Elucidating natural bilayer lipid environments

Project Information

ENABLE

Grant agreement ID: 695511

DOI 10.3030/695511

Project closed

EC signature date 17 May 2016

Start date 1 June 2016

End date 31 May 2021

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 481 744,00

EU contribution € 2 481 744,00

Coordinated by

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
United Kingdom

Publications

Peer reviewed articles (22)

N-glycan microheterogeneity regulates interactions of plasma proteins

Author(s): Di Wu, Weston B. Struwe, David J. Harvey, Michael A. J. Ferguson, Carol V. Robinson
Published in: Proceedings of the National Academy of Sciences, Issue 115/35, 2018, Page(s) 8763-8768, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1807439115

Allosteric Inhibition of the SARS‐CoV‐2 Main Protease: Insights from Mass Spectrometry Based Assays**

Author(s): Tarick J. El‐Baba, Corinne A. Lutomski, Anastassia L. Kantsadi, Tika R. Malla, Tobias John, Victor Mikhailov, Jani R. Bolla, Christopher J. Schofield, Nicole Zitzmann, Ioannis Vakonakis, Carol V. Robinson
Published in: Angewandte Chemie International Edition, Issue 59/52, 2020, Page(s) 23544-23548, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202010316

Dynamics of an LPS translocon induced by substrate and an antimicrobial peptide

Author(s): Francesco Fiorentino, Joshua B. Sauer, Xingyu Qiu, Robin A. Corey, C. Keith Cassidy, Benjamin Mynors-Wallis, Shahid Mehmood, Jani R. Bolla, Phillip J. Stansfeld, Carol V. Robinson
Published in: Nature Chemical Biology, Issue 17/2, 2021, Page(s) 187-195, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-020-00694-2

Structure of native lens connexin 46/50 intercellular channels by cryo-EM

Author(s): Janette B. Myers, Bassam G. Haddad, Susan E. O’Neill, Dror S. Chorev, Craig C. Yoshioka, Carol V. Robinson, Daniel M. Zuckerman, Steve L. Reichow
Published in: Nature, Issue 564/7736, 2018, Page(s) 372-377, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-018-0786-7

The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes

Author(s): Dror S. Chorev, Haiping Tang, Sarah L. Rouse, Jani Reddy Bolla, Andriko von Kügelgen, Lindsay A. Baker, Di Wu, Joseph Gault, Kay Grünewald, Tanmay A. M. Bharat, Stephen J. Matthews, Carol V. Robinson
Published in: Nature Protocols, Issue 15/5, 2020, Page(s) 1690-1706, ISSN 1754-2189
Publisher: Nature Publishing Group
DOI: 10.1038/s41596-020-0303-y

Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins

Author(s): Joseph Gault, Idlir Liko, Michael Landreh, Denis Shutin, Jani Reddy Bolla, Damien Jefferies, Mark Agasid, Hsin-Yung Yen, Marcus J. G. W. Ladds, David P. Lane, Syma Khalid, Christopher Mullen, Philip M. Remes, Romain Huguet, Graeme McAlister, Michael Goodwin, Rosa Viner, John E.P. Syka, Carol V. Robinson
Published in: Nature Methods, Issue 17/5, 2020, Page(s) 505-508, ISSN 1548-7091
Publisher: Nature Publishing Group
DOI: 10.1038/s41592-020-0821-0

Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors

Author(s): Leonhard H. Urner, Idlir Liko, Hsin-Yung Yen, Kin-Kuan Hoi, Jani Reddy Bolla, Joseph Gault, Fernando Gonçalves Almeida, Marc-Philip Schweder, Denis Shutin, Svenja Ehrmann, Rainer Haag, Carol V. Robinson, Kevin Pagel
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-14424-8

In Situ Structure of an Intact Lipopolysaccharide-Bound Bacterial Surface Layer

Author(s): Andriko von Kügelgen, Haiping Tang, Gail G. Hardy, Danguole Kureisaite-Ciziene, Yves V. Brun, Phillip J. Stansfeld, Carol V. Robinson, Tanmay A.M. Bharat
Published in: Cell, Issue 180/2, 2020, Page(s) 348-358.e15, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2019.12.006

Phospho‐regulation, nucleotide binding and ion access control in potassium‐chloride cotransporters

Author(s): Gamma Chi, Rebecca Ebenhoch, Henry Man, Haiping Tang, Laurence E Tremblay, Gabriella Reggiano, Xingyu Qiu, Tina Bohstedt, Idlir Liko, Fernando G Almeida, Alexandre P Garneau, Dong Wang, Gavin McKinley, Christophe P Moreau, Kiran D Bountra, Patrizia Abrusci, Shubhashish M M Mukhopadhyay, Alejandra Fernandez‐Cid, Samira Slimani, Julie L Lavoie, Nicola A Burgess‐Brown, Ben Tehan, Frank DiMaio, Al
Published in: The EMBO Journal, 2021, ISSN 0261-4189
Publisher: Nature Publishing Group
DOI: 10.15252/embj.2020107294

Dendritic Oligoglycerol Regioisomer Mixtures and Their Utility for Membrane Protein Research

Author(s): Leonhard H. Urner, Katharina Goltsche, Marleen Selent, Idlir Liko, Marc‐Philip Schweder, Carol V. Robinson, Kevin Pagel, Rainer Haag
Published in: Chemistry – A European Journal, Issue 27/7, 2021, Page(s) 2537-2542, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202003991

