An integrated mulTidisciplinary appRoach towards a new generAtIon of aNtibiotics: Targeting function and cross-talk of bacterial Envelope proTein machineries

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Min. 45 publications in peer-reviewed journals

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SB and SAB established

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Publications

Effective approaches to discover new microbial metabolites in a large strain library.

Auteurs: Mitja M Zdouc, Marianna Iorio, Kristiina Vind, Matteo Simone, Stefania Serina, Cristina Brunati, Paolo Monciardini, Arianna Tocchetti, Guadalupe S Zarazúa, Max Crüsemann, Sonia I Maffioli, Margherita Sosio, Stefano Donadio
Publié dans: Journal of Industrial Microbiology and Biotechnology, 2021, ISSN 1476-5535
Éditeur: Springer-Nature
DOI: 10.1093/jimb/kuab017

The active repertoire of Escherichia coli peptidoglycan amidases varies with physiochemical environment

Auteurs: Mueller, Elizabeth A; Iken, Abbygail G; Öztürk, Mehmet; Winkle, Matthias; Schmitz, Mirko; Vollmer, Waldemar; Di Ventura, Barbara; Levin, Petra Anne
Publié dans: Molecular Microbiology, 2021, ISSN 0950-382X
Éditeur: Blackwell Publishing Inc.
DOI: 10.1111/mmi.14711

Carbohydrate-based adjuvants

Auteurs: Pilar Garcia-Vello, Immacolata Speciale, Fabrizio Chiodo, Antonio Molinaro, Cristina De Castro
Publié dans: Drug Discovery Today: Technologies, Numéro 35-36, 2020, Page(s) 57-68, ISSN 1740-6749
Éditeur: Elsevier BV
DOI: 10.1016/j.ddtec.2020.09.005

A biaryl-linked tripeptide from Planomonospora reveals a widespread class of minimal RiPP gene clusters

Auteurs: Mitja M. Zdouc, Mohammad M. Alanjary, Guadalupe S. Zarazúa, Sonia I. Maffioli, Max Crüsemann, Marnix H. Medema, Stefano Donadio, Margherita Sosio
Publié dans: Cell Chemical Biology, 2020, ISSN 2451-9456
Éditeur: Cell Chemical Biology
DOI: 10.1016/j.chembiol.2020.11.009

"Detection of in vivo Protein Interactions in All Bacterial Components by Förster Resonance Energy Transfer with the Superfolder mTurquoise2 ox-mNeongreen FRET Pair"

Auteurs: Nils Meiresonne, Elisa Consoli, Laureen Mertens, Tanneke den Blaauwen
Publié dans: BIO-PROTOCOL, Numéro 9/23, 2019, ISSN 2331-8325
Éditeur: Bio-protocol
DOI: 10.21769/bioprotoc.3448

Lipoproteins in Gram-negative bacteria: new insights into their biogenesis, subcellular targeting and functional roles

Auteurs: Jessica El Rayes, Raquel Rodríguez-Alonso, Jean-François Collet
Publié dans: Current Opinion in Microbiology, Numéro 61, 2021, Page(s) 25-34, ISSN 1369-5274
Éditeur: Elsevier BV
DOI: 10.1016/j.mib.2021.02.003

Structural features and immunological perception of the cell surface glycans of Lactobacillus plantarum: a novel rhamnose-rich polysaccharide and teichoic acids

Auteurs: Pilar Garcia-Vello, Garima Sharma, Immacolata Speciale, Antonio Molinaro, Sin-Hyeog Im, Cristina De Castro
Publié dans: Carbohydrate Polymers, Numéro 233, 2020, Page(s) 115857, ISSN 0144-8617
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.carbpol.2020.115857

The Lpt ABC transporter for lipopolysaccharide export to the cell surface

Auteurs: Paola Sperandeo, Alessandra M. Martorana, Alessandra Polissi
Publié dans: Research in Microbiology, Numéro 170/8, 2019, Page(s) 366-373, ISSN 0923-2508
Éditeur: Elsevier BV
DOI: 10.1016/j.resmic.2019.07.005

