Molecular analysis of the Hedgehog signal transduction complex in the primary cilium

Informazioni relative al progetto

CilDyn

ID dell’accordo di sovvenzione: 647278

DOI

10.3030/647278

Progetto chiuso

Data della firma CE 16 Luglio 2015

Data di avvio 1 Agosto 2015

Data di completamento 31 Gennaio 2021

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 1 727 456,00

Contributo UE

€ 1 727 456,00

Coordinato da

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
United Kingdom

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (22)

Cholesterol access in cellular membranes controls Hedgehog signaling

Autori: Arun Radhakrishnan, Rajat Rohatgi, Christian Siebold
Pubblicato in: Nature Chemical Biology, Numero 16/12, 2020, Pagina/e 1303-1313, ISSN 1552-4450
Editore: Nature Publishing Group
DOI: 10.1038/s41589-020-00678-2

Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling

Autori: Ross A. Robinson, Samuel C. Griffiths, Lieke L. van de Haar, Tomas Malinauskas, Eljo Y. van Battum, Pavol Zelina, Rebekka A. Schwab, Dimple Karia, Lina Malinauskaite, Sara Brignani, Marleen H. van den Munkhof, Özge Düdükcü, Anna A. De Ruiter, Dianne M.A. Van den Heuvel, Benjamin Bishop, Jonathan Elegheert, A. Radu Aricescu, R. Jeroen Pasterkamp, Christian Siebold
Pubblicato in: Cell, Numero 184/8, 2021, Pagina/e 2103-2120.e31, ISSN 0092-8674
Editore: Cell Press
DOI: 10.1016/j.cell.2021.02.045

Photochemical Probe Identification of a Small‐Molecule Inhibitor Binding Site in Hedgehog Acyltransferase (HHAT)**

Autori: Thomas Lanyon‐Hogg, Markus Ritzefeld, Leran Zhang, Sebastian A. Andrei, Balazs Pogranyi, Milon Mondal, Lea Sefer, Callum D. Johnston, Claire E. Coupland, Jake L. Greenfield, Joshua Newington, Matthew J. Fuchter, Anthony I. Magee, Christian Siebold, Edward W. Tate
Pubblicato in: Angewandte Chemie International Edition, Numero 60/24, 2021, Pagina/e 13542-13547, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202014457

RGMs: Structural Insights, Molecular Regulation, and Downstream Signaling

Autori: Christian Siebold, Toshihide Yamashita, Philippe P. Monnier, Bernhard K. Mueller, R. Jeroen Pasterkamp
Pubblicato in: Trends in Cell Biology, Numero 27/5, 2017, Pagina/e 365-378, ISSN 0962-8924
Editore: Elsevier BV
DOI: 10.1016/j.tcb.2016.11.009

Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling

Autori: Giovanni Luchetti, Ria Sircar, Jennifer H Kong, Sigrid Nachtergaele, Andreas Sagner, Eamon FX Byrne, Douglas F Covey, Christian Siebold, Rajat Rohatgi
Pubblicato in: eLife, Numero 5, 2016, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/eLife.20304

A calcium-sensing receptor mutation causing hypocalcemia disrupts a transmembrane salt bridge to activate β-arrestin–biased signaling

Autori: Caroline M. Gorvin, Valerie N. Babinsky, Tomas Malinauskas, Peter H. Nissen, Anders J. Schou, Aylin C. Hanyaloglu, Christian Siebold, E. Yvonne Jones, Fadil M. Hannan, Rajesh V. Thakker
Pubblicato in: Science Signaling, Numero 11/518, 2018, Pagina/e eaan3714, ISSN 1937-9145
Editore: American Association for the Advancement of Science
DOI: 10.1126/scisignal.aan3714

Multiple ligand binding sites regulate the Hedgehog signal transducer Smoothened in vertebrates

Autori: Eamon FX Byrne, Giovanni Luchetti, Rajat Rohatgi, Christian Siebold
Pubblicato in: Current Opinion in Cell Biology, Numero 51, 2018, Pagina/e 81-88, ISSN 0955-0674
Editore: Elsevier BV
DOI: 10.1016/j.ceb.2017.10.004

