Objective
Ribosomes, large and dynamic particles made of RNA and proteins, are amongst the most complex macromolecular machines known to man. High resolution tools have provided exciting views of the ribosome structure. Revealing dynamics of ribosomal complexes in solution, however, has so far been challenging due to the restricted stability and homogeneity of ribosomes. A set of new tools developed by the Rappsilber lab, partly by me over the last few months, changes dramatically the feasibility of investigating ribosome dynamics. I aim to use these new mass spectrometry-based technologies to determine accurate sites and changes in yield of cross-linking between proteins and between RNA and proteins. Thereby, using protein-protein and RNA-protein cross-linking/mass spectrometry approaches, I aim to answer:
1. Is the final and rate-limiting step in the biogenesis of 50S ribosomal subunits a conformational rearrangement? This will prepare the subsequent analysis of the other steps during the biogenesis of ribosomes in E. coli and in future also in the eukaryote S. cerevisiae.
2. How does the structure and extra-ribosomal protein content of translating ribosomes change in response to different stress conditions? This will complement electron microscopy data and lead into the analysis of how translation links to virulence in pathogenic bacteria.
My project will showcase the potential of cross-linking/mass spectrometry for the study of ribonucleoprotein complexes and, indeed, for protein-nucleotide interactions in general, i.e. encompass DNA-protein interactions. Furthermore, results of this study may facilitate the development of next-generation antibacterial drugs, targeting assembly factors and conformational rearrangements during ribosome biogenesis. For my academic career, acquiring knowledge in cutting edge proteomics approaches will be a stepping stone and further strengthen my role as gatekeeper of proteomics knowledge and a catalyst of biological sciences in Estonia.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences microbiology bacteriology
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences biological sciences genetics RNA
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
EH8 9YL Edinburgh
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.