Molecular analysis of the Hedgehog signal transduction complex in the primary cilium

Información del proyecto

CilDyn

Identificador del acuerdo de subvención: 647278

DOI

10.3030/647278

Proyecto cerrado

Fecha de la firma de la CE 16 Julio 2015

Fecha de inicio 1 Agosto 2015

Fecha de finalización 31 Enero 2021

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 1 727 456,00

Aportación de la UE

€ 1 727 456,00

Coordinado por

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
United Kingdom

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Publicaciones

Peer reviewed articles (22)

Cholesterol access in cellular membranes controls Hedgehog signaling

Autores: Arun Radhakrishnan, Rajat Rohatgi, Christian Siebold
Publicado en: Nature Chemical Biology, Edición 16/12, 2020, Página(s) 1303-1313, ISSN 1552-4450
Editor: Nature Publishing Group
DOI: 10.1038/s41589-020-00678-2

Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling

Autores: Ross A. Robinson, Samuel C. Griffiths, Lieke L. van de Haar, Tomas Malinauskas, Eljo Y. van Battum, Pavol Zelina, Rebekka A. Schwab, Dimple Karia, Lina Malinauskaite, Sara Brignani, Marleen H. van den Munkhof, Özge Düdükcü, Anna A. De Ruiter, Dianne M.A. Van den Heuvel, Benjamin Bishop, Jonathan Elegheert, A. Radu Aricescu, R. Jeroen Pasterkamp, Christian Siebold
Publicado en: Cell, Edición 184/8, 2021, Página(s) 2103-2120.e31, ISSN 0092-8674
Editor: Cell Press
DOI: 10.1016/j.cell.2021.02.045

Photochemical Probe Identification of a Small‐Molecule Inhibitor Binding Site in Hedgehog Acyltransferase (HHAT)**

Autores: Thomas Lanyon‐Hogg, Markus Ritzefeld, Leran Zhang, Sebastian A. Andrei, Balazs Pogranyi, Milon Mondal, Lea Sefer, Callum D. Johnston, Claire E. Coupland, Jake L. Greenfield, Joshua Newington, Matthew J. Fuchter, Anthony I. Magee, Christian Siebold, Edward W. Tate
Publicado en: Angewandte Chemie International Edition, Edición 60/24, 2021, Página(s) 13542-13547, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202014457

RGMs: Structural Insights, Molecular Regulation, and Downstream Signaling

Autores: Christian Siebold, Toshihide Yamashita, Philippe P. Monnier, Bernhard K. Mueller, R. Jeroen Pasterkamp
Publicado en: Trends in Cell Biology, Edición 27/5, 2017, Página(s) 365-378, ISSN 0962-8924
Editor: Elsevier BV
DOI: 10.1016/j.tcb.2016.11.009

Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling

Autores: Giovanni Luchetti, Ria Sircar, Jennifer H Kong, Sigrid Nachtergaele, Andreas Sagner, Eamon FX Byrne, Douglas F Covey, Christian Siebold, Rajat Rohatgi
Publicado en: eLife, Edición 5, 2016, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.7554/eLife.20304

A calcium-sensing receptor mutation causing hypocalcemia disrupts a transmembrane salt bridge to activate β-arrestin–biased signaling

Autores: Caroline M. Gorvin, Valerie N. Babinsky, Tomas Malinauskas, Peter H. Nissen, Anders J. Schou, Aylin C. Hanyaloglu, Christian Siebold, E. Yvonne Jones, Fadil M. Hannan, Rajesh V. Thakker
Publicado en: Science Signaling, Edición 11/518, 2018, Página(s) eaan3714, ISSN 1937-9145
Editor: American Association for the Advancement of Science
DOI: 10.1126/scisignal.aan3714

Multiple ligand binding sites regulate the Hedgehog signal transducer Smoothened in vertebrates

Autores: Eamon FX Byrne, Giovanni Luchetti, Rajat Rohatgi, Christian Siebold
Publicado en: Current Opinion in Cell Biology, Edición 51, 2018, Página(s) 81-88, ISSN 0955-0674
Editor: Elsevier BV
DOI: 10.1016/j.ceb.2017.10.004