Structural basis of Smoothened regulation by its extracellular domains

Autori: Eamon F. X. Byrne, Ria Sircar, Paul S. Miller, George Hedger, Giovanni Luchetti, Sigrid Nachtergaele, Mark D. Tully, Laurel Mydock-McGrane, Douglas F. Covey, Robert P. Rambo, Mark S. P. Sansom, Simon Newstead, Rajat Rohatgi, Christian Siebold
Pubblicato in: Nature, Numero 535/7613, 2016, Pagina/e 517-522, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/nature18934

Calibration-free <em>In Vitro</em> Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Autori: Rory Nolan, Luis A. Alvarez, Samuel C. Griffiths, Jonathan Elegheert, Christian Siebold, Sergi Padilla-Parra
Pubblicato in: Journal of Visualized Experiments, Numero 137, 2018, ISSN 1940-087X
Editore: MYJoVE Corporation
DOI: 10.3791/58157

Cilia-Associated Oxysterols Activate Smoothened

Autori: David R. Raleigh, Navdar Sever, Pervinder K. Choksi, Monika Abedin Sigg, Kelly M. Hines, Bonne M. Thompson, Daniel Elnatan, Priyadarshini Jaishankar, Paola Bisignano, Francesc R. Garcia-Gonzalo, Alexis Leigh Krup, Markus Eberl, Eamon F.X. Byrne, Christian Siebold, Sunny Y. Wong, Adam R. Renslo, Michael Grabe, Jeffrey G. McDonald, Libin Xu, Philip A. Beachy, Jeremy F. Reiter
Pubblicato in: Molecular Cell, Numero 72/2, 2018, Pagina/e 316-327.e5, ISSN 1097-2765
Editore: Cell Press
DOI: 10.1016/j.molcel.2018.08.034

Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins

Autori: Jonathan Elegheert, Ester Behiels, Benjamin Bishop, Suzanne Scott, Rachel E. Woolley, Samuel C. Griffiths, Eamon F. X. Byrne, Veronica T. Chang, David I. Stuart, E. Yvonne Jones, Christian Siebold, A. Radu Aricescu
Pubblicato in: Nature Protocols, Numero 13/12, 2018, Pagina/e 2991-3017, ISSN 1754-2189
Editore: Nature Publishing Group
DOI: 10.1038/s41596-018-0075-9

Calcium-sensing receptor residues with loss- and gain-of-function mutations are located in regions of conformational change and cause signalling bias

Autori: Caroline M Gorvin, Morten Frost, Tomas Malinauskas, Treena Cranston, Hannah Boon, Christian Siebold, E Yvonne Jones, Fadil M Hannan, Rajesh V Thakker
Pubblicato in: Human Molecular Genetics, Numero 27/21, 2018, Pagina/e 3720-3733, ISSN 0964-6906
Editore: Oxford University Press
DOI: 10.1093/hmg/ddy263

The morphogen Sonic hedgehog inhibits its receptor Patched by a pincer grasp mechanism

Autori: Amalie F. Rudolf, Maia Kinnebrew, Christiane Kowatsch, T. Bertie Ansell, Kamel El Omari, Benjamin Bishop, Els Pardon, Rebekka A. Schwab, Tomas Malinauskas, Mingxing Qian, Ramona Duman, Douglas F. Covey, Jan Steyaert, Armin Wagner, Mark S. P. Sansom, Rajat Rohatgi, Christian Siebold
Pubblicato in: Nature Chemical Biology, Numero 15/10, 2019, Pagina/e 975-982, ISSN 1552-4450
Editore: Nature Publishing Group
DOI: 10.1038/s41589-019-0370-y

Cholesterol accessibility at the ciliary membrane controls hedgehog signaling

Autori: Maia Kinnebrew, Ellen J Iverson, Bhaven B Patel, Ganesh V Pusapati, Jennifer H Kong, Kristen A Johnson, Giovanni Luchetti, Kaitlyn M Eckert, Jeffrey G McDonald, Douglas F Covey, Christian Siebold, Arun Radhakrishnan, Rajat Rohatgi
Pubblicato in: eLife, Numero 8, 2019, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.50051

Biochemical mechanisms of vertebrate hedgehog signaling

Autori: Jennifer H. Kong, Christian Siebold, Rajat Rohatgi
Pubblicato in: Development, Numero 146/10, 2019, Pagina/e dev166892, ISSN 0950-1991
Editore: The Company of Biologists Ltd.
DOI: 10.1242/dev.166892

