An integrated mulTidisciplinary appRoach towards a new generAtIon of aNtibiotics: Targeting function and cross-talk of bacterial Envelope proTein machineries

Informazioni relative al progetto

Train2Target

ID dell’accordo di sovvenzione: 721484

DOI

10.3030/721484

Progetto chiuso

Data della firma CE 31 Agosto 2016

Data di avvio 1 Gennaio 2017

Data di completamento 31 Dicembre 2020

Finanziato da

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Costo totale

€ 3 931 378,20

Contributo UE

€ 3 931 378,20

Coordinato da

UNIVERSITA DEGLI STUDI DI MILANO
Italy

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Documents, reports (1)

Scientific publications

Min. 45 publications in peer-reviewed journals

Other (2)

Industry course
SB and SAB established

Websites, patent fillings, videos etc. (2)

Website

Website www.train2target.eu

Science Slams

Pubblicazioni

Peer reviewed articles (27)

Effective approaches to discover new microbial metabolites in a large strain library.

Autori: Mitja M Zdouc, Marianna Iorio, Kristiina Vind, Matteo Simone, Stefania Serina, Cristina Brunati, Paolo Monciardini, Arianna Tocchetti, Guadalupe S Zarazúa, Max Crüsemann, Sonia I Maffioli, Margherita Sosio, Stefano Donadio
Pubblicato in: Journal of Industrial Microbiology and Biotechnology, 2021, ISSN 1476-5535
Editore: Springer-Nature
DOI: 10.1093/jimb/kuab017

The active repertoire of Escherichia coli peptidoglycan amidases varies with physiochemical environment

Autori: Mueller, Elizabeth A; Iken, Abbygail G; Öztürk, Mehmet; Winkle, Matthias; Schmitz, Mirko; Vollmer, Waldemar; Di Ventura, Barbara; Levin, Petra Anne
Pubblicato in: Molecular Microbiology, 2021, ISSN 0950-382X
Editore: Blackwell Publishing Inc.
DOI: 10.1111/mmi.14711

Carbohydrate-based adjuvants

Autori: Pilar Garcia-Vello, Immacolata Speciale, Fabrizio Chiodo, Antonio Molinaro, Cristina De Castro
Pubblicato in: Drug Discovery Today: Technologies, Numero 35-36, 2020, Pagina/e 57-68, ISSN 1740-6749
Editore: Elsevier BV
DOI: 10.1016/j.ddtec.2020.09.005

A biaryl-linked tripeptide from Planomonospora reveals a widespread class of minimal RiPP gene clusters

Autori: Mitja M. Zdouc, Mohammad M. Alanjary, Guadalupe S. Zarazúa, Sonia I. Maffioli, Max Crüsemann, Marnix H. Medema, Stefano Donadio, Margherita Sosio
Pubblicato in: Cell Chemical Biology, 2020, ISSN 2451-9456
Editore: Cell Chemical Biology
DOI: 10.1016/j.chembiol.2020.11.009

"Detection of in vivo Protein Interactions in All Bacterial Components by Förster Resonance Energy Transfer with the Superfolder mTurquoise2 ox-mNeongreen FRET Pair"

Autori: Nils Meiresonne, Elisa Consoli, Laureen Mertens, Tanneke den Blaauwen
Pubblicato in: BIO-PROTOCOL, Numero 9/23, 2019, ISSN 2331-8325
Editore: Bio-protocol
DOI: 10.21769/bioprotoc.3448

Lipoproteins in Gram-negative bacteria: new insights into their biogenesis, subcellular targeting and functional roles

Autori: Jessica El Rayes, Raquel Rodríguez-Alonso, Jean-François Collet
Pubblicato in: Current Opinion in Microbiology, Numero 61, 2021, Pagina/e 25-34, ISSN 1369-5274
Editore: Elsevier BV
DOI: 10.1016/j.mib.2021.02.003

Structural features and immunological perception of the cell surface glycans of Lactobacillus plantarum: a novel rhamnose-rich polysaccharide and teichoic acids

