Molecular analysis of the Hedgehog signal transduction complex in the primary cilium

Informacje na temat projektu

CilDyn

Identyfikator umowy o grant: 647278

DOI

10.3030/647278

Projekt został zamknięty

Data podpisania przez KE 16 Lipca 2015

Data rozpoczęcia 1 Sierpnia 2015

Data zakończenia 31 Stycznia 2021

Finansowanie w ramach

• EXCELLENT SCIENCE - European Research Council (ERC)

Koszt całkowity

€ 1 727 456,00

Wkład UE

€ 1 727 456,00

Koordynowany przez

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
United Kingdom

CORDIS oferuje możliwość skorzystania z odnośników do publicznie dostępnych publikacji i rezultatów projektów realizowanych w ramach programów ramowych HORYZONT.

Odnośniki do rezultatów i publikacji związanych z poszczególnymi projektami 7PR, a także odnośniki do niektórych konkretnych kategorii wyników, takich jak zbiory danych i oprogramowanie, są dynamicznie pobierane z systemu OpenAIRE .

Publikacje

Peer reviewed articles (22)

Cholesterol access in cellular membranes controls Hedgehog signaling

Autorzy: Arun Radhakrishnan, Rajat Rohatgi, Christian Siebold
Opublikowane w: Nature Chemical Biology, Numer 16/12, 2020, Strona(/y) 1303-1313, ISSN 1552-4450
Wydawca: Nature Publishing Group
DOI: 10.1038/s41589-020-00678-2

Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling

Autorzy: Ross A. Robinson, Samuel C. Griffiths, Lieke L. van de Haar, Tomas Malinauskas, Eljo Y. van Battum, Pavol Zelina, Rebekka A. Schwab, Dimple Karia, Lina Malinauskaite, Sara Brignani, Marleen H. van den Munkhof, Özge Düdükcü, Anna A. De Ruiter, Dianne M.A. Van den Heuvel, Benjamin Bishop, Jonathan Elegheert, A. Radu Aricescu, R. Jeroen Pasterkamp, Christian Siebold
Opublikowane w: Cell, Numer 184/8, 2021, Strona(/y) 2103-2120.e31, ISSN 0092-8674
Wydawca: Cell Press
DOI: 10.1016/j.cell.2021.02.045

Photochemical Probe Identification of a Small‐Molecule Inhibitor Binding Site in Hedgehog Acyltransferase (HHAT)**

Autorzy: Thomas Lanyon‐Hogg, Markus Ritzefeld, Leran Zhang, Sebastian A. Andrei, Balazs Pogranyi, Milon Mondal, Lea Sefer, Callum D. Johnston, Claire E. Coupland, Jake L. Greenfield, Joshua Newington, Matthew J. Fuchter, Anthony I. Magee, Christian Siebold, Edward W. Tate
Opublikowane w: Angewandte Chemie International Edition, Numer 60/24, 2021, Strona(/y) 13542-13547, ISSN 1433-7851
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202014457

RGMs: Structural Insights, Molecular Regulation, and Downstream Signaling

Autorzy: Christian Siebold, Toshihide Yamashita, Philippe P. Monnier, Bernhard K. Mueller, R. Jeroen Pasterkamp
Opublikowane w: Trends in Cell Biology, Numer 27/5, 2017, Strona(/y) 365-378, ISSN 0962-8924
Wydawca: Elsevier BV
DOI: 10.1016/j.tcb.2016.11.009

Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling

Autorzy: Giovanni Luchetti, Ria Sircar, Jennifer H Kong, Sigrid Nachtergaele, Andreas Sagner, Eamon FX Byrne, Douglas F Covey, Christian Siebold, Rajat Rohatgi
Opublikowane w: eLife, Numer 5, 2016, ISSN 2050-084X
Wydawca: eLife Sciences Publications
DOI: 10.7554/eLife.20304

A calcium-sensing receptor mutation causing hypocalcemia disrupts a transmembrane salt bridge to activate β-arrestin–biased signaling

Autorzy: Caroline M. Gorvin, Valerie N. Babinsky, Tomas Malinauskas, Peter H. Nissen, Anders J. Schou, Aylin C. Hanyaloglu, Christian Siebold, E. Yvonne Jones, Fadil M. Hannan, Rajesh V. Thakker
Opublikowane w: Science Signaling, Numer 11/518, 2018, Strona(/y) eaan3714, ISSN 1937-9145
Wydawca: American Association for the Advancement of Science
DOI: 10.1126/scisignal.aan3714

Multiple ligand binding sites regulate the Hedgehog signal transducer Smoothened in vertebrates

Autorzy: Eamon FX Byrne, Giovanni Luchetti, Rajat Rohatgi, Christian Siebold
Opublikowane w: Current Opinion in Cell Biology, Numer 51, 2018, Strona(/y) 81-88, ISSN 0955-0674
Wydawca: Elsevier BV
DOI: 10.1016/j.ceb.2017.10.004

