Novel Polysaccharide Modifying Enzymes to Optimise the Potential of Hydrocolloids for Food and Medical Applications

Objective

The PolyModE project convenes an international, interdisciplinary, and intersectorial consortium to identify, characterise, and optimise novel polysaccharide modifying enzymes, and to develop robust fermentation strategies for their large-scale production, to exploit the potential of biopolymers for food, pharmaceutical, cosmetic, and technical applications. We have selected the six complex carbohydrates with the highest current market share or expected future market potential, namely alginate, carrageenan, chitosan, glycosaminoglycan, pectin, and xanthan gum. For each of these, the industrial partners have identified those enzymes which will answer to the most pressing needs or offer the most promising potential for improved production of polysaccharides with novel physico-chemical properties and biological functionalities. Primary targets will be alginate epimerases, carrageenan sulfatases, chitosan de-acetylases, glycosaminoglycan sulfatases, pectin de-acetylases, and xanthan gum de-acetylases. These enzymes together with secondary target enzymes, e.g. sequence specific lyases and hydrolases, will allow the generation and analysis of polymers and oligomers with novel, non-random patterns of modification. Two parallel approaches will be followed for each type of polysaccharide modifying enzyme, namely a knowledge-based genomic approach and a broad, un-biased metagenomic approach, e.g. using soil or sludge samples with a history of contact with the polysaccharide in question. A pipeline of three levels of fermentation systems will be established, ranging from lab-scale innovative expression systems with features shaped according to the specific characteristics of our target enzymes, through medium-scale, novel and unusual fermentation systems provided by a number of SME with highly specialised knowledge and expertise in developing and using such systems, to the established large-scale fermentation systems and facilities of market leaders in White Biotechnology.

Field of science

/social sciences/economics and business/business and management/commerce
/natural sciences/biological sciences/biochemistry/biomolecules/carbohydrates
/natural sciences/chemical sciences/polymer science
/engineering and technology/industrial biotechnology/bioprocessing technologies/fermentation
/humanities/history and archaeology/history
/natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes

Funding Scheme

CP-IP - Large-scale integrating project

Coordinator

Westfälische Wilhelms-Universität Münster

Address

Schlossplatz 2
48149 Muenster
Germany

Activity type

Higher or Secondary Education Establishments

EU contribution

€ 1 050 192

Objective

Field of science

Programme(s)

Topic(s)

Call for proposal

Funding Scheme

Coordinator

Participants (14)

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