Autori: Pilar Garcia-Vello, Garima Sharma, Immacolata Speciale, Antonio Molinaro, Sin-Hyeog Im, Cristina De Castro
Pubblicato in: Carbohydrate Polymers, Numero 233, 2020, Pagina/e 115857, ISSN 0144-8617
Editore: Pergamon Press Ltd.
DOI: 10.1016/j.carbpol.2020.115857

The Lpt ABC transporter for lipopolysaccharide export to the cell surface

Autori: Paola Sperandeo, Alessandra M. Martorana, Alessandra Polissi
Pubblicato in: Research in Microbiology, Numero 170/8, 2019, Pagina/e 366-373, ISSN 0923-2508
Editore: Elsevier BV
DOI: 10.1016/j.resmic.2019.07.005

Planomonospora : A Metabolomics Perspective on an Underexplored Actinobacteria Genus

Autori: Mitja M. Zdouc, Marianna Iorio, Sonia I. Maffioli, Max Crüsemann, Stefano Donadio, Margherita Sosio
Pubblicato in: Journal of Natural Products, Numero 84/2, 2021, Pagina/e 204-219, ISSN 0163-3864
Editore: American Chemical Society
DOI: 10.1021/acs.jnatprod.0c00807

In vitro activity of rifabutin against 293 contemporary carbapenem-resistant Acinetobacter baumannii clinical isolates and characterization of rifabutin mode of action and resistance mechanisms

Autori: Vincent Trebosc, Birgit Schellhorn, Julian Schill, Valentina Lucchini, Jacqueline Bühler, Marilyne Bourotte, Jonathan J Butcher, Marc Gitzinger, Sergio Lociuro, Christian Kemmer, Glenn E Dale
Pubblicato in: Journal of Antimicrobial Chemotherapy, Numero 75/12, 2020, Pagina/e 3552-3562, ISSN 0305-7453
Editore: Oxford University Press
DOI: 10.1093/jac/dkaa370

The cell cycle regulator GpsB functions as cytosolic adaptor for multiple cell wall enzymes

Autori: Robert M. Cleverley, Zoe J. Rutter, Jeanine Rismondo, Federico Corona, Ho-Ching Tiffany Tsui, Fuad A. Alatawi, Richard A. Daniel, Sven Halbedel, Orietta Massidda, Malcolm E. Winkler, Richard J. Lewis
Pubblicato in: Nature Communications, Numero 10/1, 2019, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-08056-2

Thanatin Impairs Lipopolysaccharide Transport Complex Assembly by Targeting LptC–LptA Interaction and Decreasing LptA Stability

Autori: Elisabete C. C. M. Moura, Tiago Baeta, Alessandra Romanelli, Cedric Laguri, Alessandra M. Martorana, Emanuela Erba, Jean-Pierre Simorre, Paola Sperandeo, Alessandra Polissi
Pubblicato in: Frontiers in Microbiology, Numero 11, 2020, ISSN 1664-302X
Editore: Frontiers Media
DOI: 10.3389/fmicb.2020.00909

ActS activates peptidoglycan amidases during outer membrane stress in Escherichia coli

Autori: Carlos K. Gurnani Serrano, Matthias Winkle, Alessandra M. Martorana, Jacob Biboy, Niccolo Morè, Patrick Moynihan, Manuel Banzhaf, Waldemar Vollmer, Alessandra Polissi
Pubblicato in: Molecular Microbiology, 2021, ISSN 0950-382X
Editore: Blackwell Publishing Inc.
DOI: 10.1111/mmi.14712

The lipopolysaccharide transport (Lpt) machinery: A nonconventional transporter for lipopolysaccharide assembly at the outer membrane of Gram-negative bacteria

Autori: Paola Sperandeo, Alessandra M. Martorana, Alessandra Polissi
Pubblicato in: Journal of Biological Chemistry, Numero 292/44, 2017, Pagina/e 17981-17990, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.R117.802512

Copper inhibits peptidoglycan LD-transpeptidases suppressing β-lactam resistance due to bypass of penicillin-binding proteins