Structural basis of Smoothened regulation by its extracellular domains

Autorzy: Eamon F. X. Byrne, Ria Sircar, Paul S. Miller, George Hedger, Giovanni Luchetti, Sigrid Nachtergaele, Mark D. Tully, Laurel Mydock-McGrane, Douglas F. Covey, Robert P. Rambo, Mark S. P. Sansom, Simon Newstead, Rajat Rohatgi, Christian Siebold
Opublikowane w: Nature, Numer 535/7613, 2016, Strona(/y) 517-522, ISSN 0028-0836
Wydawca: Nature Publishing Group
DOI: 10.1038/nature18934

Calibration-free <em>In Vitro</em> Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Autorzy: Rory Nolan, Luis A. Alvarez, Samuel C. Griffiths, Jonathan Elegheert, Christian Siebold, Sergi Padilla-Parra
Opublikowane w: Journal of Visualized Experiments, Numer 137, 2018, ISSN 1940-087X
Wydawca: MYJoVE Corporation
DOI: 10.3791/58157

Cilia-Associated Oxysterols Activate Smoothened

Autorzy: David R. Raleigh, Navdar Sever, Pervinder K. Choksi, Monika Abedin Sigg, Kelly M. Hines, Bonne M. Thompson, Daniel Elnatan, Priyadarshini Jaishankar, Paola Bisignano, Francesc R. Garcia-Gonzalo, Alexis Leigh Krup, Markus Eberl, Eamon F.X. Byrne, Christian Siebold, Sunny Y. Wong, Adam R. Renslo, Michael Grabe, Jeffrey G. McDonald, Libin Xu, Philip A. Beachy, Jeremy F. Reiter
Opublikowane w: Molecular Cell, Numer 72/2, 2018, Strona(/y) 316-327.e5, ISSN 1097-2765
Wydawca: Cell Press
DOI: 10.1016/j.molcel.2018.08.034

Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins

Autorzy: Jonathan Elegheert, Ester Behiels, Benjamin Bishop, Suzanne Scott, Rachel E. Woolley, Samuel C. Griffiths, Eamon F. X. Byrne, Veronica T. Chang, David I. Stuart, E. Yvonne Jones, Christian Siebold, A. Radu Aricescu
Opublikowane w: Nature Protocols, Numer 13/12, 2018, Strona(/y) 2991-3017, ISSN 1754-2189
Wydawca: Nature Publishing Group
DOI: 10.1038/s41596-018-0075-9

Calcium-sensing receptor residues with loss- and gain-of-function mutations are located in regions of conformational change and cause signalling bias

Autorzy: Caroline M Gorvin, Morten Frost, Tomas Malinauskas, Treena Cranston, Hannah Boon, Christian Siebold, E Yvonne Jones, Fadil M Hannan, Rajesh V Thakker
Opublikowane w: Human Molecular Genetics, Numer 27/21, 2018, Strona(/y) 3720-3733, ISSN 0964-6906
Wydawca: Oxford University Press
DOI: 10.1093/hmg/ddy263

The morphogen Sonic hedgehog inhibits its receptor Patched by a pincer grasp mechanism

Autorzy: Amalie F. Rudolf, Maia Kinnebrew, Christiane Kowatsch, T. Bertie Ansell, Kamel El Omari, Benjamin Bishop, Els Pardon, Rebekka A. Schwab, Tomas Malinauskas, Mingxing Qian, Ramona Duman, Douglas F. Covey, Jan Steyaert, Armin Wagner, Mark S. P. Sansom, Rajat Rohatgi, Christian Siebold
Opublikowane w: Nature Chemical Biology, Numer 15/10, 2019, Strona(/y) 975-982, ISSN 1552-4450
Wydawca: Nature Publishing Group
DOI: 10.1038/s41589-019-0370-y

Cholesterol accessibility at the ciliary membrane controls hedgehog signaling

Autorzy: Maia Kinnebrew, Ellen J Iverson, Bhaven B Patel, Ganesh V Pusapati, Jennifer H Kong, Kristen A Johnson, Giovanni Luchetti, Kaitlyn M Eckert, Jeffrey G McDonald, Douglas F Covey, Christian Siebold, Arun Radhakrishnan, Rajat Rohatgi
Opublikowane w: eLife, Numer 8, 2019, ISSN 2050-084X
Wydawca: eLife Sciences Publications
DOI: 10.7554/elife.50051

Biochemical mechanisms of vertebrate hedgehog signaling

Autorzy: Jennifer H. Kong, Christian Siebold, Rajat Rohatgi
Opublikowane w: Development, Numer 146/10, 2019, Strona(/y) dev166892, ISSN 0950-1991
Wydawca: The Company of Biologists Ltd.
DOI: 10.1242/dev.166892

