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Assembling biomembranes: fundamentals of membrane transporter folding and creation of synthetic modules

Objective

Self-assembly is a hallmark of Biology. We are far from a complete understanding of this natural assembly, which in turn limits our ability to mimic biological construction in the bioengineering of tuneable synthetic systems.
This proposal addresses the major challenge of membrane protein folding. Here, I intend to make a step change to my pioneering biophysical studies and investigate co-translational membrane protein folding. A central feature will be the creation of synthetic systems to probe key events in co-translational folding, as the protein folds in the membrane whilst emerging from the ribosome. An ambitious target is to make these systems tuneable, which will provide a new tool for the fabrication of membrane proteins and artificial cells in Synthetic Biology. The assembly of a tuneable artificial module that affords control over membrane protein synthesis is unprecedented.
I focus on the ubiquitous superfamily of major facilitator proteins, namely the best studied family member, lactose permease, LacY. This proposal has state of the art biophysical mechanistic studies at its core, which interleave into Cell and Synthetic Biology. There are two themes:
Theme 1. Determination of fundamental membrane protein folding parameters: folding transition states and lipid control
Phi-value analysis will be used to probe the folding transition state of LacY; the first such analysis of a multi-domain membrane protein. Lipid parameters that control LacY folding will be quantified, including bilayer asymmetry using novel droplet interface bilayer methods.
Theme 2: construction of tuneable synthetic co-translational folding systems
Engineered ribosomes and translocon insertion machinery will be incorporated and LacY folding will be controlled. Translation will be regulated or halted using mutant ribosomes, arrest sequences, altered codon usage and controlling tRNA addition. Trapped LacY folding intermediates will be studied using biophysical methods.

Call for proposal

ERC-2011-ADG_20110310
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Host institution

KING'S COLLEGE LONDON
Address
Strand
WC2R 2LS London
United Kingdom
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 685 914,20
Principal investigator
Paula Jane Booth (Prof.)
Administrative Contact
Paul Labbett (Mr.)

Beneficiaries (2)

KING'S COLLEGE LONDON
United Kingdom
EU contribution
€ 1 685 914,20
Address
Strand
WC2R 2LS London
Activity type
Higher or Secondary Education Establishments
Principal investigator
Paula Jane Booth (Prof.)
Administrative Contact
Paul Labbett (Mr.)
UNIVERSITY OF BRISTOL

Participation ended

United Kingdom
EU contribution
€ 626 474,80
Address
Beacon House Queens Road
BS8 1QU Bristol
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Sarah Everett-Cox (Ms.)