Objectif Due to the emerging resistance of bacterial pathogens against commonly used drugs, there is an urgent requirement for new antibiotic compounds and lead structures. The European Commission encouraged researchers to investigate this problem in a holistic manner. Recently bacteria with complex lifestyles and intensive “crosstalk” came into focus as potential source for new small molecule lead structures which might be further developed as therapeutic agents. Planctomycetes comprise ideal candidates, since these ubiquitous occurring organisms, which reproduce via budding, form cell aggregates, such as biofilms and “marine-snow”. Beside their complex lifestyle, they comprise sub cellular compartments resembling the eukaryotic nucleus which questions the pro-/eukaryotic dichometrie. Thus, they combine a complex lifestyle with interspecies communication, which is required for biofilm formation. However, in-depth analysis of Planctomycetes secondary metabolite production has been hampered by the lack of a genetic system. Consequently the major objective of this proposal is the construction of a genetic system for Planctomycetes. Such a system would allow e.g. the construction of deletion mutants and the identification of secondary metabolites while comparing these mutants against the wild type strain. In addition the genetic system will be highly beneficial for applications beyond the proposed project, since e.g. genetic modification of Planctomycetes which play an important role in waste water treatment will result in further biotechnological applications. In addition to the development of the genetic system, the capability of small molecule synthesis will be investigate via genome mining of the already sequenced Planctomycetes species. Further more surrogate genetic experiments will allow the cloning and heterologous expression of secondary-metabolite related genes and operons, which subsequently enables small molecule structure determination and characterization. Champ scientifique engineering and technologyenvironmental engineeringwater treatment processeswastewater treatment processesnatural sciencesbiological sciencesmicrobiologybacteriologymedical and health sciencesmedical biotechnologygenetic engineeringnatural sciencesbiological sciencesgeneticsgenomes Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-2009-IOF - Marie Curie Action: "International Outgoing Fellowships for Career Development" Appel à propositions FP7-PEOPLE-2009-IOF Voir d’autres projets de cet appel Régime de financement MC-IOF - International Outgoing Fellowships (IOF) Coordinateur LEIBNIZ-INSTITUT DSMZ-DEUTSCHE SAMMLUNG VON MIKROORGANISMEN UND ZELLKULTUREN GMBH Contribution de l’UE € 238 099,90 Adresse INHOFFENSTRASSE 7B 38124 Braunschweig Allemagne Voir sur la carte Région Niedersachsen Braunschweig Braunschweig, Kreisfreie Stadt Type d’activité Research Organisations Contact administratif Heike Bauer (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Participants (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV Participation terminée Allemagne Contribution de l’UE Aucune donnée Adresse HOFGARTENSTRASSE 8 80539 Munchen Voir sur la carte Région Bayern Oberbayern München, Kreisfreie Stadt Type d’activité Research Organisations Contact administratif Heike Wojack (Ms.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée