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Content archived on 2024-06-18

Mechanobiology of Aplysia neurons

Objective

Memory loss is a central symptom in different diseases such as Alzheimer’s disease, and represents a significant social and economic burden for a large percentage of European citizens. Neuronal cell adhesion molecules belonging to the immunoglobulin superfamily (IgCAMs) are known to be involved in brain development processes, and also contribute to the synaptic alterations connected with memory formation in adults. The goal of this project is to elucidate the molecular, biophysical and cellular mechanisms of directed movements of neuronal growth cones, and in particular how, upon binding to the extracellular matrix or to other cells, IgCAMs control cytoskeletal dynamics and therefore synaptic plasticity. The central hypothesis of this proposal is that adhesion-mediated growth cone guidance involves a force transduced by the cytoskeleton upon IgCAM adhesion, and that this mechanical signal further stimulates Src protein tyrosine kinase activation. In order to test this hypothesis, state-of-the-art techniques will be combined in a highly interdisciplinary manner. This project lies at the interface between mechanics, cell biology, biophysics and surface physics. The proposer will use a well-established cellular model system for growth cone studies (Aplysia), state-of-the-art molecular tools (recombinant IgCAM and Src biosensor) and a high-resolution force measurement system (Atomic Force Microscopy, AFM) coupled with FRET imaging. By applying the first molecule-specific AFM measurements to live neuronal growth cones, the proposer will measure the forces transduced by IgCAMs to the growth cone cytoskeleton and at correlating them with Src activity in real time, thereby proving the force-dependence of neuronal connectivity. This proposal is related to many of the FP7 research objectives, such as “Nanosciences, Nanotechnologies, Materials and New Production Technologies" and "Health”, specifically “Research on the Brain and Related Diseases, Human Development and Ageing”

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-2009-IEF
See other projects for this call

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN
EU contribution
€ 178 374,20
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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