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Contenuto archiviato il 2024-05-29

Signalling and Membrane Trafficking in Transformation and Differentiation

Obiettivo

Intracellular communication in eukaryotes is largely achieved by the trafficking of membranes carrying membrane-bound and/or intravesicular signalling molecules.Inside cells, vesicles circulate from one intracellular compartment to another thus facilitating vectorial transport of signals.Between cells, membrane anchored and secreted soluble molecules mediate extracellular signalling.Exocytosis and endocytosis of receptors control the response of sensitive cells to extracellular signals.The routes of membrane trafficking are controlled by cell signalling pathways but the underlying molecular machinery is scarcely characterised. Moreover, membrane trafficking has been studied mainly in differentiated cells and rarely in cells changing phenotype during differentiation or dedifferentiation.Such drastic phenotypic changes occur during development when cells mature to differentiated cells as complex as neurons, and during malignant transformation.The goal of this STREP is to establish the connections between signalling pathways and membrane trafficking in the context of migrating, dividing and adhering mammalian cells.Through the study of membrane traffic in the course of cell differentiation, dedifferentiation, and during mitosis, we aim to unravel how important signalling pathways remodel the intracellular trafficking routes and conversely,how membrane traffic can influence signalling cascades.We will take advantage of several cellular models including neurons differentiating in culture and cancer cells dividing and migrating. We will investigate proteins that play central roles in membrane trafficking and secretion such as rabs,SNAREs and their partners. We will focus on the trafficking of signalling molecules including cell-cell and cell-substrate adhesion molecules, growth factors and their receptors, and also investigate the role of glycosylation. Through these approaches we will define the connections between Signalling and Traffic.

Campo scientifico (EuroSciVoc)

CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP. Cfr.: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

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FP6-2002-LIFESCIHEALTH
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INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE
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