A new azobenzene-based design strategy for detergents in membrane protein research

Author(s): Leonhard H. Urner, Maiko Schulze, Yasmine B. Maier, Waldemar Hoffmann, Stephan Warnke, Idlir Liko, Kristin Folmert, Christian Manz, Carol V. Robinson, Rainer Haag, Kevin Pagel
Published in: Chemical Science, Issue 11/13, 2020, Page(s) 3538-3546, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0sc01022g

The importance of the membrane for biophysical measurements

Author(s): Dror S. Chorev, Carol V. Robinson
Published in: Nature Chemical Biology, Issue 16/12, 2020, Page(s) 1285-1292, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-020-0574-1

Ligand binding to a G protein–coupled receptor captured in a mass spectrometer

Author(s): Hsin-Yung Yen, Jonathan T. S. Hopper, Idlir Liko, Timothy M. Allison, Ya Zhu, Dejian Wang, Monika Stegmann, Shabaz Mohammed, Beili Wu, Carol V. Robinson
Published in: Science Advances, Issue 3/6, 2017, Page(s) e1701016, ISSN 2375-2548
Publisher: AAAS
DOI: 10.1126/sciadv.1701016

Identifying key membrane protein lipid interactions using mass spectrometry

Author(s): Kallol Gupta, Jingwen Li, Idlir Liko, Joseph Gault, Cherine Bechara, Di Wu, Jonathan T S Hopper, Kevin Giles, Justin L P Benesch, Carol V Robinson
Published in: Nature Protocols, Issue 13/5, 2018, Page(s) 1106-1120, ISSN 1754-2189
Publisher: Nature Publishing Group
DOI: 10.1038/nprot.2018.014

Native Desorption Electrospray Ionization Liberates Soluble and Membrane Protein Complexes from Surfaces

Author(s): Stephen Ambrose, Nicholas G. Housden, Kallol Gupta, Jieyuan Fan, Paul White, Hsin-Yung Yen, Julien Marcoux, Colin Kleanthous, Jonathan T. S. Hopper, Carol V. Robinson
Published in: Angewandte Chemie International Edition, Issue 56/46, 2017, Page(s) 14463-14468, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201704849

PtdIns(4,5)P2 stabilizes active states of GPCRs and enhances selectivity of G-protein coupling

Author(s): Hsin-Yung Yen, Kin Kuan Hoi, Idlir Liko, George Hedger, Michael R. Horrell, Wanling Song, Di Wu, Philipp Heine, Tony Warne, Yang Lee, Byron Carpenter, Andreas Plückthun, Christopher G. Tate, Mark S. P. Sansom, Carol V. Robinson
Published in: Nature, Issue 559/7714, 2018, Page(s) 423-427, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-018-0325-6

Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry

Author(s): Dror S. Chorev, Lindsay A. Baker, Di Wu, Victoria Beilsten-Edmands, Sarah L. Rouse, Tzviya Zeev-Ben-Mordehai, Chimari Jiko, Firdaus Samsudin, Christoph Gerle, Syma Khalid, Alastair G. Stewart, Stephen J. Matthews, Kay Grünewald, Carol V. Robinson
Published in: Science, Issue 362/6416, 2018, Page(s) 829-834, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.aau0976

The role of interfacial lipids in stabilizing membrane protein oligomers

Author(s): Kallol Gupta, Joseph A. C. Donlan, Jonathan T. S. Hopper, Povilas Uzdavinys, Michael Landreh, Weston B. Struwe, David Drew, Andrew J. Baldwin, Phillip J. Stansfeld, Carol V. Robinson
Published in: Nature, Issue 541/7637, 2017, Page(s) 421-424, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/nature20820

Integrating mass spectrometry with MD simulations reveals the role of lipids in Na+/H+ antiporters

Author(s): Michael Landreh, Erik G. Marklund, Povilas Uzdavinys, Matteo T. Degiacomi, Mathieu Coincon, Joseph Gault, Kallol Gupta, Idlir Liko, Justin L. P. Benesch, David Drew, Carol V. Robinson
Published in: Nature Communications, Issue 8, 2017, Page(s) 13993, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/ncomms13993

From molecular chaperones to membrane motors: through the lens of a mass spectrometrist

Author(s): Carol V. Robinson
Published in: Biochemical Society Transactions, Issue 45/1, 2017, Page(s) 251-260, ISSN 0300-5127
Publisher: Portland Press, Ltd.
DOI: 10.1042/bst20160395

Structure of the Fanconi anaemia monoubiquitin ligase complex

Author(s): Shabih Shakeel, Eeson Rajendra, Pablo Alcón, Francis O’Reilly, Dror S. Chorev, Sarah Maslen, Gianluca Degliesposti, Christopher J. Russo, Shaoda He, Chris H. Hill, J. Mark Skehel, Sjors H. W. Scheres, Ketan J. Patel, Juri Rappsilber, Carol V. Robinson, Lori A. Passmore
Published in: Nature, Issue 575/7781, 2019, Page(s) 234-237, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-019-1703-4

Direct observation of the influence of cardiolipin and antibiotics on lipid II binding to MurJ

Author(s): Jani Reddy Bolla, Joshua B. Sauer, Di Wu, Shahid Mehmood, Timothy M. Allison, Carol V. Robinson
Published in: Nature Chemistry, Issue 10/3, 2018, Page(s) 363-371, ISSN 1755-4330
Publisher: Nature Publishing Group
DOI: 10.1038/nchem.2919