Acylation-coupled lipophilic induction of polarisation (Acyl-cLIP): a universal assay for lipid transferase and hydrolase enzymes

Autorzy: Thomas Lanyon-Hogg, Markus Ritzefeld, Lea Sefer, Jasmine K. Bickel, Amalie F. Rudolf, Nattawadee Panyain, Ganka Bineva-Todd, Cory A. Ocasio, Nicola O'Reilly, Christian Siebold, Anthony I. Magee, Edward W. Tate
Opublikowane w: Chemical Science, Numer 10/39, 2019, Strona(/y) 8995-9000, ISSN 2041-6520
Wydawca: Royal Society of Chemistry
DOI: 10.1039/c9sc01785b

Diversity of oligomerization in Drosophila semaphorins suggests a mechanism of functional fine-tuning

Autorzy: Daniel Rozbesky, Ross A. Robinson, Vitul Jain, Max Renner, Tomas Malinauskas, Karl Harlos, Christian Siebold, E. Yvonne Jones
Opublikowane w: Nature Communications, Numer 10/1, 2019, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-019-11683-y

Structures of vertebrate Patched and Smoothened reveal intimate links between cholesterol and Hedgehog signalling

Autorzy: Christiane Kowatsch, Rachel E Woolley, Maia Kinnebrew, Rajat Rohatgi, Christian Siebold
Opublikowane w: Current Opinion in Structural Biology, Numer 57, 2019, Strona(/y) 204-214, ISSN 0959-440X
Wydawca: Elsevier BV
DOI: 10.1016/j.sbi.2019.05.015

Cholesterol Interaction Sites on the Transmembrane Domain of the Hedgehog Signal Transducer and Class F G Protein-Coupled Receptor Smoothened

Autorzy: George Hedger, Heidi Koldsø, Matthieu Chavent, Christian Siebold, Rajat Rohatgi, Mark S.P. Sansom
Opublikowane w: Structure, Numer 27/3, 2019, Strona(/y) 549-559.e2, ISSN 0969-2126
Wydawca: Cell Press
DOI: 10.1016/j.str.2018.11.003

Repulsive guidance molecules lock growth differentiation factor 5 in an inhibitory complex

Autorzy: Tomas Malinauskas, Tina V. Peer, Benjamin Bishop, Thomas D. Mueller, Christian Siebold
Opublikowane w: Proceedings of the National Academy of Sciences, Numer 117/27, 2020, Strona(/y) 15620-15631, ISSN 0027-8424
Wydawca: National Academy of Sciences
DOI: 10.1073/pnas.2000561117

iASPP mediates p53 selectivity through a modular mechanism fine-tuning DNA recognition

Autorzy: Shuo Chen, Jiale Wu, Shan Zhong, Yuntong Li, Ping Zhang, Jingyi Ma, Jingshan Ren, Yun Tan, Yunhao Wang, Kin Fai Au, Christian Siebold, Gareth L. Bond, Zhu Chen, Min Lu, E. Yvonne Jones, Xin Lu
Opublikowane w: Proceedings of the National Academy of Sciences, Numer 116/35, 2019, Strona(/y) 17470-17479, ISSN 0027-8424
Wydawca: National Academy of Sciences
DOI: 10.1073/pnas.1909393116

R-spondins engage heparan sulfate proteoglycans to potentiate WNT signaling

Autorzy: Ramin Dubey, Peter van Kerkhof, Ingrid Jordens, Tomas Malinauskas, Ganesh V Pusapati, Joseph K McKenna, Dan Li, Jan E Carette, Mitchell Ho, Christian Siebold, Madelon Maurice, Andres M Lebensohn, Rajat Rohatgi
Opublikowane w: eLife, Numer 9, 2020, ISSN 2050-084X
Wydawca: eLife Sciences Publications
DOI: 10.7554/elife.54469

Other (2)

Robin Hood: non-fitting, non-smoothing image detrending for bleaching correction

Autorzy: Rory Nolan, Maro Iliopoulou, Christian Siebold, E. Yvonne Jones, Sergi Padilla-Parra
Opublikowane w: bioRxiv 667824, 2019
Wydawca: Cold Spring Harbor bioRxiv
DOI: 10.1101/667824

Structure and Mechanism of Hedgehog Acyl Transferase

Autorzy: Claire E. Coupland, Sebastian A. Andrei, T. Bertie Ansell, Loic Carrique, Pramod Kumar, Lea Sefer, Rebekka A Schwab, Eamon F.X. Byrne, Els Pardon, Jan Steyaert, Anthony I. Magee, Thomas. Lanyon-Hogg, Mark S. P. Sansom, Edward W. Tate, Christian Siebold
Opublikowane w: bioRxiv 451580, 2021
Wydawca: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.07.08.451580