Structural basis of Smoothened regulation by its extracellular domains

Autores: Eamon F. X. Byrne, Ria Sircar, Paul S. Miller, George Hedger, Giovanni Luchetti, Sigrid Nachtergaele, Mark D. Tully, Laurel Mydock-McGrane, Douglas F. Covey, Robert P. Rambo, Mark S. P. Sansom, Simon Newstead, Rajat Rohatgi, Christian Siebold
Publicado en: Nature, Edición 535/7613, 2016, Página(s) 517-522, ISSN 0028-0836
Editor: Nature Publishing Group
DOI: 10.1038/nature18934

Calibration-free <em>In Vitro</em> Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Autores: Rory Nolan, Luis A. Alvarez, Samuel C. Griffiths, Jonathan Elegheert, Christian Siebold, Sergi Padilla-Parra
Publicado en: Journal of Visualized Experiments, Edición 137, 2018, ISSN 1940-087X
Editor: MYJoVE Corporation
DOI: 10.3791/58157

Cilia-Associated Oxysterols Activate Smoothened

Autores: David R. Raleigh, Navdar Sever, Pervinder K. Choksi, Monika Abedin Sigg, Kelly M. Hines, Bonne M. Thompson, Daniel Elnatan, Priyadarshini Jaishankar, Paola Bisignano, Francesc R. Garcia-Gonzalo, Alexis Leigh Krup, Markus Eberl, Eamon F.X. Byrne, Christian Siebold, Sunny Y. Wong, Adam R. Renslo, Michael Grabe, Jeffrey G. McDonald, Libin Xu, Philip A. Beachy, Jeremy F. Reiter
Publicado en: Molecular Cell, Edición 72/2, 2018, Página(s) 316-327.e5, ISSN 1097-2765
Editor: Cell Press
DOI: 10.1016/j.molcel.2018.08.034

Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins

Autores: Jonathan Elegheert, Ester Behiels, Benjamin Bishop, Suzanne Scott, Rachel E. Woolley, Samuel C. Griffiths, Eamon F. X. Byrne, Veronica T. Chang, David I. Stuart, E. Yvonne Jones, Christian Siebold, A. Radu Aricescu
Publicado en: Nature Protocols, Edición 13/12, 2018, Página(s) 2991-3017, ISSN 1754-2189
Editor: Nature Publishing Group
DOI: 10.1038/s41596-018-0075-9

Calcium-sensing receptor residues with loss- and gain-of-function mutations are located in regions of conformational change and cause signalling bias

Autores: Caroline M Gorvin, Morten Frost, Tomas Malinauskas, Treena Cranston, Hannah Boon, Christian Siebold, E Yvonne Jones, Fadil M Hannan, Rajesh V Thakker
Publicado en: Human Molecular Genetics, Edición 27/21, 2018, Página(s) 3720-3733, ISSN 0964-6906
Editor: Oxford University Press
DOI: 10.1093/hmg/ddy263

The morphogen Sonic hedgehog inhibits its receptor Patched by a pincer grasp mechanism

Autores: Amalie F. Rudolf, Maia Kinnebrew, Christiane Kowatsch, T. Bertie Ansell, Kamel El Omari, Benjamin Bishop, Els Pardon, Rebekka A. Schwab, Tomas Malinauskas, Mingxing Qian, Ramona Duman, Douglas F. Covey, Jan Steyaert, Armin Wagner, Mark S. P. Sansom, Rajat Rohatgi, Christian Siebold
Publicado en: Nature Chemical Biology, Edición 15/10, 2019, Página(s) 975-982, ISSN 1552-4450
Editor: Nature Publishing Group
DOI: 10.1038/s41589-019-0370-y

Cholesterol accessibility at the ciliary membrane controls hedgehog signaling

Autores: Maia Kinnebrew, Ellen J Iverson, Bhaven B Patel, Ganesh V Pusapati, Jennifer H Kong, Kristen A Johnson, Giovanni Luchetti, Kaitlyn M Eckert, Jeffrey G McDonald, Douglas F Covey, Christian Siebold, Arun Radhakrishnan, Rajat Rohatgi
Publicado en: eLife, Edición 8, 2019, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.7554/elife.50051

Biochemical mechanisms of vertebrate hedgehog signaling

Autores: Jennifer H. Kong, Christian Siebold, Rajat Rohatgi
Publicado en: Development, Edición 146/10, 2019, Página(s) dev166892, ISSN 0950-1991
Editor: The Company of Biologists Ltd.
DOI: 10.1242/dev.166892

Acylation-coupled lipophilic induction of polarisation (Acyl-cLIP): a universal assay for lipid transferase and hydrolase enzymes

