Designer recombinases for efficient and safe genome surgery

Project Information

GENSURGE

Grant agreement ID: 742133

DOI 10.3030/742133

Project closed

EC signature date 27 June 2017

Start date 1 January 2018

End date 30 June 2024

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 380 425,00

EU contribution € 2 380 425,00

Coordinated by

TECHNISCHE UNIVERSITAET DRESDEN
Germany

Publications

Peer reviewed articles (7)

Conformational dynamics promotes disordered regions from function-dispensable to essential in evolved site-specific DNA recombinases

Author(s): Carla Guillén-Pingarrón, Pedro M Guillem-Gloria, Anjali Soni, Gloria Ruiz-Gómez, Martina Augsburg, Frank Buchholz, Massimiliano Anselmi, M Teresa Pisabarro
Published in: Computational and Structural Biotechnology Journal, Issue Volume 20, 2022, Page(s) 989-1001, ISSN 2001-0370
Publisher: Research Network of Computational and Structural
DOI: 10.1016/j.csbj.2022.01.010

Pairing of single mutations yields obligate Cre-type site-specific recombinases

Author(s): Jenna Hoersten, Gloria Ruiz-Gómez, Felix Lansing, Teresa Rojo-Romanos, Lukas Theo Schmitt, Jan Sonntag, M Teresa Pisabarro, Frank Buchholz
Published in: Nucleic Acids Research, Issue Volume 50 Issue 2, 2022, Page(s) 1174–1186, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkab1240

A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination

Author(s): Madina Karimova, Oliver Baker, Aylin Camgoz, Ronald Naumann, Frank Buchholz, Konstantinos Anastassiadis
Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-32802-7

Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors

Author(s): Duran Sürün, Aksana Schneider, Jovan Mircetic, Katrin Neumann, Felix Lansing, Maciej Paszkowski-Rogacz, Vanessa Hänchen, Min Ae Lee-Kirsch, Frank Buchholz
Published in: Genes, Issue 11/5, 2020, Page(s) 511, ISSN 2073-4425
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes11050511

A heterodimer of evolved designer-recombinases precisely excises a human genomic DNA locus

Author(s): Felix Lansing, Maciej Paszkowski-Rogacz, Lukas Theo Schmitt, Paul Martin Schneider, Teresa Rojo Romanos, Jan Sonntag, Frank Buchholz
Published in: Nucleic Acids Research, Issue 48/1, 2019, Page(s) 472-485, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkz1078

Nearest-neighbor amino acids of specificity-determining residues influence the activity of engineered Cre-type recombinases

Author(s): Anjali Soni, Martina Augsburg, Frank Buchholz, M. Teresa Pisabarro
Published in: Scientific Reports, Issue 10/1, 2020, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-020-70867-5

Correction of a Factor VIII genomic inversion with designer-recombinases

Author(s): Felix Lansing, Liliya Mukhametzyanova, Teresa Rojo-Romanos, Kentaro Iwasawa, Masaki Kimura, Maciej Paszkowski-Rogacz, Janet Karpinski, Tobias Grass, Jan Sonntag, Paul Martin Schneider, Ceren Günes, Jenna Hoersten, Lukas Theo Schmitt, Natalia Rodriguez-Muela, Ralf Knöfler, Takanori Takebe & Frank Buchholz
Published in: Nature Communications, Issue 422 (2022), 2022, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-28080-7