Elucidating natural bilayer lipid environments

Informazioni relative al progetto

ENABLE

ID dell’accordo di sovvenzione: 695511

DOI 10.3030/695511

Progetto chiuso

Data della firma CE 17 Maggio 2016

Data di avvio 1 Giugno 2016

Data di completamento 31 Maggio 2021

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale € 2 481 744,00

Contributo UE € 2 481 744,00

Coordinato da

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
United Kingdom

Pubblicazioni

Peer reviewed articles (22)

N-glycan microheterogeneity regulates interactions of plasma proteins

Autori: Di Wu, Weston B. Struwe, David J. Harvey, Michael A. J. Ferguson, Carol V. Robinson
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 115/35, 2018, Pagina/e 8763-8768, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.1807439115

Allosteric Inhibition of the SARS‐CoV‐2 Main Protease: Insights from Mass Spectrometry Based Assays**

Autori: Tarick J. El‐Baba, Corinne A. Lutomski, Anastassia L. Kantsadi, Tika R. Malla, Tobias John, Victor Mikhailov, Jani R. Bolla, Christopher J. Schofield, Nicole Zitzmann, Ioannis Vakonakis, Carol V. Robinson
Pubblicato in: Angewandte Chemie International Edition, Numero 59/52, 2020, Pagina/e 23544-23548, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202010316

Dynamics of an LPS translocon induced by substrate and an antimicrobial peptide

Autori: Francesco Fiorentino, Joshua B. Sauer, Xingyu Qiu, Robin A. Corey, C. Keith Cassidy, Benjamin Mynors-Wallis, Shahid Mehmood, Jani R. Bolla, Phillip J. Stansfeld, Carol V. Robinson
Pubblicato in: Nature Chemical Biology, Numero 17/2, 2021, Pagina/e 187-195, ISSN 1552-4450
Editore: Nature Publishing Group
DOI: 10.1038/s41589-020-00694-2

Structure of native lens connexin 46/50 intercellular channels by cryo-EM

Autori: Janette B. Myers, Bassam G. Haddad, Susan E. O’Neill, Dror S. Chorev, Craig C. Yoshioka, Carol V. Robinson, Daniel M. Zuckerman, Steve L. Reichow
Pubblicato in: Nature, Numero 564/7736, 2018, Pagina/e 372-377, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/s41586-018-0786-7

The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes

Autori: Dror S. Chorev, Haiping Tang, Sarah L. Rouse, Jani Reddy Bolla, Andriko von Kügelgen, Lindsay A. Baker, Di Wu, Joseph Gault, Kay Grünewald, Tanmay A. M. Bharat, Stephen J. Matthews, Carol V. Robinson
Pubblicato in: Nature Protocols, Numero 15/5, 2020, Pagina/e 1690-1706, ISSN 1754-2189
Editore: Nature Publishing Group
DOI: 10.1038/s41596-020-0303-y

Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins

Autori: Joseph Gault, Idlir Liko, Michael Landreh, Denis Shutin, Jani Reddy Bolla, Damien Jefferies, Mark Agasid, Hsin-Yung Yen, Marcus J. G. W. Ladds, David P. Lane, Syma Khalid, Christopher Mullen, Philip M. Remes, Romain Huguet, Graeme McAlister, Michael Goodwin, Rosa Viner, John E.P. Syka, Carol V. Robinson
Pubblicato in: Nature Methods, Numero 17/5, 2020, Pagina/e 505-508, ISSN 1548-7091
Editore: Nature Publishing Group
DOI: 10.1038/s41592-020-0821-0

Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors

Autori: Leonhard H. Urner, Idlir Liko, Hsin-Yung Yen, Kin-Kuan Hoi, Jani Reddy Bolla, Joseph Gault, Fernando Gonçalves Almeida, Marc-Philip Schweder, Denis Shutin, Svenja Ehrmann, Rainer Haag, Carol V. Robinson, Kevin Pagel
Pubblicato in: Nature Communications, Numero 11/1, 2020, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-020-14424-8

