Molecular analysis of the Hedgehog signal transduction complex in the primary cilium

Project Information

CilDyn

Grant agreement ID: 647278

DOI

10.3030/647278

Project closed

EC signature date 16 July 2015

Start date 1 August 2015

End date 31 January 2021

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 727 456,00

EU contribution

€ 1 727 456,00

Coordinated by

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
United Kingdom

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (22)

Cholesterol access in cellular membranes controls Hedgehog signaling

Author(s): Arun Radhakrishnan, Rajat Rohatgi, Christian Siebold
Published in: Nature Chemical Biology, Issue 16/12, 2020, Page(s) 1303-1313, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-020-00678-2

Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling

Author(s): Ross A. Robinson, Samuel C. Griffiths, Lieke L. van de Haar, Tomas Malinauskas, Eljo Y. van Battum, Pavol Zelina, Rebekka A. Schwab, Dimple Karia, Lina Malinauskaite, Sara Brignani, Marleen H. van den Munkhof, Özge Düdükcü, Anna A. De Ruiter, Dianne M.A. Van den Heuvel, Benjamin Bishop, Jonathan Elegheert, A. Radu Aricescu, R. Jeroen Pasterkamp, Christian Siebold
Published in: Cell, Issue 184/8, 2021, Page(s) 2103-2120.e31, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2021.02.045

Photochemical Probe Identification of a Small‐Molecule Inhibitor Binding Site in Hedgehog Acyltransferase (HHAT)**

Author(s): Thomas Lanyon‐Hogg, Markus Ritzefeld, Leran Zhang, Sebastian A. Andrei, Balazs Pogranyi, Milon Mondal, Lea Sefer, Callum D. Johnston, Claire E. Coupland, Jake L. Greenfield, Joshua Newington, Matthew J. Fuchter, Anthony I. Magee, Christian Siebold, Edward W. Tate
Published in: Angewandte Chemie International Edition, Issue 60/24, 2021, Page(s) 13542-13547, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202014457

RGMs: Structural Insights, Molecular Regulation, and Downstream Signaling

Author(s): Christian Siebold, Toshihide Yamashita, Philippe P. Monnier, Bernhard K. Mueller, R. Jeroen Pasterkamp
Published in: Trends in Cell Biology, Issue 27/5, 2017, Page(s) 365-378, ISSN 0962-8924
Publisher: Elsevier BV
DOI: 10.1016/j.tcb.2016.11.009

Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling

Author(s): Giovanni Luchetti, Ria Sircar, Jennifer H Kong, Sigrid Nachtergaele, Andreas Sagner, Eamon FX Byrne, Douglas F Covey, Christian Siebold, Rajat Rohatgi
Published in: eLife, Issue 5, 2016, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/eLife.20304

A calcium-sensing receptor mutation causing hypocalcemia disrupts a transmembrane salt bridge to activate β-arrestin–biased signaling

Author(s): Caroline M. Gorvin, Valerie N. Babinsky, Tomas Malinauskas, Peter H. Nissen, Anders J. Schou, Aylin C. Hanyaloglu, Christian Siebold, E. Yvonne Jones, Fadil M. Hannan, Rajesh V. Thakker
Published in: Science Signaling, Issue 11/518, 2018, Page(s) eaan3714, ISSN 1937-9145
Publisher: American Association for the Advancement of Science
DOI: 10.1126/scisignal.aan3714

Multiple ligand binding sites regulate the Hedgehog signal transducer Smoothened in vertebrates

Author(s): Eamon FX Byrne, Giovanni Luchetti, Rajat Rohatgi, Christian Siebold
Published in: Current Opinion in Cell Biology, Issue 51, 2018, Page(s) 81-88, ISSN 0955-0674
Publisher: Elsevier BV
DOI: 10.1016/j.ceb.2017.10.004

