemical sciencespolymer sciences
- natural sciencesbiological sciencesbiochemistrybiomoleculescarbohydrates
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
- engineering and technologyindustrial biotechnologybioprocessing technologiesfermentation
Call for proposal
FP7-KBBE-2007-2A
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Funding Scheme
CP-IP - Large-scale integrating projectCoordinator
48149 Muenster
Germany
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Participants (14)
75007 Paris Cedex 07
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75794 Paris
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6708 PB Wageningen
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750 07 Uppsala
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1113 Sofia
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1001 Copenhagen K
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91380 Chilly Mazarin
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54100 Nancy
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93053 Regensburg
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31400 Toulouse
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40764 Langenfeld
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31681 Labege
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69616 Villeurbanne
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