Objective
Prostate cancer affects people all over the world. The most frequent site of prostate cancer metastasis is the bone and once it has metastasised it is incurable and extremely painful. The goal of this proposal is to develop new therapeutic tools for palliative care for bone metastases. Our model states that the high levels of uPA in the bone induce cleavage of the a6 integrin in prostate cancer cells, leading to the activation of other integrins (such as a2, a3 and av) to allow for migration and homing on the bone matrix. In this proposal, we will investigate whether the a6 integrin cleavage by uPA controls cell adhesion by integrins other than the a6 integrin, a process referred to as "integrin switching".
In addition, we will investigate whether the a6 integrin cleavage by uPA controls migration through focal adhesion signalling. First, we will test the hypothesis that blocking the cleavage of the a6 integrin will reduce osteoblast conditioned media (OBCM) induced adhesion of prostate cancer cells on b one matrix. We will test this by using a peptide and an antibody designed to prevent a6 integrin cleavage and we will also perform transfections of an uncleavable a6 integrin mutant in the PC3 prostate cancer cell line. We will evaluate changes in adhesio n on different substrates (collagen, vitronectin and osteopontin) using cell adhesion assays. Second, we will test the hypothesis that blocking the cleavage (using a peptide, an antibody and transfections) of the a6 integrin will reduce the phosphorylation levels of FAK, Pax and p130CAS upon OBCM stimulation.
Third, using the peptide, the antibody and the transfections in the PC3 cell line, we will investigate the role of the a6 integrin cleavage by uPA in cell migration and invasion upon OBCM stimulation . Migration will be measured using the Cell Sedimentation Manifold (CSM), and the scratch assay, and invasion using the Boyden chamber method.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences clinical medicine oncology prostate cancer
- natural sciences earth and related environmental sciences geology sedimentology
- natural sciences biological sciences biochemistry biomolecules
- natural sciences biological sciences cell biology
- natural sciences biological sciences developmental biology
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-12
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IRG - Marie Curie actions-International re-integration grants
Coordinator
NICOSIA
Cyprus
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.