An integrated mulTidisciplinary appRoach towards a new generAtIon of aNtibiotics: Targeting function and cross-talk of bacterial Envelope proTein machineries

Deliverables

Documents, reports (1)

Scientific publications

Min. 45 publications in peer-reviewed journals

Other (2)

Industry course

Industry course

SB and SAB established

SB and SAB established

Websites, patent fillings, videos etc. (2)

Website

Website www.train2target.eu

Science Slams

Science Slams

Publications

Peer reviewed articles (27)

Effective approaches to discover new microbial metabolites in a large strain library.

Author(s): Mitja M Zdouc, Marianna Iorio, Kristiina Vind, Matteo Simone, Stefania Serina, Cristina Brunati, Paolo Monciardini, Arianna Tocchetti, Guadalupe S Zarazúa, Max Crüsemann, Sonia I Maffioli, Margherita Sosio, Stefano Donadio
Published in: Journal of Industrial Microbiology and Biotechnology, 2021, ISSN 1476-5535
Publisher: Springer-Nature
DOI: 10.1093/jimb/kuab017

The active repertoire of Escherichia coli peptidoglycan amidases varies with physiochemical environment

Author(s): Mueller, Elizabeth A; Iken, Abbygail G; Öztürk, Mehmet; Winkle, Matthias; Schmitz, Mirko; Vollmer, Waldemar; Di Ventura, Barbara; Levin, Petra Anne
Published in: Molecular Microbiology, 2021, ISSN 0950-382X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/mmi.14711

Carbohydrate-based adjuvants

Author(s): Pilar Garcia-Vello, Immacolata Speciale, Fabrizio Chiodo, Antonio Molinaro, Cristina De Castro
Published in: Drug Discovery Today: Technologies, 35-36, 2020, Page(s) 57-68, ISSN 1740-6749
Publisher: Elsevier BV
DOI: 10.1016/j.ddtec.2020.09.005

A biaryl-linked tripeptide from Planomonospora reveals a widespread class of minimal RiPP gene clusters

Author(s): Mitja M. Zdouc, Mohammad M. Alanjary, Guadalupe S. Zarazúa, Sonia I. Maffioli, Max Crüsemann, Marnix H. Medema, Stefano Donadio, Margherita Sosio
Published in: Cell Chemical Biology, 2020, ISSN 2451-9456
Publisher: Cell Chemical Biology
DOI: 10.1016/j.chembiol.2020.11.009

"Detection of in vivo Protein Interactions in All Bacterial Components by Förster Resonance Energy Transfer with the Superfolder mTurquoise2 ox-mNeongreen FRET Pair"

Author(s): Nils Meiresonne, Elisa Consoli, Laureen Mertens, Tanneke den Blaauwen
Published in: BIO-PROTOCOL, 9/23, 2019, ISSN 2331-8325
Publisher: Bio-protocol
DOI: 10.21769/bioprotoc.3448

Lipoproteins in Gram-negative bacteria: new insights into their biogenesis, subcellular targeting and functional roles

Author(s): Jessica El Rayes, Raquel Rodríguez-Alonso, Jean-François Collet
Published in: Current Opinion in Microbiology, 61, 2021, Page(s) 25-34, ISSN 1369-5274
Publisher: Elsevier BV
DOI: 10.1016/j.mib.2021.02.003

Structural features and immunological perception of the cell surface glycans of Lactobacillus plantarum: a novel rhamnose-rich polysaccharide and teichoic acids

Author(s): Pilar Garcia-Vello, Garima Sharma, Immacolata Speciale, Antonio Molinaro, Sin-Hyeog Im, Cristina De Castro
Published in: Carbohydrate Polymers, 233, 2020, Page(s) 115857, ISSN 0144-8617
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.carbpol.2020.115857

The Lpt ABC transporter for lipopolysaccharide export to the cell surface

Author(s): Paola Sperandeo, Alessandra M. Martorana, Alessandra Polissi
Published in: Research in Microbiology, 170/8, 2019, Page(s) 366-373, ISSN 0923-2508
Publisher: Elsevier BV
DOI: 10.1016/j.resmic.2019.07.005

Planomonospora : A Metabolomics Perspective on an Underexplored Actinobacteria Genus

Author(s): Mitja M. Zdouc, Marianna Iorio, Sonia I. Maffioli, Max Crüsemann, Stefano Donadio, Margherita Sosio
Published in: Journal of Natural Products, 84/2, 2021, Page(s) 204-219, ISSN 0163-3864
Publisher: American Chemical Society
DOI: 10.1021/acs.jnatprod.0c00807

In vitro activity of rifabutin against 293 contemporary carbapenem-resistant Acinetobacter baumannii clinical isolates and characterization of rifabutin mode of action and resistance mechanisms

Author(s): Vincent Trebosc, Birgit Schellhorn, Julian Schill, Valentina Lucchini, Jacqueline Bühler, Marilyne Bourotte, Jonathan J Butcher, Marc Gitzinger, Sergio Lociuro, Christian Kemmer, Glenn E Dale
Published in: Journal of Antimicrobial Chemotherapy, 75/12, 2020, Page(s) 3552-3562, ISSN 0305-7453
Publisher: Oxford University Press
DOI: 10.1093/jac/dkaa370

The cell cycle regulator GpsB functions as cytosolic adaptor for multiple cell wall enzymes

Author(s): Robert M. Cleverley, Zoe J. Rutter, Jeanine Rismondo, Federico Corona, Ho-Ching Tiffany Tsui, Fuad A. Alatawi, Richard A. Daniel, Sven Halbedel, Orietta Massidda, Malcolm E. Winkler, Richard J. Lewis
Published in: Nature Communications, 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-08056-2

Thanatin Impairs Lipopolysaccharide Transport Complex Assembly by Targeting LptC–LptA Interaction and Decreasing LptA Stability

Author(s): Elisabete C. C. M. Moura, Tiago Baeta, Alessandra Romanelli, Cedric Laguri, Alessandra M. Martorana, Emanuela Erba, Jean-Pierre Simorre, Paola Sperandeo, Alessandra Polissi
Published in: Frontiers in Microbiology, 11, 2020, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2020.00909

ActS activates peptidoglycan amidases during outer membrane stress in Escherichia coli

Author(s): Carlos K. Gurnani Serrano, Matthias Winkle, Alessandra M. Martorana, Jacob Biboy, Niccolo Morè, Patrick Moynihan, Manuel Banzhaf, Waldemar Vollmer, Alessandra Polissi
Published in: Molecular Microbiology, 2021, ISSN 0950-382X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/mmi.14712

The lipopolysaccharide transport (Lpt) machinery: A nonconventional transporter for lipopolysaccharide assembly at the outer membrane of Gram-negative bacteria

Author(s): Paola Sperandeo, Alessandra M. Martorana, Alessandra Polissi
Published in: Journal of Biological Chemistry, 292/44, 2017, Page(s) 17981-17990, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.R117.802512

Copper inhibits peptidoglycan LD-transpeptidases suppressing β-lactam resistance due to bypass of penicillin-binding proteins

Author(s): Katharina Peters, Manuel Pazos, Zainab Edoo, Jean-Emmanuel Hugonnet, Alessandra M. Martorana, Alessandra Polissi, Michael S. VanNieuwenhze, Michel Arthur, Waldemar Vollmer
Published in: Proceedings of the National Academy of Sciences, 115/42, 2018, Page(s) 10786-10791, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1809285115

Interaction of lipopolysaccharides at intermolecular sites of the periplasmic Lpt transport assembly

Author(s): Cedric Laguri, Paola Sperandeo, Kevin Pounot, Isabel Ayala, Alba Silipo, Catherine M. Bougault, Antonio Molinaro, Alessandra Polissi, Jean-Pierre Simorre
Published in: Scientific Reports, 7/1, 2017, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-017-10136-0

Superfolder mTurquoise2 ox optimized for the bacterial periplasm allows high efficiency in vivo FRET of cell division antibiotic targets

Author(s): Nils Y. Meiresonne, Elisa Consoli, Laureen M. Y. Mertens, Anna Chertkova, Joachim Goedhart, Tanneke den Blaauwen
Published in: Molecular Microbiology, 2019, ISSN 0950-382X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/mmi.14206

Solid State NMR Studies of Intact Lipopolysaccharide Endotoxin

Author(s): Cedric Laguri, Alba Silipo, Alessandra M. Martorana, Paul Schanda, Roberta Marchetti, Alessandra Polissi, Antonio Molinaro, Jean-Pierre Simorre
Published in: ACS Chemical Biology, 13/8, 2018, Page(s) 2106-2113, ISSN 1554-8929
Publisher: American Chemical Society
DOI: 10.1021/acschembio.8b00271

Peptidoglycan Remodeling Enables Escherichia coli To Survive Severe Outer Membrane Assembly Defect

Author(s): Niccolò Morè, Alessandra M. Martorana, Jacob Biboy, Christian Otten, Matthias Winkle, Carlos K. Gurnani Serrano, Alejandro Montón Silva, Lisa Atkinson, Hamish Yau, Eefjan Breukink, Tanneke den Blaauwen, Waldemar Vollmer, Alessandra Polissi
Published in: mBio, 10/1, 2019, ISSN 2150-7511
Publisher: American Society for Microbiology
DOI: 10.1128/mbio.02729-18

Structural insight into the formation of lipoprotein-β-barrel complexes

Author(s): Raquel Rodríguez-Alonso, Juliette Létoquart, Van Son Nguyen, Gwennaelle Louis, Antonio N. Calabrese, Bogdan I. Iorga, Sheena E. Radford, Seung-Hyun Cho, Han Remaut, Jean-François Collet
Published in: Nature Chemical Biology, 16/9, 2020, Page(s) 1019-1025, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-020-0575-0

Structure of the O‐Antigen and the Lipid A from the Lipopolysaccharide of Fusobacterium nucleatum ATCC 51191

