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Identification of molecular pathways underlying activity-dependent neuron-glia communication using in vitro microfluidic systems

Ziel

Interactions between neurons and glial cells underlie the normal development, structure and function of the nervous system. Perturbation of this communication occurs during injury, in acquired or hereditary neuropathic diseases of the central and peripheral nervous systems, and with ageing. However, the specific nature and the physiological significance of neuron-glia interactions remain largely unknown. We propose here the use of a microfluidic co-culture platform to characterize and investigate the role of non-synaptic responses of glial cells following stimulation of neuronal activity. In particular, we will co-culture neurons and glial cells of the peripheral nervous system, and monitor glia-specific changes at the transcriptome- and the secretome-levels, after manipulation of the electrical activity of neurons. We will subsequently interfere with the identified signaling pathways, and study effects on neuronal structure and function. Following this in vitro approach, we expect to identify potential mediators of the axon-glia communication and decipher their role in maintaining the nervous system integrity and function.

Aufforderung zur Vorschlagseinreichung

FP7-PEOPLE-2011-IIF
Andere Projekte für diesen Aufruf anzeigen

Koordinator

UNIVERSITE DE LAUSANNE
EU-Beitrag
€ 184 709,40
Adresse
QUARTIER UNIL CENTRE - BATIMENT UNICENTRE
1015 LAUSANNE
Schweiz

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Region
Schweiz/Suisse/Svizzera Région lémanique Vaud
Aktivitätstyp
Higher or Secondary Education Establishments
Kontakt Verwaltung
Roman Chrast (Prof.)
Links
Gesamtkosten
Keine Daten