Autori: Katharina Peters, Manuel Pazos, Zainab Edoo, Jean-Emmanuel Hugonnet, Alessandra M. Martorana, Alessandra Polissi, Michael S. VanNieuwenhze, Michel Arthur, Waldemar Vollmer
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 115/42, 2018, Pagina/e 10786-10791, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.1809285115

Interaction of lipopolysaccharides at intermolecular sites of the periplasmic Lpt transport assembly

Autori: Cedric Laguri, Paola Sperandeo, Kevin Pounot, Isabel Ayala, Alba Silipo, Catherine M. Bougault, Antonio Molinaro, Alessandra Polissi, Jean-Pierre Simorre
Pubblicato in: Scientific Reports, Numero 7/1, 2017, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-017-10136-0

Superfolder mTurquoise2 ox optimized for the bacterial periplasm allows high efficiency in vivo FRET of cell division antibiotic targets

Autori: Nils Y. Meiresonne, Elisa Consoli, Laureen M. Y. Mertens, Anna Chertkova, Joachim Goedhart, Tanneke den Blaauwen
Pubblicato in: Molecular Microbiology, 2019, ISSN 0950-382X
Editore: Blackwell Publishing Inc.
DOI: 10.1111/mmi.14206

Solid State NMR Studies of Intact Lipopolysaccharide Endotoxin

Autori: Cedric Laguri, Alba Silipo, Alessandra M. Martorana, Paul Schanda, Roberta Marchetti, Alessandra Polissi, Antonio Molinaro, Jean-Pierre Simorre
Pubblicato in: ACS Chemical Biology, Numero 13/8, 2018, Pagina/e 2106-2113, ISSN 1554-8929
Editore: American Chemical Society
DOI: 10.1021/acschembio.8b00271

Peptidoglycan Remodeling Enables Escherichia coli To Survive Severe Outer Membrane Assembly Defect

Autori: Niccolò Morè, Alessandra M. Martorana, Jacob Biboy, Christian Otten, Matthias Winkle, Carlos K. Gurnani Serrano, Alejandro Montón Silva, Lisa Atkinson, Hamish Yau, Eefjan Breukink, Tanneke den Blaauwen, Waldemar Vollmer, Alessandra Polissi
Pubblicato in: mBio, Numero 10/1, 2019, Pagina/e e02729-18, ISSN 2150-7511
Editore: American Society for Microbiology
DOI: 10.1128/mbio.02729-18

Structural insight into the formation of lipoprotein-β-barrel complexes

Autori: Raquel Rodríguez-Alonso, Juliette Létoquart, Van Son Nguyen, Gwennaelle Louis, Antonio N. Calabrese, Bogdan I. Iorga, Sheena E. Radford, Seung-Hyun Cho, Han Remaut, Jean-François Collet
Pubblicato in: Nature Chemical Biology, Numero 16/9, 2020, Pagina/e 1019-1025, ISSN 1552-4450
Editore: Nature Publishing Group
DOI: 10.1038/s41589-020-0575-0

Structure of the O‐Antigen and the Lipid A from the Lipopolysaccharide of Fusobacterium nucleatum ATCC 51191

Autori: Pilar Garcia‐Vello, Flaviana Di Lorenzo, Dimitra Lamprinaki, Anna Notaro, Immacolata Speciale, Antonio Molinaro, Nathalie Juge, Cristina De Castro
Pubblicato in: ChemBioChem, Numero 22/7, 2021, Pagina/e 1252-1260, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000751

The Escherichia coli Outer Membrane β-Barrel Assembly Machinery (BAM) Anchors the Peptidoglycan Layer by Spanning It with All Subunits

Autori: Elisa Consoli, Jean-François Collet, Tanneke den Blaauwen
Pubblicato in: International Journal of Molecular Sciences, Numero 22/4, 2021, Pagina/e 1853, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22041853

Blocks in the pseudouridimycin pathway unlock hidden metabolites in the Streptomyces producer strain

Autori: Marianna Iorio, Sahar Davatgarbenam, Stefania Serina, Paolo Criscenzo, Mitja M. Zdouc, Matteo Simone, Sonia I. Maffioli, Richard H. Ebright, Stefano Donadio, Margherita Sosio
Pubblicato in: Scientific Reports, Numero 11/1, 2021, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-021-84833-2