In Situ Structure of an Intact Lipopolysaccharide-Bound Bacterial Surface Layer

Autori: Andriko von Kügelgen, Haiping Tang, Gail G. Hardy, Danguole Kureisaite-Ciziene, Yves V. Brun, Phillip J. Stansfeld, Carol V. Robinson, Tanmay A.M. Bharat
Pubblicato in: Cell, Numero 180/2, 2020, Pagina/e 348-358.e15, ISSN 0092-8674
Editore: Cell Press
DOI: 10.1016/j.cell.2019.12.006

Phospho‐regulation, nucleotide binding and ion access control in potassium‐chloride cotransporters

Autori: Gamma Chi, Rebecca Ebenhoch, Henry Man, Haiping Tang, Laurence E Tremblay, Gabriella Reggiano, Xingyu Qiu, Tina Bohstedt, Idlir Liko, Fernando G Almeida, Alexandre P Garneau, Dong Wang, Gavin McKinley, Christophe P Moreau, Kiran D Bountra, Patrizia Abrusci, Shubhashish M M Mukhopadhyay, Alejandra Fernandez‐Cid, Samira Slimani, Julie L Lavoie, Nicola A Burgess‐Brown, Ben Tehan, Frank DiMaio, Al
Pubblicato in: The EMBO Journal, 2021, ISSN 0261-4189
Editore: Nature Publishing Group
DOI: 10.15252/embj.2020107294

Dendritic Oligoglycerol Regioisomer Mixtures and Their Utility for Membrane Protein Research

Autori: Leonhard H. Urner, Katharina Goltsche, Marleen Selent, Idlir Liko, Marc‐Philip Schweder, Carol V. Robinson, Kevin Pagel, Rainer Haag
Pubblicato in: Chemistry – A European Journal, Numero 27/7, 2021, Pagina/e 2537-2542, ISSN 0947-6539
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202003991

A new azobenzene-based design strategy for detergents in membrane protein research

Autori: Leonhard H. Urner, Maiko Schulze, Yasmine B. Maier, Waldemar Hoffmann, Stephan Warnke, Idlir Liko, Kristin Folmert, Christian Manz, Carol V. Robinson, Rainer Haag, Kevin Pagel
Pubblicato in: Chemical Science, Numero 11/13, 2020, Pagina/e 3538-3546, ISSN 2041-6520
Editore: Royal Society of Chemistry
DOI: 10.1039/d0sc01022g

The importance of the membrane for biophysical measurements

Autori: Dror S. Chorev, Carol V. Robinson
Pubblicato in: Nature Chemical Biology, Numero 16/12, 2020, Pagina/e 1285-1292, ISSN 1552-4450
Editore: Nature Publishing Group
DOI: 10.1038/s41589-020-0574-1

Ligand binding to a G protein–coupled receptor captured in a mass spectrometer

Autori: Hsin-Yung Yen, Jonathan T. S. Hopper, Idlir Liko, Timothy M. Allison, Ya Zhu, Dejian Wang, Monika Stegmann, Shabaz Mohammed, Beili Wu, Carol V. Robinson
Pubblicato in: Science Advances, Numero 3/6, 2017, Pagina/e e1701016, ISSN 2375-2548
Editore: AAAS
DOI: 10.1126/sciadv.1701016

Identifying key membrane protein lipid interactions using mass spectrometry

Autori: Kallol Gupta, Jingwen Li, Idlir Liko, Joseph Gault, Cherine Bechara, Di Wu, Jonathan T S Hopper, Kevin Giles, Justin L P Benesch, Carol V Robinson
Pubblicato in: Nature Protocols, Numero 13/5, 2018, Pagina/e 1106-1120, ISSN 1754-2189
Editore: Nature Publishing Group
DOI: 10.1038/nprot.2018.014

Native Desorption Electrospray Ionization Liberates Soluble and Membrane Protein Complexes from Surfaces