Structural basis of Smoothened regulation by its extracellular domains

Author(s): Eamon F. X. Byrne, Ria Sircar, Paul S. Miller, George Hedger, Giovanni Luchetti, Sigrid Nachtergaele, Mark D. Tully, Laurel Mydock-McGrane, Douglas F. Covey, Robert P. Rambo, Mark S. P. Sansom, Simon Newstead, Rajat Rohatgi, Christian Siebold
Published in: Nature, Issue 535/7613, 2016, Page(s) 517-522, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/nature18934

Calibration-free <em>In Vitro</em> Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Author(s): Rory Nolan, Luis A. Alvarez, Samuel C. Griffiths, Jonathan Elegheert, Christian Siebold, Sergi Padilla-Parra
Published in: Journal of Visualized Experiments, Issue 137, 2018, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/58157

Cilia-Associated Oxysterols Activate Smoothened

Author(s): David R. Raleigh, Navdar Sever, Pervinder K. Choksi, Monika Abedin Sigg, Kelly M. Hines, Bonne M. Thompson, Daniel Elnatan, Priyadarshini Jaishankar, Paola Bisignano, Francesc R. Garcia-Gonzalo, Alexis Leigh Krup, Markus Eberl, Eamon F.X. Byrne, Christian Siebold, Sunny Y. Wong, Adam R. Renslo, Michael Grabe, Jeffrey G. McDonald, Libin Xu, Philip A. Beachy, Jeremy F. Reiter
Published in: Molecular Cell, Issue 72/2, 2018, Page(s) 316-327.e5, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2018.08.034

Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins

Author(s): Jonathan Elegheert, Ester Behiels, Benjamin Bishop, Suzanne Scott, Rachel E. Woolley, Samuel C. Griffiths, Eamon F. X. Byrne, Veronica T. Chang, David I. Stuart, E. Yvonne Jones, Christian Siebold, A. Radu Aricescu
Published in: Nature Protocols, Issue 13/12, 2018, Page(s) 2991-3017, ISSN 1754-2189
Publisher: Nature Publishing Group
DOI: 10.1038/s41596-018-0075-9

Calcium-sensing receptor residues with loss- and gain-of-function mutations are located in regions of conformational change and cause signalling bias

Author(s): Caroline M Gorvin, Morten Frost, Tomas Malinauskas, Treena Cranston, Hannah Boon, Christian Siebold, E Yvonne Jones, Fadil M Hannan, Rajesh V Thakker
Published in: Human Molecular Genetics, Issue 27/21, 2018, Page(s) 3720-3733, ISSN 0964-6906
Publisher: Oxford University Press
DOI: 10.1093/hmg/ddy263

The morphogen Sonic hedgehog inhibits its receptor Patched by a pincer grasp mechanism

Author(s): Amalie F. Rudolf, Maia Kinnebrew, Christiane Kowatsch, T. Bertie Ansell, Kamel El Omari, Benjamin Bishop, Els Pardon, Rebekka A. Schwab, Tomas Malinauskas, Mingxing Qian, Ramona Duman, Douglas F. Covey, Jan Steyaert, Armin Wagner, Mark S. P. Sansom, Rajat Rohatgi, Christian Siebold
Published in: Nature Chemical Biology, Issue 15/10, 2019, Page(s) 975-982, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-019-0370-y

Cholesterol accessibility at the ciliary membrane controls hedgehog signaling

Author(s): Maia Kinnebrew, Ellen J Iverson, Bhaven B Patel, Ganesh V Pusapati, Jennifer H Kong, Kristen A Johnson, Giovanni Luchetti, Kaitlyn M Eckert, Jeffrey G McDonald, Douglas F Covey, Christian Siebold, Arun Radhakrishnan, Rajat Rohatgi
Published in: eLife, Issue 8, 2019, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.50051

Biochemical mechanisms of vertebrate hedgehog signaling

Author(s): Jennifer H. Kong, Christian Siebold, Rajat Rohatgi
Published in: Development, Issue 146/10, 2019, Page(s) dev166892, ISSN 0950-1991
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/dev.166892

Acylation-coupled lipophilic induction of polarisation (Acyl-cLIP): a universal assay for lipid transferase and hydrolase enzymes