Planomonospora : A Metabolomics Perspective on an Underexplored Actinobacteria Genus

Auteurs: Mitja M. Zdouc, Marianna Iorio, Sonia I. Maffioli, Max Crüsemann, Stefano Donadio, Margherita Sosio
Publié dans: Journal of Natural Products, Numéro 84/2, 2021, Page(s) 204-219, ISSN 0163-3864
Éditeur: American Chemical Society
DOI: 10.1021/acs.jnatprod.0c00807

In vitro activity of rifabutin against 293 contemporary carbapenem-resistant Acinetobacter baumannii clinical isolates and characterization of rifabutin mode of action and resistance mechanisms

Auteurs: Vincent Trebosc, Birgit Schellhorn, Julian Schill, Valentina Lucchini, Jacqueline Bühler, Marilyne Bourotte, Jonathan J Butcher, Marc Gitzinger, Sergio Lociuro, Christian Kemmer, Glenn E Dale
Publié dans: Journal of Antimicrobial Chemotherapy, Numéro 75/12, 2020, Page(s) 3552-3562, ISSN 0305-7453
Éditeur: Oxford University Press
DOI: 10.1093/jac/dkaa370

The cell cycle regulator GpsB functions as cytosolic adaptor for multiple cell wall enzymes

Auteurs: Robert M. Cleverley, Zoe J. Rutter, Jeanine Rismondo, Federico Corona, Ho-Ching Tiffany Tsui, Fuad A. Alatawi, Richard A. Daniel, Sven Halbedel, Orietta Massidda, Malcolm E. Winkler, Richard J. Lewis
Publié dans: Nature Communications, Numéro 10/1, 2019, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-018-08056-2

Thanatin Impairs Lipopolysaccharide Transport Complex Assembly by Targeting LptC–LptA Interaction and Decreasing LptA Stability

Auteurs: Elisabete C. C. M. Moura, Tiago Baeta, Alessandra Romanelli, Cedric Laguri, Alessandra M. Martorana, Emanuela Erba, Jean-Pierre Simorre, Paola Sperandeo, Alessandra Polissi
Publié dans: Frontiers in Microbiology, Numéro 11, 2020, ISSN 1664-302X
Éditeur: Frontiers Media
DOI: 10.3389/fmicb.2020.00909

ActS activates peptidoglycan amidases during outer membrane stress in Escherichia coli

Auteurs: Carlos K. Gurnani Serrano, Matthias Winkle, Alessandra M. Martorana, Jacob Biboy, Niccolo Morè, Patrick Moynihan, Manuel Banzhaf, Waldemar Vollmer, Alessandra Polissi
Publié dans: Molecular Microbiology, 2021, ISSN 0950-382X
Éditeur: Blackwell Publishing Inc.
DOI: 10.1111/mmi.14712

The lipopolysaccharide transport (Lpt) machinery: A nonconventional transporter for lipopolysaccharide assembly at the outer membrane of Gram-negative bacteria

Auteurs: Paola Sperandeo, Alessandra M. Martorana, Alessandra Polissi
Publié dans: Journal of Biological Chemistry, Numéro 292/44, 2017, Page(s) 17981-17990, ISSN 0021-9258
Éditeur: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.R117.802512

Copper inhibits peptidoglycan LD-transpeptidases suppressing β-lactam resistance due to bypass of penicillin-binding proteins

Auteurs: Katharina Peters, Manuel Pazos, Zainab Edoo, Jean-Emmanuel Hugonnet, Alessandra M. Martorana, Alessandra Polissi, Michael S. VanNieuwenhze, Michel Arthur, Waldemar Vollmer
Publié dans: Proceedings of the National Academy of Sciences, Numéro 115/42, 2018, Page(s) 10786-10791, ISSN 0027-8424
Éditeur: National Academy of Sciences
DOI: 10.1073/pnas.1809285115