Autores: Thomas Lanyon-Hogg, Markus Ritzefeld, Lea Sefer, Jasmine K. Bickel, Amalie F. Rudolf, Nattawadee Panyain, Ganka Bineva-Todd, Cory A. Ocasio, Nicola O'Reilly, Christian Siebold, Anthony I. Magee, Edward W. Tate
Publicado en: Chemical Science, Edición 10/39, 2019, Página(s) 8995-9000, ISSN 2041-6520
Editor: Royal Society of Chemistry
DOI: 10.1039/c9sc01785b

Diversity of oligomerization in Drosophila semaphorins suggests a mechanism of functional fine-tuning

Autores: Daniel Rozbesky, Ross A. Robinson, Vitul Jain, Max Renner, Tomas Malinauskas, Karl Harlos, Christian Siebold, E. Yvonne Jones
Publicado en: Nature Communications, Edición 10/1, 2019, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-019-11683-y

Structures of vertebrate Patched and Smoothened reveal intimate links between cholesterol and Hedgehog signalling

Autores: Christiane Kowatsch, Rachel E Woolley, Maia Kinnebrew, Rajat Rohatgi, Christian Siebold
Publicado en: Current Opinion in Structural Biology, Edición 57, 2019, Página(s) 204-214, ISSN 0959-440X
Editor: Elsevier BV
DOI: 10.1016/j.sbi.2019.05.015

Cholesterol Interaction Sites on the Transmembrane Domain of the Hedgehog Signal Transducer and Class F G Protein-Coupled Receptor Smoothened

Autores: George Hedger, Heidi Koldsø, Matthieu Chavent, Christian Siebold, Rajat Rohatgi, Mark S.P. Sansom
Publicado en: Structure, Edición 27/3, 2019, Página(s) 549-559.e2, ISSN 0969-2126
Editor: Cell Press
DOI: 10.1016/j.str.2018.11.003

Repulsive guidance molecules lock growth differentiation factor 5 in an inhibitory complex

Autores: Tomas Malinauskas, Tina V. Peer, Benjamin Bishop, Thomas D. Mueller, Christian Siebold
Publicado en: Proceedings of the National Academy of Sciences, Edición 117/27, 2020, Página(s) 15620-15631, ISSN 0027-8424
Editor: National Academy of Sciences
DOI: 10.1073/pnas.2000561117

iASPP mediates p53 selectivity through a modular mechanism fine-tuning DNA recognition

Autores: Shuo Chen, Jiale Wu, Shan Zhong, Yuntong Li, Ping Zhang, Jingyi Ma, Jingshan Ren, Yun Tan, Yunhao Wang, Kin Fai Au, Christian Siebold, Gareth L. Bond, Zhu Chen, Min Lu, E. Yvonne Jones, Xin Lu
Publicado en: Proceedings of the National Academy of Sciences, Edición 116/35, 2019, Página(s) 17470-17479, ISSN 0027-8424
Editor: National Academy of Sciences
DOI: 10.1073/pnas.1909393116

R-spondins engage heparan sulfate proteoglycans to potentiate WNT signaling

Autores: Ramin Dubey, Peter van Kerkhof, Ingrid Jordens, Tomas Malinauskas, Ganesh V Pusapati, Joseph K McKenna, Dan Li, Jan E Carette, Mitchell Ho, Christian Siebold, Madelon Maurice, Andres M Lebensohn, Rajat Rohatgi
Publicado en: eLife, Edición 9, 2020, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.7554/elife.54469

Other (2)

Robin Hood: non-fitting, non-smoothing image detrending for bleaching correction

Autores: Rory Nolan, Maro Iliopoulou, Christian Siebold, E. Yvonne Jones, Sergi Padilla-Parra
Publicado en: bioRxiv 667824, 2019
Editor: Cold Spring Harbor bioRxiv
DOI: 10.1101/667824

Structure and Mechanism of Hedgehog Acyl Transferase

Autores: Claire E. Coupland, Sebastian A. Andrei, T. Bertie Ansell, Loic Carrique, Pramod Kumar, Lea Sefer, Rebekka A Schwab, Eamon F.X. Byrne, Els Pardon, Jan Steyaert, Anthony I. Magee, Thomas. Lanyon-Hogg, Mark S. P. Sansom, Edward W. Tate, Christian Siebold
Publicado en: bioRxiv 451580, 2021
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.07.08.451580