Author(s): Thomas Lanyon-Hogg, Markus Ritzefeld, Lea Sefer, Jasmine K. Bickel, Amalie F. Rudolf, Nattawadee Panyain, Ganka Bineva-Todd, Cory A. Ocasio, Nicola O'Reilly, Christian Siebold, Anthony I. Magee, Edward W. Tate
Published in: Chemical Science, Issue 10/39, 2019, Page(s) 8995-9000, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9sc01785b

Diversity of oligomerization in Drosophila semaphorins suggests a mechanism of functional fine-tuning

Author(s): Daniel Rozbesky, Ross A. Robinson, Vitul Jain, Max Renner, Tomas Malinauskas, Karl Harlos, Christian Siebold, E. Yvonne Jones
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-11683-y

Structures of vertebrate Patched and Smoothened reveal intimate links between cholesterol and Hedgehog signalling

Author(s): Christiane Kowatsch, Rachel E Woolley, Maia Kinnebrew, Rajat Rohatgi, Christian Siebold
Published in: Current Opinion in Structural Biology, Issue 57, 2019, Page(s) 204-214, ISSN 0959-440X
Publisher: Elsevier BV
DOI: 10.1016/j.sbi.2019.05.015

Cholesterol Interaction Sites on the Transmembrane Domain of the Hedgehog Signal Transducer and Class F G Protein-Coupled Receptor Smoothened

Author(s): George Hedger, Heidi Koldsø, Matthieu Chavent, Christian Siebold, Rajat Rohatgi, Mark S.P. Sansom
Published in: Structure, Issue 27/3, 2019, Page(s) 549-559.e2, ISSN 0969-2126
Publisher: Cell Press
DOI: 10.1016/j.str.2018.11.003

Repulsive guidance molecules lock growth differentiation factor 5 in an inhibitory complex

Author(s): Tomas Malinauskas, Tina V. Peer, Benjamin Bishop, Thomas D. Mueller, Christian Siebold
Published in: Proceedings of the National Academy of Sciences, Issue 117/27, 2020, Page(s) 15620-15631, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2000561117

iASPP mediates p53 selectivity through a modular mechanism fine-tuning DNA recognition

Author(s): Shuo Chen, Jiale Wu, Shan Zhong, Yuntong Li, Ping Zhang, Jingyi Ma, Jingshan Ren, Yun Tan, Yunhao Wang, Kin Fai Au, Christian Siebold, Gareth L. Bond, Zhu Chen, Min Lu, E. Yvonne Jones, Xin Lu
Published in: Proceedings of the National Academy of Sciences, Issue 116/35, 2019, Page(s) 17470-17479, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1909393116

R-spondins engage heparan sulfate proteoglycans to potentiate WNT signaling

Author(s): Ramin Dubey, Peter van Kerkhof, Ingrid Jordens, Tomas Malinauskas, Ganesh V Pusapati, Joseph K McKenna, Dan Li, Jan E Carette, Mitchell Ho, Christian Siebold, Madelon Maurice, Andres M Lebensohn, Rajat Rohatgi
Published in: eLife, Issue 9, 2020, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.54469

Other (2)

Robin Hood: non-fitting, non-smoothing image detrending for bleaching correction

Author(s): Rory Nolan, Maro Iliopoulou, Christian Siebold, E. Yvonne Jones, Sergi Padilla-Parra
Published in: bioRxiv 667824, 2019
Publisher: Cold Spring Harbor bioRxiv
DOI: 10.1101/667824

Structure and Mechanism of Hedgehog Acyl Transferase

Author(s): Claire E. Coupland, Sebastian A. Andrei, T. Bertie Ansell, Loic Carrique, Pramod Kumar, Lea Sefer, Rebekka A Schwab, Eamon F.X. Byrne, Els Pardon, Jan Steyaert, Anthony I. Magee, Thomas. Lanyon-Hogg, Mark S. P. Sansom, Edward W. Tate, Christian Siebold
Published in: bioRxiv 451580, 2021
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.07.08.451580