Interaction of lipopolysaccharides at intermolecular sites of the periplasmic Lpt transport assembly

Auteurs: Cedric Laguri, Paola Sperandeo, Kevin Pounot, Isabel Ayala, Alba Silipo, Catherine M. Bougault, Antonio Molinaro, Alessandra Polissi, Jean-Pierre Simorre
Publié dans: Scientific Reports, Numéro 7/1, 2017, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-017-10136-0

Superfolder mTurquoise2 ox optimized for the bacterial periplasm allows high efficiency in vivo FRET of cell division antibiotic targets

Auteurs: Nils Y. Meiresonne, Elisa Consoli, Laureen M. Y. Mertens, Anna Chertkova, Joachim Goedhart, Tanneke den Blaauwen
Publié dans: Molecular Microbiology, 2019, ISSN 0950-382X
Éditeur: Blackwell Publishing Inc.
DOI: 10.1111/mmi.14206

Solid State NMR Studies of Intact Lipopolysaccharide Endotoxin

Auteurs: Cedric Laguri, Alba Silipo, Alessandra M. Martorana, Paul Schanda, Roberta Marchetti, Alessandra Polissi, Antonio Molinaro, Jean-Pierre Simorre
Publié dans: ACS Chemical Biology, Numéro 13/8, 2018, Page(s) 2106-2113, ISSN 1554-8929
Éditeur: American Chemical Society
DOI: 10.1021/acschembio.8b00271

Peptidoglycan Remodeling Enables Escherichia coli To Survive Severe Outer Membrane Assembly Defect

Auteurs: Niccolò Morè, Alessandra M. Martorana, Jacob Biboy, Christian Otten, Matthias Winkle, Carlos K. Gurnani Serrano, Alejandro Montón Silva, Lisa Atkinson, Hamish Yau, Eefjan Breukink, Tanneke den Blaauwen, Waldemar Vollmer, Alessandra Polissi
Publié dans: mBio, Numéro 10/1, 2019, ISSN 2150-7511
Éditeur: American Society for Microbiology
DOI: 10.1128/mbio.02729-18

Structural insight into the formation of lipoprotein-β-barrel complexes

Auteurs: Raquel Rodríguez-Alonso, Juliette Létoquart, Van Son Nguyen, Gwennaelle Louis, Antonio N. Calabrese, Bogdan I. Iorga, Sheena E. Radford, Seung-Hyun Cho, Han Remaut, Jean-François Collet
Publié dans: Nature Chemical Biology, Numéro 16/9, 2020, Page(s) 1019-1025, ISSN 1552-4450
Éditeur: Nature Publishing Group
DOI: 10.1038/s41589-020-0575-0

Structure of the O‐Antigen and the Lipid A from the Lipopolysaccharide of Fusobacterium nucleatum ATCC 51191

Auteurs: Pilar Garcia‐Vello, Flaviana Di Lorenzo, Dimitra Lamprinaki, Anna Notaro, Immacolata Speciale, Antonio Molinaro, Nathalie Juge, Cristina De Castro
Publié dans: ChemBioChem, Numéro 22/7, 2021, Page(s) 1252-1260, ISSN 1439-4227
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000751

The Escherichia coli Outer Membrane β-Barrel Assembly Machinery (BAM) Anchors the Peptidoglycan Layer by Spanning It with All Subunits

Auteurs: Elisa Consoli, Jean-François Collet, Tanneke den Blaauwen
Publié dans: International Journal of Molecular Sciences, Numéro 22/4, 2021, Page(s) 1853, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22041853

Blocks in the pseudouridimycin pathway unlock hidden metabolites in the Streptomyces producer strain

Auteurs: Marianna Iorio, Sahar Davatgarbenam, Stefania Serina, Paolo Criscenzo, Mitja M. Zdouc, Matteo Simone, Sonia I. Maffioli, Richard H. Ebright, Stefano Donadio, Margherita Sosio
Publié dans: Scientific Reports, Numéro 11/1, 2021, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-021-84833-2

