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Bottom-up design and fabrication of industrial bio-inorganic nano-porous membranes with novel functionalities based on principles of protein self-assembly and biomineralization

Objectif

There is strong interest in the development of novel functionalized membranes which can be used as microsieves, as a component of integrated analytical systems, in food processing, drug discovery and diagnostic applications. This project is based on a combination of three break-through technologies, developed by the applicants in the past, with high impact for nano(bio)technological application: (i) the S-layer technology allowing the construction of nanoporous protein lattices, (ii) the biocatalytic formation of inorganic materials by silicatein, a group of unique enzymes capable to catalyze the formation of porous silica from soluble precursors, and (iii) the sol-gel technique for encapsulation (immobilization) of biomolecules serving as biocatalyst or as a component of sensors. The goal of this project is to design and fabricate - based on molecular biology inspired approaches - nano-porous bio-inorganic membranes with novel functionalities for industrial application. These membranes will be formed by S-layer proteins, which are able to assemble to highly ordered structures of defined pore-size, and recombinant silicateins or silicatein fusion proteins. The hydrated silica glass layer formed by silicatein will be used to encase biocatalysts (enzymes) or antibodies against small molecules as sample prep- or sensor components of integrated systems. The innovative type of the functionalized membranes developed in this project thus exploits two principles: (i) protein self-assembly and - and this has not been done before - (ii) enzymatic (silicatein-mediated) deposition of inorganic material used for reinforcement of the membranes as well as for encasing biomolecules, providing the membranes with new functionalities. The new technique will be exploited by three research-based SMEs and the enduser involved in the project, in microfluidics based sample processing and micro-array development, in industrial nanosieves, as well as in sensors in drinking water systems.

Appel à propositions

FP7-KBBE-2009-3
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Coordinateur

UNIVERSITAETSMEDIZIN DER JOHANNES GUTENBERG-UNIVERSITAET MAINZ
Adresse
Langenbeckstrasse 1
55131 Mainz
Allemagne
Type d’activité
Higher or Secondary Education Establishments
Contribution de l’UE
€ 630 634
Contact administratif
Werner E.G. Müller (Prof.)

Participants (7)

UNIVERSITAET FUER BODENKULTUR WIEN
Autriche
Contribution de l’UE
€ 555 180
Adresse
Gregor Mendel Strasse 33
1180 Wien
Type d’activité
Higher or Secondary Education Establishments
Contact administratif
Uwe B. Sleytr (Prof.)
WAGENINGEN UNIVERSITY
Pays-Bas
Contribution de l’UE
€ 309 400
Adresse
Droevendaalsesteeg 4
6708 PB Wageningen
Type d’activité
Higher or Secondary Education Establishments
Contact administratif
Jos Teunissen (Mr.)
UNIVERSITE PIERRE ET MARIE CURIE - PARIS 6
France
Contribution de l’UE
€ 250 000
Adresse
Place Jussieu 4
75252 Paris
Type d’activité
Higher or Secondary Education Establishments
Contact administratif
Elena Billi-Rizza (Dr.)
NANOTECMARIN GMBH
Allemagne
Contribution de l’UE
€ 245 720
Adresse
Muhlstrasse 19
55218 Ingelheim Am Rhein
Type d’activité
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Contact administratif
Jasmine Grams (Ms.)
AQUAMARIJN MICRO FILTRATION BV
Pays-Bas
Contribution de l’UE
€ 270 600
Adresse
Singel 58 B
1015 AB Amsterdam
Type d’activité
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Contact administratif
Cees J.M. Van Rijn (Prof.)
LIONIX BV
Pays-Bas
Contribution de l’UE
€ 302 685
Adresse
Hengelosestraat 500
7521 AN Enschede
Type d’activité
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Contact administratif
Henk Leeuwis (Mr.)
IWW RHEINISCH WESTFALISCHES INSTITUT FUR WASSERFORSCHUNG GEMEINNUTZIGE GMBH
Allemagne
Contribution de l’UE
€ 252 600
Adresse
Moritzstrasse 26
45476 Mulheim An Der Ruhr
Type d’activité
Research Organisations
Contact administratif
Klaus-Dieter Neumann (Mr.)