Objective
The proposed project is to use the combination of d-block and f-block units in the same dinuclear complex for parallel two-component luminescent imaging of different species in cells. This will rely on the unprecedented combination of two-photon excitation of a d-block luminophore (Pt- or Ir-based) having a long-lived excited state, followed by partial d-f energy-transfer to sensitise the lanthanide ion. This will generate two-component luminescence (blue, from the Ir/Pt unit; green or red, from the Tb or Eu unit, respectively) in which the two luminescence components have lifetimes that differ by three orders of magnitude (microsecond for d-block luminescence; millisecond for lanthanide luminescence). These different emission components can be selected at the detection stage using time gating such that purely one or the other, or a desired combination of both, can be detected in a given time window.
In addition to providing two luminescence outputs with different colours and lifetimes, each luminescence component is independently sensitive to different species in cells and can therefore be used for imaging. The lifetime of Pt or Ir-based emission, from an 3MLCT excited state, is sensitive to quenchers such as dioxygen and variations in the luminescence lifetime in different parts of a cell can be used to visualise this. In contrast the relative intensity of Eu-based emission bands varies with the concentration of chelating anions such as citrate and hydrogen carbonate, allowing ratiometric intensity-based sensing of these species. Thus these d/f complexes will for the first time allow two different species to be imaged in real time using a single molecule under two-photon excitation conditions, which would constitute a major advance in the field with clear potential applications.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology materials engineering colors
- natural sciences chemical sciences inorganic chemistry inorganic compounds
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2012-IIF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
S10 2TN SHEFFIELD
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.