Dissecting Colistin Resistance Mechanisms in Extensively Drug-Resistant Acinetobacter baumannii Clinical Isolates

Auteurs: Vincent Trebosc, Sarah Gartenmann, Marcus Tötzl, Valentina Lucchini, Birgit Schellhorn, Michel Pieren, Sergio Lociuro, Marc Gitzinger, Marcel Tigges, Dirk Bumann, Christian Kemmer
Publié dans: mBio, Numéro 10/4, 2019, ISSN 2150-7511
Éditeur: American Society for Microbiology
DOI: 10.1128/mbio.01083-19

MreC and MreD balance the interaction between the elongasome proteins PBP2 and RodA

Auteurs: Xiaolong Liu, Jacob Biboy, Elisa Consoli, Waldemar Vollmer, Tanneke den Blaauwen
Publié dans: Plos Genetics, Numéro 16(12), 2020, Page(s) e1009276, ISSN 1553-7390
Éditeur: Public Library of Science
DOI: 10.1371/journal.pgen.1009276

Fat Matters for Bugs: How Lipids and Lipid Modifications Make the Difference in Bacterial Life

Auteurs: Paola Sperandeo, Alessandra Polissi, Emma De Fabiani
Publié dans: European Journal of Lipid Science and Technology, Numéro 121/10, 2019, Page(s) 1900204, ISSN 1438-7697
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/ejlt.201900204

Mutation and Suppressor Analysis of the Essential Lipopolysaccharide Transport Protein LptA Reveals Strategies To Overcome Severe Outer Membrane Permeability Defects in Escherichia coli

Auteurs: Federica A. Falchi, Elisa A. Maccagni, Simone Puccio, Clelia Peano, Cristina De Castro, Angelo Palmigiano, Domenico Garozzo, Alessandra M. Martorana, Alessandra Polissi, Gianni Dehò, Paola Sperandeo
Publié dans: Journal of Bacteriology, Numéro 200/2, 2018, ISSN 0021-9193
Éditeur: American Society for Microbiology
DOI: 10.1128/jb.00487-17

Structure-function analyses of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation

Auteurs: J. A. Bryant, F. C. Morris, T. J. Knowles, R. Maderbocus, E. Heinz, G. Boelter, D. Alodaini, A. Colyer, P. J. Wotherspoon, K. A. Staunton, M. Jeeves, D. F. Browning, Y. R. Sevastsyanovich, T. J. Wells, A. E. Rossiter, V. N. Bavro, P. Sridhar, D. G. Ward, Z-S. Chong, C. Icke, A. Teo, S-S. Chng, View ORCID ProfileD. I. Roper, T. Lithgow, A. F. Cunningham, M. Banzhaf, M. Overduin, I. R. Henderson
Publié dans: BioRxiv, 2020
Éditeur: BioRxiv
DOI: 10.1101/2020.08.10.244616

Disorder is a critical component of lipoprotein sorting in Gram-negative bacteria

Auteurs: Jessica El Rayes, Joanna Szewczyk, Michael Deghelt, André Matagne, Bogdan I. Iorga, Seung-Hyun Cho, Jean-François Collet
Publié dans: BioRxiv, 2021
Éditeur: BioRxiv
DOI: 10.1101/2021.01.05.425367

Metabolomic investigation of the pseudouridimycin producer, a prolific streptomycete

Auteurs: Iorio, M., Davatgarbenam, S., Serina, S., Criscenzo, P., Zdouc, M.M., Simone, M., Maffioli, S.I., Ebright, R.H., Donadio, S. and Sosio, M.
Publié dans: BioRxiv, Numéro 5. November 2020, 2020
Éditeur: BioRxiv
DOI: 10.1101/2020.11.05.369249

Antibiotic drug development

Auteurs: Matias, C. S. S., Sommer, M. O. A., Ingmer, H., Weber, T., Polissi, A., & Olsen, J. E.
Publié dans: 2020
Éditeur: Technical University of Denmark

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