Objectif The proposed project is to use the combination of d-block and f-block units in the same dinuclear complex for parallel two-component luminescent imaging of different species in cells. This will rely on the unprecedented combination of two-photon excitation of a d-block luminophore (Pt- or Ir-based) having a long-lived excited state, followed by partial d-f energy-transfer to sensitise the lanthanide ion. This will generate two-component luminescence (blue, from the Ir/Pt unit; green or red, from the Tb or Eu unit, respectively) in which the two luminescence components have lifetimes that differ by three orders of magnitude (microsecond for d-block luminescence; millisecond for lanthanide luminescence). These different emission components can be selected at the detection stage using time gating such that purely one or the other, or a desired combination of both, can be detected in a given time window.In addition to providing two luminescence outputs with different colours and lifetimes, each luminescence component is independently sensitive to different species in cells and can therefore be used for imaging. The lifetime of Pt or Ir-based emission, from an 3MLCT excited state, is sensitive to quenchers such as dioxygen and variations in the luminescence lifetime in different parts of a cell can be used to visualise this. In contrast the relative intensity of Eu-based emission bands varies with the concentration of chelating anions such as citrate and hydrogen carbonate, allowing ratiometric intensity-based sensing of these species. Thus these d/f complexes will for the first time allow two different species to be imaged in real time using a single molecule under two-photon excitation conditions, which would constitute a major advance in the field with clear potential applications. Champ scientifique engineering and technologymaterials engineeringcolorsnatural scienceschemical sciencesinorganic chemistryinorganic compounds Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-2012-IIF - Marie Curie Action: "International Incoming Fellowships" Appel à propositions FP7-PEOPLE-2012-IIF Voir d’autres projets de cet appel Régime de financement MC-IIF - International Incoming Fellowships (IIF) Coordinateur THE UNIVERSITY OF SHEFFIELD Contribution de l’UE € 231 283,20 Adresse FIRTH COURT WESTERN BANK S10 2TN Sheffield Royaume-Uni Voir sur la carte Région Yorkshire and the Humber South Yorkshire Sheffield Type d’activité Higher or Secondary Education Establishments Contact administratif Joanne Watson (Mrs.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée