Final Report Summary - MYOII-DRIVEN FISSION (Reconstitution of Myosin IIA-driven membrane fission in vitro)
The aim of this project is the development of an in vitro system to mimic the fission of vesicles from the trans-Golgi network. We have developed a system in which we can recruit Rab6A to Giant Unilamellar Vesicles (GUV) after prenylation of the protein in vitro. Several Rab6 effectors can be recruited to these GUVs in a Rab6:GTP dependent manner. Furthermore we have isolated Golgi membranes on which we were able to detect Rab6A, nmMyoIIA and actin and have pulled tubes from these membranes.