Author(s): Pilar Garcia‐Vello, Flaviana Di Lorenzo, Dimitra Lamprinaki, Anna Notaro, Immacolata Speciale, Antonio Molinaro, Nathalie Juge, Cristina De Castro
Published in: ChemBioChem, 22/7, 2021, Page(s) 1252-1260, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000751

The Escherichia coli Outer Membrane β-Barrel Assembly Machinery (BAM) Anchors the Peptidoglycan Layer by Spanning It with All Subunits

Author(s): Elisa Consoli, Jean-François Collet, Tanneke den Blaauwen
Published in: International Journal of Molecular Sciences, 22/4, 2021, Page(s) 1853, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22041853

Blocks in the pseudouridimycin pathway unlock hidden metabolites in the Streptomyces producer strain

Author(s): Marianna Iorio, Sahar Davatgarbenam, Stefania Serina, Paolo Criscenzo, Mitja M. Zdouc, Matteo Simone, Sonia I. Maffioli, Richard H. Ebright, Stefano Donadio, Margherita Sosio
Published in: Scientific Reports, 11/1, 2021, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-84833-2

Dissecting Colistin Resistance Mechanisms in Extensively Drug-Resistant Acinetobacter baumannii Clinical Isolates

Author(s): Vincent Trebosc, Sarah Gartenmann, Marcus Tötzl, Valentina Lucchini, Birgit Schellhorn, Michel Pieren, Sergio Lociuro, Marc Gitzinger, Marcel Tigges, Dirk Bumann, Christian Kemmer
Published in: mBio, 10/4, 2019, ISSN 2150-7511
Publisher: American Society for Microbiology
DOI: 10.1128/mbio.01083-19

MreC and MreD balance the interaction between the elongasome proteins PBP2 and RodA

Author(s): Xiaolong Liu, Jacob Biboy, Elisa Consoli, Waldemar Vollmer, Tanneke den Blaauwen
Published in: Plos Genetics, 16(12), 2020, Page(s) e1009276, ISSN 1553-7390
Publisher: Public Library of Science
DOI: 10.1371/journal.pgen.1009276

Fat Matters for Bugs: How Lipids and Lipid Modifications Make the Difference in Bacterial Life

Author(s): Paola Sperandeo, Alessandra Polissi, Emma De Fabiani
Published in: European Journal of Lipid Science and Technology, 121/10, 2019, Page(s) 1900204, ISSN 1438-7697
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/ejlt.201900204

Mutation and Suppressor Analysis of the Essential Lipopolysaccharide Transport Protein LptA Reveals Strategies To Overcome Severe Outer Membrane Permeability Defects in Escherichia coli

Author(s): Federica A. Falchi, Elisa A. Maccagni, Simone Puccio, Clelia Peano, Cristina De Castro, Angelo Palmigiano, Domenico Garozzo, Alessandra M. Martorana, Alessandra Polissi, Gianni Dehò, Paola Sperandeo
Published in: Journal of Bacteriology, 200/2, 2018, ISSN 0021-9193
Publisher: American Society for Microbiology
DOI: 10.1128/jb.00487-17

Other (3)

Structure-function analyses of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation

Author(s): J. A. Bryant, F. C. Morris, T. J. Knowles, R. Maderbocus, E. Heinz, G. Boelter, D. Alodaini, A. Colyer, P. J. Wotherspoon, K. A. Staunton, M. Jeeves, D. F. Browning, Y. R. Sevastsyanovich, T. J. Wells, A. E. Rossiter, V. N. Bavro, P. Sridhar, D. G. Ward, Z-S. Chong, C. Icke, A. Teo, S-S. Chng, View ORCID ProfileD. I. Roper, T. Lithgow, A. F. Cunningham, M. Banzhaf, M. Overduin, I. R. Henderson
Published in: BioRxiv, 2020
Publisher: BioRxiv
DOI: 10.1101/2020.08.10.244616

Disorder is a critical component of lipoprotein sorting in Gram-negative bacteria

Author(s): Jessica El Rayes, Joanna Szewczyk, Michael Deghelt, André Matagne, Bogdan I. Iorga, Seung-Hyun Cho, Jean-François Collet
Published in: BioRxiv, 2021
Publisher: BioRxiv
DOI: 10.1101/2021.01.05.425367

Metabolomic investigation of the pseudouridimycin producer, a prolific streptomycete

Author(s): Iorio, M., Davatgarbenam, S., Serina, S., Criscenzo, P., Zdouc, M.M., Simone, M., Maffioli, S.I., Ebright, R.H., Donadio, S. and Sosio, M.
Published in: BioRxiv, 5. November 2020, 2020
Publisher: BioRxiv
DOI: 10.1101/2020.11.05.369249

Thesis dissertations (1)

Antibiotic drug development

Author(s): Matias, C. S. S., Sommer, M. O. A., Ingmer, H., Weber, T., Polissi, A., & Olsen, J. E.
Published in: 2020
Publisher: Technical University of Denmark