Dissecting Colistin Resistance Mechanisms in Extensively Drug-Resistant Acinetobacter baumannii Clinical Isolates

Autori: Vincent Trebosc, Sarah Gartenmann, Marcus Tötzl, Valentina Lucchini, Birgit Schellhorn, Michel Pieren, Sergio Lociuro, Marc Gitzinger, Marcel Tigges, Dirk Bumann, Christian Kemmer
Pubblicato in: mBio, Numero 10/4, 2019, ISSN 2150-7511
Editore: American Society for Microbiology
DOI: 10.1128/mbio.01083-19

MreC and MreD balance the interaction between the elongasome proteins PBP2 and RodA

Autori: Xiaolong Liu, Jacob Biboy, Elisa Consoli, Waldemar Vollmer, Tanneke den Blaauwen
Pubblicato in: Plos Genetics, Numero 16(12), 2020, Pagina/e e1009276, ISSN 1553-7390
Editore: Public Library of Science
DOI: 10.1371/journal.pgen.1009276

Fat Matters for Bugs: How Lipids and Lipid Modifications Make the Difference in Bacterial Life

Autori: Paola Sperandeo, Alessandra Polissi, Emma De Fabiani
Pubblicato in: European Journal of Lipid Science and Technology, Numero 121/10, 2019, Pagina/e 1900204, ISSN 1438-7697
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/ejlt.201900204

Mutation and Suppressor Analysis of the Essential Lipopolysaccharide Transport Protein LptA Reveals Strategies To Overcome Severe Outer Membrane Permeability Defects in Escherichia coli

Autori: Federica A. Falchi, Elisa A. Maccagni, Simone Puccio, Clelia Peano, Cristina De Castro, Angelo Palmigiano, Domenico Garozzo, Alessandra M. Martorana, Alessandra Polissi, Gianni Dehò, Paola Sperandeo
Pubblicato in: Journal of Bacteriology, Numero 200/2, 2018, ISSN 0021-9193
Editore: American Society for Microbiology
DOI: 10.1128/jb.00487-17

Other (3)

Structure-function analyses of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation

Autori: J. A. Bryant, F. C. Morris, T. J. Knowles, R. Maderbocus, E. Heinz, G. Boelter, D. Alodaini, A. Colyer, P. J. Wotherspoon, K. A. Staunton, M. Jeeves, D. F. Browning, Y. R. Sevastsyanovich, T. J. Wells, A. E. Rossiter, V. N. Bavro, P. Sridhar, D. G. Ward, Z-S. Chong, C. Icke, A. Teo, S-S. Chng, View ORCID ProfileD. I. Roper, T. Lithgow, A. F. Cunningham, M. Banzhaf, M. Overduin, I. R. Henderson
Pubblicato in: BioRxiv, 2020
Editore: BioRxiv
DOI: 10.1101/2020.08.10.244616

Disorder is a critical component of lipoprotein sorting in Gram-negative bacteria

Autori: Jessica El Rayes, Joanna Szewczyk, Michael Deghelt, André Matagne, Bogdan I. Iorga, Seung-Hyun Cho, Jean-François Collet
Pubblicato in: BioRxiv, 2021
Editore: BioRxiv
DOI: 10.1101/2021.01.05.425367

Metabolomic investigation of the pseudouridimycin producer, a prolific streptomycete

Autori: Iorio, M., Davatgarbenam, S., Serina, S., Criscenzo, P., Zdouc, M.M., Simone, M., Maffioli, S.I., Ebright, R.H., Donadio, S. and Sosio, M.
Pubblicato in: BioRxiv, Numero 5. November 2020, 2020
Editore: BioRxiv
DOI: 10.1101/2020.11.05.369249

Thesis and dissertations (1)

Antibiotic drug development

Autori: Matias, C. S. S., Sommer, M. O. A., Ingmer, H., Weber, T., Polissi, A., & Olsen, J. E.
Pubblicato in: 2020
Editore: Technical University of Denmark