Autori: Stephen Ambrose, Nicholas G. Housden, Kallol Gupta, Jieyuan Fan, Paul White, Hsin-Yung Yen, Julien Marcoux, Colin Kleanthous, Jonathan T. S. Hopper, Carol V. Robinson
Pubblicato in: Angewandte Chemie International Edition, Numero 56/46, 2017, Pagina/e 14463-14468, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201704849

PtdIns(4,5)P2 stabilizes active states of GPCRs and enhances selectivity of G-protein coupling

Autori: Hsin-Yung Yen, Kin Kuan Hoi, Idlir Liko, George Hedger, Michael R. Horrell, Wanling Song, Di Wu, Philipp Heine, Tony Warne, Yang Lee, Byron Carpenter, Andreas Plückthun, Christopher G. Tate, Mark S. P. Sansom, Carol V. Robinson
Pubblicato in: Nature, Numero 559/7714, 2018, Pagina/e 423-427, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/s41586-018-0325-6

Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry

Autori: Dror S. Chorev, Lindsay A. Baker, Di Wu, Victoria Beilsten-Edmands, Sarah L. Rouse, Tzviya Zeev-Ben-Mordehai, Chimari Jiko, Firdaus Samsudin, Christoph Gerle, Syma Khalid, Alastair G. Stewart, Stephen J. Matthews, Kay Grünewald, Carol V. Robinson
Pubblicato in: Science, Numero 362/6416, 2018, Pagina/e 829-834, ISSN 0036-8075
Editore: American Association for the Advancement of Science
DOI: 10.1126/science.aau0976

The role of interfacial lipids in stabilizing membrane protein oligomers

Autori: Kallol Gupta, Joseph A. C. Donlan, Jonathan T. S. Hopper, Povilas Uzdavinys, Michael Landreh, Weston B. Struwe, David Drew, Andrew J. Baldwin, Phillip J. Stansfeld, Carol V. Robinson
Pubblicato in: Nature, Numero 541/7637, 2017, Pagina/e 421-424, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/nature20820

Integrating mass spectrometry with MD simulations reveals the role of lipids in Na+/H+ antiporters

Autori: Michael Landreh, Erik G. Marklund, Povilas Uzdavinys, Matteo T. Degiacomi, Mathieu Coincon, Joseph Gault, Kallol Gupta, Idlir Liko, Justin L. P. Benesch, David Drew, Carol V. Robinson
Pubblicato in: Nature Communications, Numero 8, 2017, Pagina/e 13993, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/ncomms13993

From molecular chaperones to membrane motors: through the lens of a mass spectrometrist

Autori: Carol V. Robinson
Pubblicato in: Biochemical Society Transactions, Numero 45/1, 2017, Pagina/e 251-260, ISSN 0300-5127
Editore: Portland Press, Ltd.
DOI: 10.1042/bst20160395

Structure of the Fanconi anaemia monoubiquitin ligase complex

Autori: Shabih Shakeel, Eeson Rajendra, Pablo Alcón, Francis O’Reilly, Dror S. Chorev, Sarah Maslen, Gianluca Degliesposti, Christopher J. Russo, Shaoda He, Chris H. Hill, J. Mark Skehel, Sjors H. W. Scheres, Ketan J. Patel, Juri Rappsilber, Carol V. Robinson, Lori A. Passmore
Pubblicato in: Nature, Numero 575/7781, 2019, Pagina/e 234-237, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/s41586-019-1703-4

Direct observation of the influence of cardiolipin and antibiotics on lipid II binding to MurJ

Autori: Jani Reddy Bolla, Joshua B. Sauer, Di Wu, Shahid Mehmood, Timothy M. Allison, Carol V. Robinson
Pubblicato in: Nature Chemistry, Numero 10/3, 2018, Pagina/e 363-371, ISSN 1755-4330
Editore: Nature Publishing Group
DOI: 10.1038/nchem.2919