Acylation-coupled lipophilic induction of polarisation (Acyl-cLIP): a universal assay for lipid transferase and hydrolase enzymes

Autori: Thomas Lanyon-Hogg, Markus Ritzefeld, Lea Sefer, Jasmine K. Bickel, Amalie F. Rudolf, Nattawadee Panyain, Ganka Bineva-Todd, Cory A. Ocasio, Nicola O'Reilly, Christian Siebold, Anthony I. Magee, Edward W. Tate
Pubblicato in: Chemical Science, Numero 10/39, 2019, Pagina/e 8995-9000, ISSN 2041-6520
Editore: Royal Society of Chemistry
DOI: 10.1039/c9sc01785b

Diversity of oligomerization in Drosophila semaphorins suggests a mechanism of functional fine-tuning

Autori: Daniel Rozbesky, Ross A. Robinson, Vitul Jain, Max Renner, Tomas Malinauskas, Karl Harlos, Christian Siebold, E. Yvonne Jones
Pubblicato in: Nature Communications, Numero 10/1, 2019, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-019-11683-y

Structures of vertebrate Patched and Smoothened reveal intimate links between cholesterol and Hedgehog signalling

Autori: Christiane Kowatsch, Rachel E Woolley, Maia Kinnebrew, Rajat Rohatgi, Christian Siebold
Pubblicato in: Current Opinion in Structural Biology, Numero 57, 2019, Pagina/e 204-214, ISSN 0959-440X
Editore: Elsevier BV
DOI: 10.1016/j.sbi.2019.05.015

Cholesterol Interaction Sites on the Transmembrane Domain of the Hedgehog Signal Transducer and Class F G Protein-Coupled Receptor Smoothened

Autori: George Hedger, Heidi Koldsø, Matthieu Chavent, Christian Siebold, Rajat Rohatgi, Mark S.P. Sansom
Pubblicato in: Structure, Numero 27/3, 2019, Pagina/e 549-559.e2, ISSN 0969-2126
Editore: Cell Press
DOI: 10.1016/j.str.2018.11.003

Repulsive guidance molecules lock growth differentiation factor 5 in an inhibitory complex

Autori: Tomas Malinauskas, Tina V. Peer, Benjamin Bishop, Thomas D. Mueller, Christian Siebold
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 117/27, 2020, Pagina/e 15620-15631, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.2000561117

iASPP mediates p53 selectivity through a modular mechanism fine-tuning DNA recognition

Autori: Shuo Chen, Jiale Wu, Shan Zhong, Yuntong Li, Ping Zhang, Jingyi Ma, Jingshan Ren, Yun Tan, Yunhao Wang, Kin Fai Au, Christian Siebold, Gareth L. Bond, Zhu Chen, Min Lu, E. Yvonne Jones, Xin Lu
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 116/35, 2019, Pagina/e 17470-17479, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.1909393116

R-spondins engage heparan sulfate proteoglycans to potentiate WNT signaling

Autori: Ramin Dubey, Peter van Kerkhof, Ingrid Jordens, Tomas Malinauskas, Ganesh V Pusapati, Joseph K McKenna, Dan Li, Jan E Carette, Mitchell Ho, Christian Siebold, Madelon Maurice, Andres M Lebensohn, Rajat Rohatgi
Pubblicato in: eLife, Numero 9, 2020, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.54469

Other (2)

Robin Hood: non-fitting, non-smoothing image detrending for bleaching correction

Autori: Rory Nolan, Maro Iliopoulou, Christian Siebold, E. Yvonne Jones, Sergi Padilla-Parra
Pubblicato in: bioRxiv 667824, 2019
Editore: Cold Spring Harbor bioRxiv
DOI: 10.1101/667824

Structure and Mechanism of Hedgehog Acyl Transferase

Autori: Claire E. Coupland, Sebastian A. Andrei, T. Bertie Ansell, Loic Carrique, Pramod Kumar, Lea Sefer, Rebekka A Schwab, Eamon F.X. Byrne, Els Pardon, Jan Steyaert, Anthony I. Magee, Thomas. Lanyon-Hogg, Mark S. P. Sansom, Edward W. Tate, Christian Siebold
Pubblicato in: bioRxiv 451580, 2021
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.07.08.451580